Enhanced Hemocompatibility of Lysine Grafted Polyacrylonitrile Electrospun Nanofiber Membranes as a Potential Bilirubin Adsorption in Hemoperfusion

Abstract

Hyperbilirubinemia is one of most severe clinical diseases, which is caused by the accumulation of unconjugated bilirubin. Electrospun nanofiber membranes used as highly efficient bilirubin adsorbents have been applied to remove the extra bilirubin in hemoperfusion for their high surface area and easy functionalized properties. In this work, Lysine (Lys) grafted polyacrylonitrile (PAN) electrospun nanofiber membranes doped with organic Hectorite (OHec) (Lys-HPAN@OHec) have been fabricated via a series of modified process, including pore forming, alkaline hydrolysis and grafting reaction. The obtained Lys-HPAN@OHec nanofiber membranes have been analyzed in detail and investigated the adsorption capacity for bilirubin. Compared with original PNA membranes, Lys-HPAN@OHec nanofiber membranes show an excellent bilirubin adsorption capacity and more stable rejection rate of bovine serum albumin (BSA). The maximum adsorption capacity of Lys-HPAN@OHec membranes for bilirubin is 64 mg/g, the adsorption process of Lys-HPAN@OHec membranes matched the Langmuir model well. In addition, dynamic adsorption reveals that the adsorption equilibrium time of Lys-HPAN@OHec membranes is about 2 h. Significantly, Lys-HPAN@OHec membranes have excellent biocompatibility and hemocompatibility. This study demonstrates that the novel Lys-HPAN@OHec membranes may provide a new way to treat hyperbilirubinemia.

Theory-Driven Tailoring of the Microenvironment of Quaternary Ammonium Binding Sites on Electrospun Nanofibers for Efficient Bilirubin Removal in Hemoperfusion

“…Liu and co-workers grafted lysine on polyacrylonitrile electrospun nanofibers. The maximum adsorption capacity toward bilirubin was 64.0 mg g −1 [ 16 ]. Studies have found that necessary modifications need to be carried out on electrospun fibers before their usage as bilirubin adsorbents.…”

Metal–organic framework (UiO-66 and UiO-66-NH2)-based adsorbents for bilirubin removal used in hemoperfusion

“…Hemodialysis has a poor removal effect on albumin bound bilirubin, and HP is the most effective method for treating hyperbilirubinemia. 6,7…”

“…Hemodialysis has a poor removal effect on albumin bound bilirubin, and HP is the most effective method for treating hyperbilirubinemia. 6,7 The adsorbent in the HP column determines the effectiveness of bilirubin removal, so the development of safe and efficient bilirubin adsorbents is of great signicance. 8 Traditional bilirubin adsorbents such as activated carbon, 9 adsorption resin, 10 polymer beads 11 and bilirubin imprinted polymers 12 and novel bilirubin adsorbents based on nanomaterials including aerogels, 13,14 MOFs, 15,16 nanober materials, 3,17 MXene 18,19 have been continuously reported and innovated for many years to improve adsorption efficiency.…”