Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis

Tan, Giin-Yu Amy; Chen, Chia-Lung; Ge, Liya; Li, Ling; Wang, Lin; Zhao, Lei; Yu, Mo; Tan, Swee Ngin; Wang, Jing‐Yuan

doi:10.1016/j.jbiosc.2013.08.020

Cited by 45 publications

(25 citation statements)

References 17 publications

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“…Figure 4 shows representative GC-MS chromatograms of PHA monomers [37]. A recent study by Tan et al [132] showed that PHA monomers with carbon number between 4 and 16 have strong linear correlations with their respective retention times and response factors (R 2 ≥ 0.987) under GC-MS, which enabled the retention time and response factor of other PHA monomers to be predicted. This method allowed a wide series of PHA, ranging from scl-PHA to lcl-PHA, to be reliably detected and quantified in the absence of reference standards.…”

Section: Quantification and Characterization Of Pha In Biomassmentioning

confidence: 95%

“…Despite their advantages, chromatography-based methods require PHA to be depolymerized and chemically converted into acids, diols or methyl ester derivatives prior to analysis [105,130,132]. This meant that chromatography-based methods are unable to distinguish if different PHA monomers are part of several homogenous PHA polymers or if they are part of a single PHA copolymer.…”

Section: Quantification and Characterization Of Pha In Biomassmentioning

confidence: 99%

“…For chromatography-based methods, the analysis of PHA polymer is similar to that for intracellular PHA, requiring depolymerization of the polymer, usually combined with chemical derivatization before it can be analyzed [43,130,132], which meant that chromatography methods cannot analyze PHA as an intact polymer. NMR on the other hand, could study the chemical makeup of an intact PHA polymer and differentiate between PHA blends and PHA copolymer though providing details on the topology and functional group in molecules [150,153,154,168].…”

Section: Monomeric Composition and Distributionmentioning

confidence: 99%

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Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

et al. 2014

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Section: Quantification and Characterization Of Pha In Biomassmentioning

confidence: 95%

Section: Quantification and Characterization Of Pha In Biomassmentioning

confidence: 99%

Section: Monomeric Composition and Distributionmentioning

confidence: 99%

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Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

et al. 2014

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“…The cell dry mass (CDM) of the freeze-dried biomass was determined. The PHA content was quantified by subjecting dried biomass (5 to 10 mg) to methanolysis followed by a GC-MS analysis, at a split ratio of 50:1, according to the procedures described by Tan et al (39). …”

Section: Methodsmentioning

confidence: 99%

“…The PHA polymer (5 to 10 mg) was derivatized according to the procedures described by Tan et al (39) for GC-MS analysis. In the 1 H-NMR and 13 C-NMR analyses, 30 mg of the PHA polymer was dissolved in 0.7 mL deuterated chloroform containing 0.03% (v/v) tetramethylsilane (TMS).…”

Section: Methodsmentioning

confidence: 99%

Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain <i>Pseudomonas putida</i> NBUS12

Tan

Chen

et al. 2015

Microbes and environments

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Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer medium-chain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a genetically-distinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L−1 and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6 ), 3-hydroxyoctanoate (C8 ), 3-hydroxydecanoate (C10 ), 3-hydroxydodecanoate (C12 ), and 3-hydroxytetradecanoate (C14 ) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.

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Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis

Cited by 45 publications

References 17 publications

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain <i>Pseudomonas putida</i> NBUS12

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

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