Enhanced lipid content in Chlorella sp. FC2 IITG via high energy irradiation mutagenesis

Muthuraj, Muthusivaramapandian; Selvaraj, Baskar; Palabhanvi, Basavaraj; Kumar, Vikram; Das, Debasish

doi:10.1007/s11814-018-0180-z

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…This particular species has an efficient to trap carbon from the atmosphere, and therefore mitigate CO2 gas emission from the industries. Further, Muthuraj et al [12] did show high lipid content in Chlorella p., FC2 IITG, and therefore, authors in this study demonstrated its potential for biodiesel production. Similarly, around 59 species of microalgae were isolated by Lahan et al [15,16] from different freshwater bodies in Dhemaji district.…”

Section: Microalgal Diversity Of Northeast Indiasupporting

confidence: 65%

An Emerging Aquatic Green Gold for Food and Medicine: A Review of Algae From North East India

Medhi

Kalita

2020

Int J Pharm Pharm Sci

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Given that proper nutrition is a growing concern for rising global populations, sustainable sources of nutritional value are in need. The food product or food derived from nutrients is called nutraceuticals which not only rarely supplement food but also make the treatment or prevention of a disorder and or disease. Algae are a diverse community of autotrophic organisms with the capable of fix atmospheric CO2, efficiently use light energy, ability to grow rapidly and compare to vascular plants, and algae produce more biomass per acre. More than two thousand years, algae are used for the treatment of different ailments and also used as a potential source of food. Due to the characteristics of rapid growth and capable of producing diverse nutritional compound, algae are largely used in dietary supplements and nutraceuticals field. Many kinds of algae have been reported several health benefits from improving the immune system to combat cancer and heart disease. With this background, this current review aims to evaluate the health-promoting effect of Chlorella, Haematococcus, Spirulina, Ankistrodesmus, Botryococcus and Scenedesmus in North East India.

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Section: Microalgal Diversity Of Northeast Indiasupporting

confidence: 65%

An Emerging Aquatic Green Gold for Food and Medicine: A Review of Algae From North East India

Medhi

Kalita

2020

Int J Pharm Pharm Sci

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“…Physical method includes the exposure of organism to radiation such as X-rays, gamma and ultraviolet (UV) rays (Zayadan et al, 2014 ) or atmospheric and room temperature plasma (ARTP). UV radiation at 250–290 nm can either induce deletion of A–T base pairs in the DNA or promote thymine dymers (Muthuraj et al, 2019 ). On the other hand, chemical method such as ethyl methane sulfonate (EMS) and nitrosomethyl guadinine (Bird & Neuffer, 1987 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of isolated UV-C-irradiated mutants of microalga Chlorella vulgaris for future biofuel application

Carino

Vital

2022

Environ Dev Sustain

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Microalgae-based biofuel is considered as one of the most promising sources of alternative energy because it is sustainable and does not pose threats to the environment and food security. However, attempts in improving microalgal strains to attain the ideal characteristics for biofuel application are yet to unravel. In this study, random UV-C mutagenesis was employed to generate starch-deficient mutants of indigenous Chlorella vulgaris to enhance its productivity. Out of 872 colonies, two isolated mutants (cvm5 and cvm6) were isolated and showed significant increase in cell concentrations by > 1.47-fold and > 1.04-fold, respectively. However, mutant cells exhibited smaller in size which might contributed to the significant decrease in their biomass. Moreover, gathered data revealed that the total lipid content of cvm5 was enhanced significantly (75%, > 1.3-fold increase). Additionally, triacylglycerol (TAG) content of the said mutant constitutes 48% of the dry cell weight (DCW) while cvm6 consist of 41% of the DCW. These promising and novel findings suggest that the two generated and isolated mutants are good candidates for future commercial biofuel production, especially in the Philippines. In addition, these findings may contribute on the prior knowledge of the usage of UV-C for microalgal strain development.

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“…This shows a drastic increase in the neutral lipid content with a concomitant reduction in the protein, carbohydrate, and chlorophyll levels within the biomass, thus explaining the rearrangement of macromolecular compositions to form TAG. Besides, activity screening of enzymes such as ADP-gluco-pyro-phosphorylase (AGPase), acetyl-CoA carboxylase (ACCase), glycerol-3-phosphate dehydrogenase, and diacyl-glycerol transferase showed varied activity under nutrient-limitation and replete conditions in FC2 (Muthuraj et al, 2019). For instance, the activity of AGPase was reduced with a concomitant increase in ACCase, suggesting the reduction of fluxes towards carbohydrate biosynthesis and associated redirection of carbon towards TAG biosynthesis under nitrogen limitation conditions (Muthuraj et al, 2019).…”

Section: Impaired Carbon Dioxide Fixationmentioning

confidence: 99%

“…Besides, activity screening of enzymes such as ADP-gluco-pyro-phosphorylase (AGPase), acetyl-CoA carboxylase (ACCase), glycerol-3-phosphate dehydrogenase, and diacyl-glycerol transferase showed varied activity under nutrient-limitation and replete conditions in FC2 (Muthuraj et al, 2019). For instance, the activity of AGPase was reduced with a concomitant increase in ACCase, suggesting the reduction of fluxes towards carbohydrate biosynthesis and associated redirection of carbon towards TAG biosynthesis under nitrogen limitation conditions (Muthuraj et al, 2019). Apart from their energy production role, these proteins, amino acids, and membrane lipids play an essential role as intermediates in replenishing the tricarboxylic acid (TCA) cycle for carbon recycling, and energy and reductant generation to meet the enhanced synthesis of fatty acids/TAG.…”

Section: Impaired Carbon Dioxide Fixationmentioning

confidence: 99%

Systematic metabolome profiling and multi-omics analysis of the nitrogen-limited non-model oleaginous algae for biorefining

et al. 2021

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Oleaginous microalga Chlorella is a promising microbial cell factory for producing lipids, transesterifiable to biodiesel, and phytochemical, high-value molecules (HVMs). To better understand the stress-induced oleaginous mechanism of Chlorella sp. FC2 IITG that closely matches with Chlorella sorokiniana based on 18s rRNA gene sequence, we performed 'Algomics' by systematically integrating metabolomics and proteomics data in response to time-resolved nitrogen-limitation: 40 h (mild), 88 h (moderate), and 120 h (severe). Ten HVMs belonging to four biological classes: triacylglycerol (TAG), polyunsaturated fatty acids (PUFA), phytosterols, and terpenoids were annotated using untargeted metabolomics and MS/MS fragmentation pattern match to the spectral library. In particular, the study evidenced 4× and 6× increased accumulation of two different PUFA: 9(S)HpOTrE and dihomo-gamma-linolenic acid, respectively, in nitrogen-limitation conditions. Co-extraction of TAG and PUFA could lower the biodiesel production cost for feasible commercialization. The investigation found a maximum accumulation of TAG 59:10 at 40 h, while that for TAG 54:4 was recorded at 88 h, which suggests different TAG species could be induced by regulating nitrogen-limitation severity. Elevated ꞵ-oxidation, glycolysis, and tricarboxylic acid (TCA) cycle identified in proteomics analysis could provide the substrates, phosphoenolpyruvate, pyruvate, and acetyl CoA, for different phytochemical accumulation in response to nitrogen limitation. The multiomics data unraveled a metabolic tug-of-war ongoing between biomass and storage lipid (TAG) accumulation during nitrogen-limitation, which involved multiple processes including hindered CO2 fixation, the supply of energy, reductants, and carbon reallocation from proteins and membrane lipids. These ﬁndings provide distinct oleaginous mechanisms in non-model microalgae, Chlorella sp. FC2 IITG, and engineerable targets for microalgal trait improvements.

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Enhanced lipid content in Chlorella sp. FC2 IITG via high energy irradiation mutagenesis

Cited by 13 publications

References 32 publications

An Emerging Aquatic Green Gold for Food and Medicine: A Review of Algae From North East India

An Emerging Aquatic Green Gold for Food and Medicine: A Review of Algae From North East India

Characterization of isolated UV-C-irradiated mutants of microalga Chlorella vulgaris for future biofuel application

Systematic metabolome profiling and multi-omics analysis of the nitrogen-limited non-model oleaginous algae for biorefining

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