Enhanced methane production and hydrocarbon removal from petroleum refinery sludge after Pseudomonas putida pretreatment and process scale-up

Choudhury, Shinjini Paul; Panda, Sugato; Haq, Izharul; Kalamdhad, Ajay S.

doi:10.1016/j.biortech.2021.126127

Cited by 19 publications

(4 citation statements)

References 40 publications

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“…The pretreated SB with KKU-MC1 has a high sCOD, indicating that it is highly biodegradable, enhancing the sCOD up to 42.1% compared with the control at 5 days. The increased sCOD found in this study was comparable to that of Choudhury et al 33 . (7,680 mg/L), who biologically pretreated petroleum refinery sludge with Pseudomonas putida .…”

Section: Resultssupporting

confidence: 92%

Taxonomic and enzymatic basis of the cellulolytic microbial consortium KKU-MC1 and its application in enhancing biomethane production

Wongfaed

O‐Thong

Sittijunda

et al. 2023

Sci Rep

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Lignocellulosic biomass is a promising substrate for biogas production. However, its recalcitrant structure limits conversion efficiency. This study aims to design a microbial consortium (MC) capable of producing the cellulolytic enzyme and exploring the taxonomic and genetic aspects of lignocellulose degradation. A diverse range of lignocellulolytic bacteria and degrading enzymes from various habitats were enriched for a known KKU-MC1. The KKU-MC1 was found to be abundant in Bacteroidetes (51%), Proteobacteria (29%), Firmicutes (10%), and other phyla (8% unknown, 0.4% unclassified, 0.6% archaea, and the remaining 1% other bacteria with low predominance). Carbohydrate-active enzyme (CAZyme) annotation revealed that the genera Bacteroides,Ruminiclostridium,Enterococcus, and Parabacteroides encoded a diverse set of cellulose and hemicellulose degradation enzymes. Furthermore, the gene families associated with lignin deconstruction were more abundant in the Pseudomonas genera. Subsequently, the effects of MC on methane production from various biomasses were studied in two ways: bioaugmentation and pre-hydrolysis. Methane yield (MY) of pre-hydrolysis cassava bagasse (CB), Napier grass (NG), and sugarcane bagasse (SB) with KKU-MC1 for 5 days improved by 38–56% compared to non-prehydrolysis substrates, while MY of prehydrolysed filter cake (FC) for 15 days improved by 56% compared to raw FC. The MY of CB, NG, and SB (at 4% initial volatile solid concentration (IVC)) with KKU-MC1 augmentation improved by 29–42% compared to the non-augmentation treatment. FC (1% IVC) had 17% higher MY than the non-augmentation treatment. These findings demonstrated that KKU-MC1 released the cellulolytic enzyme capable of decomposing various lignocellulosic biomasses, resulting in increased biogas production.

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Section: Resultssupporting

confidence: 92%

Taxonomic and enzymatic basis of the cellulolytic microbial consortium KKU-MC1 and its application in enhancing biomethane production

Wongfaed

O‐Thong

Sittijunda

et al. 2023

Sci Rep

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“…The type II, OTU_22 and OTU_30 of the genus Bacillus , can produce surfactants to promote the formation of methane hydrate and enhance methane migration 94 , with thermostability and salt-tolerance 95 . Surfactants derived from OTU_31 and OTU_32 of the genus Pseudomonas can emulsify aliphatic compounds and aromatic hydrocarbons and cooperate with primary producers to promote methanogenesis 96 . These bacteria abundance are high in samples Y-3, S-1, and S-2, indicating that the methane migration associated with microbial community also enhanced methane enrichment in Yuejin and Shizigou profiles.…”

Section: Resultsmentioning

confidence: 99%

Microbial communities and their roles in the Cenozoic sulfurous oil reservoirs in the Southwestern Qaidam Basin, Western China

Jiao

Wang³

et al. 2023

Sci Rep

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The latest discovery of sulfurous natural gas marked a breakthrough in the Cenozoic natural gas exploration in the southwestern margin of Qaidam Basin. The 16S rRNA analyses were performed on the crude oil samples from H2S-rich reservoirs in the Yuejin, Shizigou and Huatugou profiles, to understand the sulfurous gas origin, which was also integrated with carbon and hydrogen isotopes of alkane and sulfur isotopes of H2S collected from the Yingxiongling Area. Results show that the microorganisms in samples can survive in the hypersaline reservoirs, and can be classified into multiple phyla, including Proteobacteria, Planctomycetes, Firmicutes, Bacteroidetes, and Haloanaerobiaeota. Methanogens are abundant in all of the three profiles, while sulfate-reducing bacteria are abundant in Yuejin and Huatugou profiles, contributing to the methane and H2S components in the natural gas. The carbon, hydrogen and sulfur isotopes of sulfurous natural gas in the Yingxiongling Area show that the natural gas is a mixture of coal-type gas and oil-type gas, which was primarily derived from thermal degradation, and natural gas from the Yuejin and Huatugou profiles also originated from biodegradation. The isotopic analysis agrees well with the 16S rRNA results, i.e., H2S-rich natural gas from the Cenozoic reservoirs in the southwest margin of the Qaidam Basin was primarily of thermal genesis, with microbial genesis of secondary importance.

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“…GC-MS and AK6 community structure analysis confirmed biodegradation of oily sludge hydrocarbons, which is in line with the fact that the AK6 consortium was originally obtained from hydrocarbon-polluted soil ( Ismail et al, 2016 ). Different species of the dominant genera ( Klebsiella , Pseudomonas and Stenotrophomonas ) in the oily sludge cultures of AK6 were frequently reported for their hydrocarbons, crude oil, and oily sludge biodegradation capabilities, and many of them were isolated from hydrocarbon-polluted environments ( Chebbi et al, 2017 ; Behera et al, 2020 ; Bilen Ozyurek and Seyis Bilkay, 2020 ; Koolivand et al, 2020 ; Medić et al, 2020 ; Hentati et al, 2021a ; Muneeswari et al, 2021 ; Choudhury et al, 2022 ). Furthermore, since no exogenous sulfur source was added to the batch cultures and chemical analysis revealed the presence of sulfur in the oily sludge, it can be hypothesized that AK6 relied on the oily sludge as the sulfur source, which is corroborated by the reported ability of AK6 to utilize different thiophenic organosulfur compounds commonly found in crude oil, as the sole sulfur source ( Ismail et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Pseudomonas spp. have been widely described as efficient hydrocarbon degraders ( Medić et al, 2020 ; Choudhury et al, 2022 ). Studies on numerous Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Biotreatment of oily sludge by a bacterial consortium: Effect of bioprocess conditions on biodegradation efficiency and bacterial community structure

et al. 2022

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We studied the biodegradation of oily sludge generated by a petroleum plant in Bahrain by a bacterial consortium (termed as AK6) under different bioprocess conditions. Biodegradation of petroleum hydrocarbons in oily sludge (C11-C29) increased from 24% after two days to 99% after 9 days of incubation in cultures containing 5% (w/v) of oily sludge at 40°C. When the nitrogen source was excluded from the batch cultures, hydrocarbon biodegradation dropped to 45% within 7 days. The hydrocarbon biodegradation decreased also by increasing the salinity to 3% and the temperature above 40°C. AK6 tolerated up to 50% (w/v) oily sludge and degraded 60% of the dichloromethane-extractable oil fraction. Illumina-MiSeq analyses revealed that the AK6 consortium was mainly composed of Gammaproteobacteria (ca. 98% of total sequences), with most sequences belonging to Klebsiella (77.6% of total sequences), Enterobacter (16.7%) and Salmonella (5%). Prominent shifts in the bacterial composition of the consortium were observed when the temperature and initial sludge concentration increased, and the nitrogen source was excluded, favoring sequences belonging to Pseudomonas and Stenotrophomonas. The AK6 consortium is endowed with a strong oily sludge tolerance and biodegradation capability under different bioprocess conditions, where Pseudomonas spp. appear to be crucial for hydrocarbon biodegradation.

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Enhanced methane production and hydrocarbon removal from petroleum refinery sludge after Pseudomonas putida pretreatment and process scale-up

Cited by 19 publications

References 40 publications

Taxonomic and enzymatic basis of the cellulolytic microbial consortium KKU-MC1 and its application in enhancing biomethane production

Taxonomic and enzymatic basis of the cellulolytic microbial consortium KKU-MC1 and its application in enhancing biomethane production

Microbial communities and their roles in the Cenozoic sulfurous oil reservoirs in the Southwestern Qaidam Basin, Western China

Biotreatment of oily sludge by a bacterial consortium: Effect of bioprocess conditions on biodegradation efficiency and bacterial community structure

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