Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction

Zhong, Shigen; Shu, Shiyu; Wang, Zhigang; Luo, Jinyong; Zhong, Weiying; Ran, Haitao; Zheng, Yuanyi; Yin, Yuehui; Ling, Zhiyu

doi:10.1016/j.ultras.2011.08.013

Cited by 38 publications

(42 citation statements)

References 24 publications

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“…17,18 To date, UTMD has been successfully used for gene transfection in diseases of the heart, lung, brain, uterus, pancreas, kidneys, retina, skin, and testes. [19][20][21][22][23][24][25][26][27] To the best of our knowledge, very few studies have investigated the use of US-mediated gene therapy for SCI. Thus, it is still unknown whether USmediated gene transfection represents an effective treatment strategy for SCI.…”

mentioning

confidence: 99%

Nerve growth factor delivery by ultrasound-mediated nanobubble destruction as a treatment for acute spinal cord injury in rats

Song¹,

Wang

Shen³

et al. 2017

IJN

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Background Spinal cord injuries (SCIs) can cause severe disability or death. Treatment options include surgical intervention, drug therapy, and stem cell transplantation. However, the efficacy of these methods for functional recovery remains unsatisfactory. Purpose This study was conducted to explore the effect of ultrasound (US)-mediated destruction of poly(lactic-co-glycolic acid) (PLGA) nanobubbles (NBs) expressing nerve growth factor (NGF) ( NGF /PLGA NBs) on nerve regeneration in rats following SCI. Materials and methods Adult male Sprague Dawley rats were randomly divided into four treatment groups after Allen hit models of SCI were established. The groups were normal saline (NS) group, NGF and NBs group, NGF and US group, and NGF /PLGA NBs and US group. Histological changes after SCI were observed by hematoxylin and eosin staining. Neuron viability was determined by Nissl staining. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining was used to examine cell apoptosis. NGF gene and protein expressions were detected by quantitative reverse transcription polymerase chain reaction and Western blotting. Green fluorescent protein expression in the spinal cord was examined using an inverted fluorescence microscope. The recovery of neural function was determined using the Basso, Beattie, and Bresnahan test. Results NGF therapy using US-mediated NGF /PLGA NBs destruction significantly increased NGF expression, attenuated histological injury, decreased neuron loss, inhibited neuronal apoptosis in injured spinal cords, and increased BBB scores in rats with SCI. Conclusion US-mediated NGF /PLGA NBs destruction effectively transfects the NGF gene into target tissues and has a significant effect on the injured spinal cord. The combination of US irradiation and gene therapy through NGF /PLGA NBs holds great promise for the future of nanomedicine and the development of noninvasive treatment options for SCI and other diseases.

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confidence: 99%

Nerve growth factor delivery by ultrasound-mediated nanobubble destruction as a treatment for acute spinal cord injury in rats

Song¹,

Wang

Shen³

et al. 2017

IJN

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“…Zhong transplanted DAPI-labeled MSCs to the myocardial infarction models by means of UTMD, and found that the expression of various cytokines in UTMD-treated animals was much higher than that observed in non-treated controls. They concluded that UTMD changes the myocardial microenvironment and promotes the homing of MSCs to the ischemic myocardium [97]. Xu established the targeted delivery of MSCs into the myocardium by using diagnostic US irradiating lipid-coated MB destruction along with bone-marrow-derived MSC infusion.…”

Section: Stem Cell Transplantationmentioning

confidence: 99%

Advanced Microbubbles as a Multifunctional Platform Combining Imaging and Therapy

Wang

et al. 2016

Applied Sciences

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Microbubbles as traditional ultrasound contrast agents have seen tremendous developments and bio-applications in the past decades. Due to their outstanding performance, advanced microbubbles as a multifunctional platform combining both imaging and therapy have been increasingly attracting attention. Associated with ultrasound-mediated stimuli, targeting drug transportation with high precision can be established and, as a consequence, a synergistic treatment strategy may prevail, which implies a bright perspective for this brand-new technology. This perspective article will summarize the latest developments on the advanced microbubbles, and review their emerging biomedical applications for the vast community of both applied ultrasound and functional ultrasound-based materials.

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“…94,194,207 Many TERM applications have a low percentage of SC retention and increasing retention would improve therapies. Localized cell delivery can occur by acoustic manipulation, in which cells coated with functionalized MBs are pushed to desired sites by US waves.…”

Section: Perfluorocarbon Particlesmentioning

confidence: 99%

Monitoring/Imaging and Regenerative Agents for Enhancing Tissue Engineering Characterization and Therapies

et al. 2015

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The past three decades have seen numerous advances in tissue engineering and regenerative medicine (TERM) therapies. However, despite the successes there is still much to be done before TERM therapies become commonplace in clinic. One of the main obstacles is the lack of knowledge regarding complex tissue engineering processes. Imaging strategies, in conjunction with exogenous contrast agents, can aid in this endeavor by assessing in vivo therapeutic progress. The ability to uncover real-time treatment progress will help shed light on the complex tissue engineering processes and lead to development of improved, adaptive treatments. More importantly, the utilized exogenous contrast agents can double as therapeutic agents. Proper use of these Monitoring/Imaging and Regenerative Agents (MIRAs) can help increase TERM therapy successes and allow for clinical translation. While other fields have exploited similar particles for combining diagnostics and therapy, MIRA research is still in its beginning stages with much of the current research being focused on imaging or therapeutic applications, separately. Advancing MIRA research will have numerous impacts on achieving clinical translations of TERM therapies. Therefore, it is our goal to highlight current MIRA progress and suggest future research that can lead to effective TERM treatments.

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Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction

Cited by 38 publications

References 24 publications

Nerve growth factor delivery by ultrasound-mediated nanobubble destruction as a treatment for acute spinal cord injury in rats

Nerve growth factor delivery by ultrasound-mediated nanobubble destruction as a treatment for acute spinal cord injury in rats

Advanced Microbubbles as a Multifunctional Platform Combining Imaging and Therapy

Monitoring/Imaging and Regenerative Agents for Enhancing Tissue Engineering Characterization and Therapies

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