Enhanced gene expression through temperature profile‐induced variations in molecular architecture of thermoresponsive polymer vectors

Lavigne, Matthieu D.; Pennadam, Sivanand S.; Ellis, J. S.; Yates, Laura L.; Alexander, Cameron; Górecki, Dariusz C.

doi:10.1002/jgm.992

Cited by 40 publications

(34 citation statements)

References 39 publications

(47 reference statements)

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“…However, the transfection result of P(NIPAAm-co-NDAPM)-b-PEI/DNA complexes is partially different from that of the previously studied thermoresponsive complexes at two different temperature programs (13,28). The difference was attributed to the different transfection methods.…”

Section: Synthesis Of P(nipaam-co-ndapm)-b-peicontrasting

confidence: 65%

Novel Thermoresponsive Nonviral Gene Vector: P(NIPAAm-co-NDAPM)-b-PEI with Adjustable Gene Transfection Efficiency

et al. 2008

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A thermoresponsive cationic copolymer, poly( N-isopropylacrylamide- co- N-(3-(dimethylamino)propyl)methacrylamide)- b-polyethyleneimine (P(NIPAAm- co-NDAPM)- b-PEI), was designed and synthesized as a potential nonviral gene vector. The lower critical solution temperature (LCST) of P(NIPAAm- co-NDAPM)- b-PEI in water measured by UV-vis spectroscopy was 38 degrees C. P(NIPAAm- co-NDAPM)- b-PEI as the gene vector was evaluated in terms of cytotoxicity, buffer capability determined by acid-base titration, DNA binding capability characterized by agarose gel electrophoresis and particle size analysis, and in vitro gene transfection. P(NIPAAm- co-NDAPM)- b-PEI copolymer exhibited lower cytotoxicity in comparison with 25 kDa PEI. Gel retardation assay study indicated that the copolymer was able to bind DNA completely at N/P ratios higher than 30. At 27 degrees C, the mean particle sizes of P(NIPAAm- co-NDAPM)- b-PEI/DNA complexes decreased from 1200 to 570 nm corresponding to the increase in N/P ratios from 10 to 60. When the temperature changed to 37 degrees C, the mean particle sizes of complexes decreased from 850 to 450 nm correspondingly within the same N/P ratio range due to the collapse of thermoresponsive PNIPAAm segments. It was found that the transfection efficiency of P(NIPAAm- co-NDAPM)- b-PEI/DNA complexes was higher than or comparable to that of 25 kDa PEI/DNA complexes at their optimal N/P ratios. Importantly, the transfection efficiency of P(NIPAAm- co-NDAPM)- b-PEI/DNA complexes could be adjusted by altering the transfection and cell culture temperature.

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Section: Synthesis Of P(nipaam-co-ndapm)-b-peicontrasting

confidence: 65%

Novel Thermoresponsive Nonviral Gene Vector: P(NIPAAm-co-NDAPM)-b-PEI with Adjustable Gene Transfection Efficiency

et al. 2008

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“…In gene delivery studies where thermoresponsive polymers were used the temperature at which one or two of the aforementioned steps were performed at was changed. In particular, in studies where polyethyleneimmine (PEI) with grafted PNIPAAm [43], chitosan grafted with PNIPAAm [44], linear and branched NIPAAm, DMAEMA and PEI polymers [2] and PEG polymers with grafted PEI chains [45] were used, the complexation and transfection temperature were changed to enhance the transfection efficiency. In other studies only the incubation or complexation temperature were varied using random terpolymers of P(NIPAAm-co-DMAEMA-co-BuMA) [18,46] or PNIPAAm copolymers [47], while both complexation and incubation temperature were varied using a polyarginine polymer conjugated with PNIPAAm [48].…”

Section: Gene Deliverymentioning

confidence: 99%

Thermoresponsive Polymers for Biomedical Applications

2011

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Thermoresponsive polymers are a class of "smart" materials that have the ability to respond to a change in temperature; a property that makes them useful materials in a wide range of applications and consequently attracts much scientific interest. This review focuses mainly on the studies published over the last 10 years on the synthesis and use of thermoresponsive polymers for biomedical applications including drug delivery, tissue engineering and gene delivery. A summary of the main applications is given following the different studies on thermoresponsive polymers which are categorized based on their 3-dimensional structure; hydrogels, interpenetrating networks, micelles, crosslinked micelles, polymersomes, films and particles.

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“…Kept below this temperature, responsive polymers show a relaxed, hydrophilic, extended chain conformation, thus allowing the polyplex to dissociate [139]. A widely used temperature-sensitive polymer is poly-N-isopropylacrylamide (pNIPAM) [140][141][142][143][144][145]. Its LCST of 32-33 C is close to physiological temperature but can be shifted by incorporation of hydrophobic or hydrophilic groups [138,146].…”

Section: Thermosensitive Polymersmentioning

confidence: 99%

Chemically Programmed Polymers for Targeted DNA and siRNA Transfection

Salcher

Wagner

2010

Topics in Current Chemistry

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Plasmid DNA and siRNA have a large potential for use as therapeutic nucleic acids in medicine. The way to the target cell and its proper compartment is full of obstacles. Polymeric carriers help to overcome the encountered barriers. Cationic polymers can interact with the nucleic acid in a nondamaging way but still require optimization with regard to transfer efficiency and biocompatibility. Aiming at viruslike features, as viruses are the most efficient natural gene carriers, the design of bioresponsive polymers shows promising results regarding DNA and siRNA delivery. By specific chemical modifications dynamic structures are created, programmed to respond towards changing demands on the delivery pathway by cleavage of labile bonds or conformational changes, thus enhancing biocompatible gene delivery.

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Enhanced gene expression through temperature profile‐induced variations in molecular architecture of thermoresponsive polymer vectors

Cited by 40 publications

References 39 publications

Novel Thermoresponsive Nonviral Gene Vector: P(NIPAAm-co-NDAPM)-b-PEI with Adjustable Gene Transfection Efficiency

Novel Thermoresponsive Nonviral Gene Vector: P(NIPAAm-co-NDAPM)-b-PEI with Adjustable Gene Transfection Efficiency

Thermoresponsive Polymers for Biomedical Applications

Chemically Programmed Polymers for Targeted DNA and siRNA Transfection

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