Enhanced insecticidal activity of Bacillus thuringiensis using a late embryogenesis abundant peptide co-expression system

Intermittent induction of LEA peptide by lactose enhances the expression of insecticidal proteins in Bacillus thuringiensis

“…1). The results have already been described in our previous study [9], where the cell growth pattern of the transformants and nontransformants was mostly similar.…”

“…The strains and the protocol for the construction of the expression vector are detailed in our previous article [ 9 ].…”

“…The protocol for the insertion of the LEA peptide gene into the pHT01 vector has been detailed in our previous article [ 9 ].…”

“…Our group has attempted to increase the expression level of insecticidal proteins by using Bt as an expression host and the LEA peptide coexpression method [9]. In this method, LEA peptides are expressed in Bt Abbreviations Bt, Bacillus thuringiensis; E. coli, Eschericia coli; IPTG, isopropyl b-D-thiogalactopyranoside; LEA, late embryogenesis abundant; SDS/PAGE, sodium dodecyl sulfate/polyacrylamide gel electrophoresis; TSB, tryptic soy broth.…”

“…Our group has attempted to increase the expression level of insecticidal proteins by using Bt as an expression host and the LEA peptide coexpression method [ 9 ]. In this method, LEA peptides are expressed in Bt under the control of the lac promoter, increasing the insecticidal protein expression.…”