Enhanced methane production of Chlorella vulgaris and Chlamydomonas reinhardtii by hydrolytic enzymes addition

Mahdy, Ahmed; Méndez, Lara; Ballesteros, Mercedes; González‐Fernández, Cristina

doi:10.1016/j.enconman.2014.04.097

Cited by 108 publications

(64 citation statements)

References 28 publications

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“…In this sense, the protein fraction was approximately double than the carbohydrates fraction. The macromolecular distribution attained for microalgae biomass and activated sludge is in good agreement with literature (Mottet et al, 2010;Mahdy et al, 2014a). Opposite, the carbohydrates fraction prevailed over the proteins measured in the primary sludge.…”

Section: Substrates Chemical Characterization Subjected To Anaerobic supporting

confidence: 88%

“…The methane production reported for the microalgae digestion as a sole substrate in that study was surprisingly low 54 mL g VS in À1 after 30 days. Indeed, the biogas methane content was as well surprisingly low (35-47%) compared with other biomethane production assays of C. vulgaris reported in literature (Mendez et al, 2014a,b;Mahdy et al, 2014a). Wang et al (2013) observed the lowest biogas production using microalgae as a sole substrate while the volatile solids reduction of this sample was higher than the one observed for activated sludge.…”

Section: Biomethane Potential Assays Of Codigested Substratescontrasting

confidence: 49%

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Algaculture integration in conventional wastewater treatment plants: Anaerobic digestion comparison of primary and secondary sludge with microalgae biomass

Mahdy

Méndez

Ballesteros

et al. 2015

Bioresource Technology

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Section: Substrates Chemical Characterization Subjected To Anaerobic supporting

confidence: 88%

Section: Biomethane Potential Assays Of Codigested Substratescontrasting

confidence: 49%

Algaculture integration in conventional wastewater treatment plants: Anaerobic digestion comparison of primary and secondary sludge with microalgae biomass

Mahdy

Méndez

Ballesteros

et al. 2015

Bioresource Technology

Self Cite

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“…To date, results showed how the methane yield of Chlorella vulgaris was increased by 70% with cellulase (Onozuka) and a hemicellulose mix (Macerozyme) (Wieczorek et al, 2014). Similarly, the methane yield of the same microalgae species was increased by 86% with carbohydrolase and protease (Mahdy et al, 2014). For the filamentous microalgae Rhizoclonium sp., an enzyme mix composed by amylase, protease, lipase, xylanase and cellulase enhanced the methane yield by 30% (Ehimen et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Improving biogas production from microalgae by enzymatic pretreatment

Passos

Hom-Díaz

Blánquez

et al. 2016

Bioresource Technology

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Please cite this article as: Passos, F., Hom-Diaz, A., Blanquez, P., Vicent, T., Ferrer, I., Improving biogas production from microalgae by enzymatic pretreatment, Bioresource Technology (2015), doi: http://dx.doi.org/10. 1016/ j.biortech.2015.08.084 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Improving biogas production from microalgae by enzymatic pretreatment AbstractIn this study, enzymatic pretreatment of microalgal biomass was investigated under different conditions and evaluated using biochemical methane potential (BMP) tests. Cellulase, glucohydrolase and an enzyme mix composed of cellulase, glucohydrolase and xylanase were selected based on the microalgae cell wall composition (cellulose, hemicellulose, pectin and glycoprotein). All of them increased organic matter solubilisation, obtaining high values already after 6 hours of pretreatment with an enzyme dose of 1% for cellulase and the enzyme mix. BMP tests with pretreated microalgae showed a methane yield increase of 8 and 15% for cellulase and the enzyme mix, respectively. Prospective research should evaluate enzymatic pretreatments in continuous anaerobic reactors so as to estimate the energy balance and economic cost of the process.

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“…Protease activity is largely dependent on pH and temperature and varies widely from one enzyme to another. Common proteases with potential for microalgae cell wall/membrane disruption include trypsin, lysozyme, collagenases, papain, and autolysins (Gerken et al 2013;Mahdy et al 2014a;Horst et al 2012). Preliminary screening of enzymes indicated that proteases may catalyze cell wall disruption for C. vulgaris cells (Mahdy et al 2014b).…”

Section: Proteasesmentioning

confidence: 99%

Green microalgae biomolecule separations and recovery

Dixon

Wilken

2018

Bioresour. Bioprocess.

102

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Microalgae biomass has garnered significant attention as a renewable energy feedstock and alternative to petroleumbased fuels. The diverse metabolism of green microalgae species additionally provides opportunities for recovery of products for feed, food, nutraceutical, cosmetic, and biopharmaceutical industries. Recently, the concept of using microalgae as part of a biorefinery model has been adopted in place of refinery methods focused on recovering one target product. This has led to producers exploring co-production of high value and high volume products in an effort to improve process economics. With numerous potential products and applications, the biomass source or specific strain must be carefully selected to accumulate extractable levels of the target molecule(s). It is additionally imperative to understand the morphology and metabolism of the selected strain to cost-effectively manage all stages of commercial production. This review will focus specifically on microalgae in the division of Chlorophyta, or green algae and their extracellular matrices (ECM), potential for commercial products, and finally describe a holistic approach for biomolecule extraction and recovery. Additionally, cell disruption and fractionation methods for recovery of biomolecules for commercial products are highlighted along with an alternative method, aqueous enzymatic processing for multiple biomolecule extraction and recovery from green microalgae. An emphasis is placed on connecting the morphological characteristics of microalgae ECM or organelle membranes to implications on separation and purification technologies.

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Enhanced methane production of Chlorella vulgaris and Chlamydomonas reinhardtii by hydrolytic enzymes addition

Cited by 108 publications

References 28 publications

Algaculture integration in conventional wastewater treatment plants: Anaerobic digestion comparison of primary and secondary sludge with microalgae biomass

Algaculture integration in conventional wastewater treatment plants: Anaerobic digestion comparison of primary and secondary sludge with microalgae biomass

Improving biogas production from microalgae by enzymatic pretreatment

Green microalgae biomolecule separations and recovery

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