Enhanced intimal thickening of expanded polytetrafluoroethylene grafts coated with fibrin or fibrin-releasing vascular endothelial growth factor in the pig carotid artery interposition model

Walpoth, Beat H.; Zammaretti, Prisca; Çıkırıkçıoğlu, Mustafa; Khabiri, Ebrahim; Djebaili, M. Karim; Pache, Jean Claude; Tille, Jean Christophe; Aggoun, Yacine; Morel, Denis R.; Kalangos, Afksendiyos; Hubbell, Jeffrey A.; Zisch, Andreas H.

doi:10.1016/j.jtcvs.2007.01.029

Cited by 60 publications

(35 citation statements)

References 28 publications

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“…Synthetic, non-degradable polymers can exhibit the opposite trend: strong mechanical properties, but poor biocompatibility. 33,49 We first showed that smooth muscle cell viability on the electrospun scaffolds investigated was not strongly dependent on scaffold processing (Fig. 3).…”

Section: Discussionmentioning

confidence: 94%

“…Unfortunately, in vivo small (<6 mm)-diameter synthetic grafts often exhibit compliance mismatch, rapid plaque formation, and occlusion of the lumen. 33,49 To provide a replacement vessel with biomimetic function and properties, tissue-engineering strategies have been investigated such as cell sheets or cells cultured on biopolymers, such as collagen, to form engineered vessels. 11,21,50 A key component of these strategies is promoting proper cell organization and orientation within the vessel, especially in the tunica media (middle layer) of the vessel.…”

Section: Introductionmentioning

confidence: 99%

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Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

et al. 2011

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For tissue-engineered vascular grafts to reach their full potential, three-dimensional (3D) cellular micro-integration will be necessary. In this study, we utilize femtosecond laser ablation to produce microchannels inside electrospun polycaprolactone (PCL) scaffolds. These microchannels potentially provide spatially controlled cell distributions approaching those observed in vivo. The ability of such laser-ablated microchannels to direct cell seeding was evaluated. The dimensions chosen were 100 μm wide, 100 μm deep and 10 mm long. Femtosecond laser ablation successfully produced these microchannels in the scaffolds without substantially altering the ~900 nm diameter fibers. Flow within these microchannels was studied by injecting fluorescent polystyrene bead solutions. Direct measurement of bead motion yielded an inlet velocity of 2.78 cm s(-1). This was used for modeling two-dimensional (2D) flow using computational fluid dynamics to estimate flow profiles within the microchannel. Successful demonstrations of bead flow were followed by seeding of 500,000 human coronary artery smooth muscle cells (HCASMCs) in proliferative medium at a rate of ~500 μL min(-1). Confocal microscopy and scanning electron microscopy confirmed that the HCASMCs were seeded down the full 10-mm length of the microchannel and stayed within its boundaries. Both nuclei and F-actin were observed within the seeded cells. The presence of F-actin filaments shows that the cells were adhered strongly to the scaffold and remained viable throughout the culture. The concept of "vascular wall engineering" producing intricate cell seeding through microchannels produced via femtosecond laser ablation was validated.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

et al. 2011

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“…Fibrin has been proposed as a coating: results have been either discouraging (Mooney DJ et al 1996;Kim BS et al 1998;Wake MC et al 1996) or encouraging as regards anti-adhesive action in platelets and perlecan (Mooney DJ et al 1996;Kim BS et al 1998;Wake MC et al 1996); one article reported an elastin-like recombining protein [84,85,88]. Single peptides like RGD, cyclic RGD (Mooney DJ et al 1996;Kim BS et al 1998;Wake MC et al 1996) have also been proposed, but only in vitro studies are available (Walpoth BH et al 2007;Lord MS et al 2009;Jordan SW et al 2007;Tang C et al 2009;Larsen CC et al 2007).…”

Section: Permanent Materialsmentioning

confidence: 99%

New Developments in Tissue Engineering of Microvascular Prostheses

Vindigni¹,

Abatangelo²,

Bassetto³

2011

Biomaterials Science and Engineering

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“…4 Current synthetic, small diameter (<6 mm) coronary grafts frequently become occluded due to early thrombosis (blood clots) and late intimal hyperplasia (thickening of the inner wall of the vessel). 5 To prevent thrombosis and hyperplasia, a human endothelial layer can be formed on the luminal surface of the synthetic graft via inoculation of endothelial cells. 6 To possess the proper strength of adhesion and function, these cells must be grown on a substrate that inherently favors adhesion, organization and specific cell-cell communication.…”

Section: Introductionmentioning

confidence: 99%

Electrospun vascular graft properties following femtosecond laser ablation

Lee

Lim

Powell

et al. 2011

J of Applied Polymer Sci

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For polymers in tissue engineering to reach their full potential, the three-dimensional integration of cells with scaffolds must become more complex. In vascular grafts this is particularly challenging as specific physical property requirements must be met. We apply a combination of polymer processing techniques-electrospinning and femtosecond laser ablation-to produce microchannels inside the walls of electrospun tubes providing for spatially-controlled cell seeding. To determine if such a scaffold can provide the desired physical properties, a greater understanding of the relationship between manufacturing and mechanics is needed. As the strength of these scaffolds is an important functional component, the relative properties of single, bi-and tri-layer combinations produced using different solvents were compared.The effect of fiber layer thickness was also investigated. The thickness of the hexafluorisopropanol-derived layer dominated the overall scaffold properties regardless of the nature of the other two layers. Although laser-machined microchannels had substantial effects on single layer and some of the bilayers, in the ''final'' trilayer scaffold, little effect on mechanical properties was observed. The concept of ''vascular wall engineering'' could successfully produce trilayer composite scaffolds that maintain the targeted mechanical properties while allowing intricate, three-dimensional cell seeding.

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Enhanced intimal thickening of expanded polytetrafluoroethylene grafts coated with fibrin or fibrin-releasing vascular endothelial growth factor in the pig carotid artery interposition model

Cited by 60 publications

References 28 publications

Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

New Developments in Tissue Engineering of Microvascular Prostheses

Electrospun vascular graft properties following femtosecond laser ablation

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