Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase

Durall, Claudia; Rukminasari, Nita; Lindblad, Peter

doi:10.1016/j.algal.2016.03.027

Cited by 12 publications

(12 citation statements)

References 40 publications

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“…3). In addition, an enhanced growth was recently observed at low light intensity (3 µmol photons m -2 s -1 ) when overexpressing native phosphenolpyruvate carboxylase in Synechocystis (Durall et al, 2016). Clearly, RuBisCO is one of the limiting steps in the CBB cycle under high light, but not under low light conditions in Synechocystis.…”

Section: Discussionmentioning

confidence: 94%

Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Liang

Lindblad

2016

Metabolic Engineering

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Section: Discussionmentioning

confidence: 94%

Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Liang

Lindblad

2016

Metabolic Engineering

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“…101 Although the whole cycle has not been tested experimentally yet, it has been reported that overexpressing PEPc in Synechocystis PCC 6803 enhanced cell growth under low light conditions. 106 There is another synthetic carbon xation cycle, crotonylcoenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle, which has been examined in vitro. 107 The CETCH cycle uses enoyl-CoA carboxylases/reductases, which are not present in autotrophic CO 2 xing organisms, to carboxylate CO 2 .…”

Section: Alternative Carbon Fixation Cyclesmentioning

confidence: 99%

Engineering photoautotrophic carbon fixation for enhanced growth and productivity

Liang¹,

Lindberg²,

Lindblad³

2018

Sustainable Energy Fuels

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“…As a first step towards implementing these pathways in Synechocystis, we overexpressed the native pepc and the three first enzymes (phosphoenolpyruvate synthase (PPSA), PEPc and malate dehydrogenase), which are all native in Synechocystis. Interestingly, the engineered strain overexpressing pepc showed a higher PEPc level, increased chlorophyll a content and increased in vitro PEPc activity [20].…”

Section: Introductionmentioning

confidence: 98%

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Durall

Lindberg

et al. 2020

Biotechnol Biofuels

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Background: Cyanobacteria can be metabolically engineered to convert CO 2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. Results: The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium Synechocystis PCC 6803 with increased phosphoenolpyruvate carboxylase (PEPc) levels. The resulting engineered strain (CD-P) showed significantly increased ethylene production (10.5 ± 3.1 µg mL −1 OD −1 day −1) compared to the control strain (6.4 ± 1.4 µg mL −1 OD −1 day −1). Interestingly, extra copies of the native pepc or the heterologous expression of PEPc from the cyanobacterium Synechococcus PCC 7002 (Synechococcus) in the CD-P, increased ethylene production (19.2 ± 1.3 and 18.3 ± 3.3 µg mL −1 OD −1 day −1 , respectively) when the cells were treated with the acetyl-CoA carboxylase inhibitor, cycloxydim. A heterologous expression of phosphoenolpyruvate synthase (PPSA) from Synechococcus in the CD-P also increased ethylene production (16.77 ± 4.48 µg mL −1 OD −1 day −1) showing differences in the regulation of the native and the PPSA from Synechococcus in Synechocystis. Conclusions: This work demonstrates that genetic rewiring of cyanobacterial central carbon metabolism can enhance carbon supply to the TCA cycle and thereby further increase ethylene production.

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Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase

Cited by 12 publications

References 40 publications

Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Engineering photoautotrophic carbon fixation for enhanced growth and productivity

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

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