Enhanced Agrobacterium‐mediated transformation revealed attenuation of exogenous plasmid DNA installation in recipient bacteria by exonuclease VII and SbcCD

Abstract

Horizontal DNA transfer is one of the major driving forces in bacterial evolution. Genes from other bacteria are beneficial from ecological and evolutionary viewpoints, that is, foreign nucleotide sequences could provide new functions and enable the cell to survive in new environments (Ochman et al. 2000). However, in many cases, foreign DNAs contain genes that encode something hazardous, such as toxins, and the addition of foreign nucleotide sequences disturbs currently working genetic systems (Kobayashi 2004; Hall et al. 2017; Baltrus 2013). Restriction endonucleases are firewalls against the entry of foreign DNAs, such as phage DNAs (Luria & Human 1952; Bertani & Weigle 1953; Tock & Dryden 2005). In addition, nucleases other than restriction enzymes are likely to play roles in the degradation of foreign DNAs (Roer et al. 2015).

Isolation and Analysis of Donor Chromosomal Genes Whose Deficiency Is Responsible for Accelerating Bacterial and Trans-Kingdom Conjugations by IncP1 T4SS Machinery

“…Further characterization in terms of possible gene interaction within the chromosomal mutants based on physiological analysis with the possible regulators within the IncP1 plasmids will lead for better understanding of the isolated genes’ diversity with the TKC mechanism. Additionally, it will be interesting to determine the specificity of T4SS-mediated IncQ conjugal transfer in the isolated mutant strains by using Ti plasmid (VirB/D4-T4SS system) in comparison to IncP1-type system as it has been reported to serve as a delivery system in Agrobacterium ( Bohne et al, 1998 ; Ohmine et al, 2018 ; Kiyokawa et al, 2020 ).…”