Enhanced gastric residence time of acyclovir by floating raft formulation using box-behnken design

Munusamy, Rajalakshmi; Shanmugasundharam, Sangeetha

doi:10.1016/j.heliyon.2024.e24301

Cited by 1 publication

(1 citation statement)

References 27 publications

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“…A similar diluent was utilized to prepare the stock solution for the acyclovirloaded floating raft formulation. 17,18 Sample Preparation Male albino rabbits were utilised to spike plasma. The IAEC of the SRM College of Pharmacy at SRMIST, Kattankulathur, Chennai, India, approved the experimental protocol (IAEC/271/2021).…”

Section: Preparation Of Stock Solutionmentioning

confidence: 99%

Green Analytical HPLC Method Development of Acyclovir Loaded Floating Raft Formulation in Spiked Rabbit Plasma

Munusamy,

Hepzhibah,

Shanmugasundharam

2024

IJDDT

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Objective: The need of this investigation is to design eco-friendly, sensitive, and precise bioanalytical high-performance liquid chromatography (HPLC) techniques that leverage green analytical tools such as green analytical procedure index (GAPI), analytical eco scale (AES), and analytical greenness metric (AGREE) to analyze ACV-spiked rabbit plasma and ACV-loaded floating raft formulation (FRF) because The literature survey revealed insufficient data for the HPLC approach to FRF. Method: Methods involving plasma spikes mirroring the in-vivo models provide insights into potential real-life scenarios and the functionality of floating raft formulations loaded with ACV. The Design Expert tool optimized critical process parameters and identified significant factors influencing the chromatographic technique. Results: The CCD was used to optimize the flow rate (0.6 mL/min) and buffer solvent ratio (99:1) for separation. The C18 column (250 X 4.6 mm) was utilized to achieve the elution of ACV. The developed method was thoroughly validated through ICH guidelines. The ACV, lower limit of detection (LLoD), and lower limit of quantitation (LLoQ) retention times were established at 2.3 minutes, 15, and 20 ng/mL, respectively. Discussion: In summary, the study demonstrated the exceptional performance sensitivity, specificity, and robustness of the bioanalytical method. The method proved highly effective in quantifying ACV in plasma samples in terms of revalidatability, transferability, reproducibility, and the product development cycle. The developed method proves that using both design of experiments (DoE) and green analytical chemistry together creates a valid, eco-friendly, and reliable way to analyze the making of an ACV-loaded floating raft formulation.

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Section: Preparation Of Stock Solutionmentioning

confidence: 99%