Enhanced lipoxygenase activity is involved in barley root tip swelling induced by cadmium, auxin or hydrogen peroxide

Alemayehu, Aster; Bočová, Beáta; Zelinová, Veronika; Mistrı́k, Igor; Tamás, Ladislav

doi:10.1016/j.envexpbot.2013.06.004

Cited by 13 publications

(6 citation statements)

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“…In line with our results regarding the activation of 13-LOX, it was reported that the enhancement of lipoxygenase activity showed to be involved in barley root tip swelling induced by cadmium, auxin or hydrogen peroxide (Alemayehu et al, 2013). Very recently, it was shown that 13-LOX has a key role in chloroplast breakdown as part of programmed cell death in senescing plants (Springera et al, 2016).…”

Section: Discussionsupporting

confidence: 92%

Specific Caleosin/Peroxygenase and Lipoxygenase Activities Are Tissue-Differentially Expressed in Date Palm (Phoenix dactylifera L.) Seedlings and Are Further Induced Following Exposure to the Toxin 2,3,7,8-tetrachlorodibenzo-p-dioxin

et al. 2017

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Two caleosin/peroxygenase isoforms from date palm, Phoenix dactylifera L., PdCLO2 and PdCLO4, were characterized with respect to their tissue expression, subcellular localization, and oxylipin pathway substrate specificities in developing seedlings. Both PdCLO2 and PdCLO4 had peroxygenase activities that peaked at the mid-stage (radicle length of 2.5 cm) of seedling growth and were associated with the lipid droplet (LD) and microsomal fractions. Recombinant PdCLO2 and PdCLO4 proteins heterologously expressed in yeast cells were localized in both LD and microsomal fractions. Each of the purified recombinant proteins exhibited peroxygenase activity but they were catalytically distinct with respect to their specificity and product formation from fatty acid epoxide and hydroxide substrates. We recently showed that date palm CLO genes were upregulated following exposure to the potent toxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (Hanano et al., 2016), and we show here that transcripts of 9- and 13-lipoxygenase (LOX) genes were also induced by TCDD exposure. At the enzyme level, 9-LOX and 13-LOX activities were present in a range of seedling tissues and responded differently to TCDD exposure, as did the 9- and 13-fatty acid hydroperoxide reductase activities. This demonstrates that at least two branches of the oxylipin pathway are involved in responses to the environmental organic toxin, TCDD in date palm.

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Section: Discussionsupporting

confidence: 92%

Specific Caleosin/Peroxygenase and Lipoxygenase Activities Are Tissue-Differentially Expressed in Date Palm (Phoenix dactylifera L.) Seedlings and Are Further Induced Following Exposure to the Toxin 2,3,7,8-tetrachlorodibenzo-p-dioxin

et al. 2017

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“…In Arabidopsis root apex, a marked IAA accumulation was observed afBrought to you by | New York University Bobst Library Technical Services Authenticated Download Date | 6/13/15 3:15 PM ter Cu exposure, which was probably responsible for the reorganization of root system architecture such as root hair and lateral root formation (Lequeux et al 2010). In addition to morphogenic changes, it has been previously reported that exposure of roots to either Cd or IAA evoked similar changes in enzyme activities such as inhibition of AAO or stimulation of LOX activity (Zelinová et al 2011;Alemayehu et al 2013). Taken together, the results of this study and previous observations strongly indicate that Cd-induced morphogenic responses and some changes in enzyme activities are induced by changes in IAA redistribution and accumulation.…”

Section: Discussionsupporting

confidence: 76%

“…Our previous studies have revealed that Cd-induced H 2 O 2 generation reaches its maximum 3 h, while changes in enzymes activity and root morphology fully developed only 6 h after the short-term treatment with Cd (Zelinová et al 2011;Tamás et al 2012;Alemayehu et al 2013;Zelinová et al 2013). Therefore, in this study we analysed the effect of TIBA, 1-NOA or PCIB on the Cdinduced alteration in IAA content and ROS production in barley root tip 3 h after the short-term treatments.…”

Section: Introductionmentioning

confidence: 93%

“…On the other hand, recent studies indicate that in many cases this stressinduced LOX activity is associated with the initiation of oxylipin pathways (Cuypers et al 2011). In barley, probably LOX-generated oxylipins are involved in the induction of root swelling after Cd, IAA or hydrogen peroxide treatment (Alemayehu et al 2013). The similarities between Cd-and IAA-induced morphogenic and antioxidant responses in roots indicate the signalling function of IAA in these processes.…”

Section: Introductionmentioning

confidence: 97%

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Cadmium-induced reactive oxygen species generation, changes in morphogenic responses and activity of some enzymes in barley root tip are regulated by auxin

et al. 2015

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Morphogenic responses, ROS generation and changes in enzymes activity induced by the short-term exposure of barley roots to Cd were very similar to those of triiodobenzoic acid (TIBA) treatment. Both Cd and TIBA markedly increased the amount of IAA in root apex. In addition, the treatment of roots with TIBA before Cd treatment had an additive effect on morphogenic responses, changes in enzymes activity and ROS generation. In turn, the application of auxin efflux/influx inhibitor 1-naphthoxyacetic acid (1-NOA) before Cd treatment significantly reduced the Cd-induced ROS generation, root growth inhibition and enzymes activity in spite of the elevated level of IAA in the root apex. The auxin signalling inhibitor p-chlorophenoxyisobutyric acid (PCIB) nearly fully eliminated some of these Cd-induced responses. These results indicated that the short-term Cd treatment-induced ROS generation, morphogenic responses and activity of some enzymes can be attributed to the disturbance of IAA homeostasis in root apex. While auxin efflux inhibitor TIBA evoked similar response to Cd, auxin efflux/influx inhibitor 1-NOA attenuated and auxin signalling inhibitor PCIB blocked these Cd-induced responses suggesting that apart from the elevated level of IAA in root apex its enter and accumulation inside of until closely undetermined cells or tissues is a prerequisite for the induction of characteristic root responses to short-term Cd exposure.

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“…In addition, using SNP as a NO donor revealed that NO is probably responsible for the stimulation of root growth under stress conditions without affecting the Cd-induced ROS production in the proximal part of elongation and in the differentiation root zones. It has been previously demonstrated that application of antioxidant had some alleviating effect on Cd-induced root growth inhibition but it was much less efficient in comparison to SNP (Alemayehu et al 2013). It is also supported by the observation that NO donor SNP did not affect the Cd-induced radial expansion of root apex, but caused its marked lengthening due to the NO-induced longitudinal cell elongation.…”

Section: Discussionsupporting

confidence: 66%

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

et al. 2015

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Background and Aims The objective of this study was to analyse the role of nitric oxide (NO) in relation to reactive oxygen species (ROS) production and root growth inhibition in the early response of barley root tip to cadmium. Methods We used cell-permeable NO-binding dye DAF-2DA for the NO localization and cellimpermeable dye DAR-4M for the quantification of NO in barley root apex. Results A considerable increase in NO production was detected in the transition zone of root tips already 30 min after the immersion of barley roots into 15 μM Cd. This Cd-induced NO generation further increased during the recovery period and reached its maximum level 2 h after the short-term treatment. At this stage an enhanced Cd-induced NO generation was observed in the meristematic, transition and in the beginning of elongation zone in comparison with control roots. Inhibitor of NO synthase activity markedly reduced root growth in control seedlings and had a synergistic growth inhibitory effect with Cd. On the contrary, this Cdinduced root growth inhibition was markedly alleviated by the NO donor. Exposure of roots to Cd markedly increased the production of ROS in the proximal elongation zone of root. While NO did not affect ROS generation, inhibition of its synthesis slightly decreased ROS generation in both control and Cd-treated roots. Conclusions Our results revealed that enhanced NO production is a very early response of barley root to Cd stress and it is involved in the regulation of root growth mitigating Cd-induced root growth inhibition.

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Enhanced lipoxygenase activity is involved in barley root tip swelling induced by cadmium, auxin or hydrogen peroxide

Cited by 13 publications

References 53 publications

Specific Caleosin/Peroxygenase and Lipoxygenase Activities Are Tissue-Differentially Expressed in Date Palm (Phoenix dactylifera L.) Seedlings and Are Further Induced Following Exposure to the Toxin 2,3,7,8-tetrachlorodibenzo-p-dioxin

Specific Caleosin/Peroxygenase and Lipoxygenase Activities Are Tissue-Differentially Expressed in Date Palm (Phoenix dactylifera L.) Seedlings and Are Further Induced Following Exposure to the Toxin 2,3,7,8-tetrachlorodibenzo-p-dioxin

Cadmium-induced reactive oxygen species generation, changes in morphogenic responses and activity of some enzymes in barley root tip are regulated by auxin

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

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