Enhanced loading of glucose oxidase on polyaniline films based on anion exchange

Abstract

ABSTRACT:The temporal aspects of anion self-exchange in electrochemically prepared polyaniline films have been experimentally investigated. The exchange of bulkier tosylate-ferricyanide ion with Cl 0 ion has been monitored by photometry and electrochemical techniques. The relative changes in porosity brought about by self-exchange have been experimentally determined to be 323 and 2125/k in tosylate-exchanged and ferricyanide-exchanged polyaniline films, respectively. Scanning electron microscopy has been used to delineate the surface morphology of polyaniline films. It is seen that the polyaniline films exhibit enhanced loading of glucose oxidase after a self-ion exchange, and, hence, they can be used for the fabrication of a third-generation glucose biosensor.

Recent advances in polyaniline based biosensors

“…PANI-tosylate and PANI ferricynide films, prepared by self-ionexchange in HCl, has been investigated by Verghese et al (1998) for high GOx loading due to its significant change in the film porosity. The importance of this ion self-exchange technique has been used to develop reagentless PANI-based biosensor.…”

Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films

“…: +91 11 25734273; fax: +91 11 25726938. into an electrical signal resulting into signal amplification and elimination of electrode fouling. It has been reported by many researchers that the polyaniline can be used as a sensing element for estimation of various analytes [8,9].…”

Immobilization of tyrosinase on poly(indole-5-carboxylic acid) evidenced by electrochemical and spectroscopic methods

“…The simplest way of enzyme immobilization on the CP surface is the physical adsorption, where the binding forces involved hydrogen bonds, coulombic interaction, etc. [2,7,9,10].…”