Enhanced functional DNA biosensor for distance-based read-by-eye quantification of various analytes based on starch-hydrolysis-adjusted wettability change in paper devices

Chen, Yijing; Zhang, Lang; Huang, Jinkun; Deng, Zihao; Yuan, Yali; Zou, Jianmei; Nie, Jinfang; Zhang, Yun

doi:10.1039/d0ra04619a

Cited by 10 publications

(3 citation statements)

References 52 publications

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“…The hydrophilic paper surface displayed the lowest blue channel intensity, indicating a lower colorimetric sensitivity. This difference can be attributed to the high wettability of the hydrophilic surface, causing the reaction product to spread and disperse over a larger area [ 35 ], resulting in a lower intensity. Furthermore, the hydrophobic paper surface exhibited a relatively higher blue channel intensity compared to the hydrophilic paper devices, indicating a moderate enhancement in colorimetric sensitivity (Fig.…”

Section: Resultsmentioning

confidence: 99%

Tuning Hydrophobicity of Paper Substrates for Effective Colorimetric detection of Glucose and Nucleic acids

Sudarsan,

Shetty,

Chinnappan

et al. 2023

Anal Bioanal Chem

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This study investigated the colorimetric response of standard glucose, serum glucose, and nucleic acid assays on various paper surfaces with different wettability, including hydrophilic, hydrophobic, and nearly superhydrophobic surfaces. Water contact angles (WCA) formed by water droplets on each surface were measured using ImageJ software. The hydrophilic surface showed no contact angle, while the hydrophobic and nearly superhydrophobic surfaces exhibited contact angles of 115.667° and 133.933°, respectively. The colorimetric sensitivity of the standard glucose assay was analyzed on these surfaces, revealing enhanced sensitivity on the nearly superhydrophobic surface due to the high molecular crowding effect owing to its non-wetting behavior and eventually confined reaction product at the sample loading zone. The hydrophobic nature of the surface restricts the spreading and diffusion of the reaction product, leading to a controlled and localized concentration of the assay product leading to moderate colorimetric intensity. On the other hand, the hydrophilic surface showed the least enhancement in colorimetric sensitivity; this is attributed to the high wettability of the hydrophilic surface causing the reaction product to spread extensively, resulting in a larger area of dispersion and consequently a lower colorimetric intensity. The measured limit of detection (LOD) for nucleic acid on nearly superhydrophobic surfaces was found to be 16.15 ng/µL, which was almost four-fold lower than on hydrophilic surfaces (60.08 ng/µL). Additionally, the LODs of standard glucose and clinical serum samples were two-fold lower on nearly superhydrophobic surfaces compared to hydrophilic surfaces. Our findings clearly highlight the promising potential of utilizing superhydrophobic surfaces to significantly enhance colorimetric sensitivity in paper-based diagnostic applications. This innovative approach holds promise for advancing point-of-care diagnostics and improving disease detection in resource-limited settings.

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Section: Resultsmentioning

confidence: 99%

Tuning Hydrophobicity of Paper Substrates for Effective Colorimetric detection of Glucose and Nucleic acids

Sudarsan,

Shetty,

Chinnappan

et al. 2023

Anal Bioanal Chem

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“…For the detection of metal ions, the dPADs have also been previously reported using the principle based on the deposition of chemical chelating compounds, such as dithizone [ 30 ] and a synthesized porphyrin [ 31 ]. Interestingly, the growing interest in dPAD application is reflected by the increasing number of publications during recent years, by using several strategies such as G-quadruplex-DNAzyme [ 32 ], starch hydrolysis [ 33 ], ionophore-doped ion-selective nanospheres [ 34 , 35 ], and synthesized fluorescent ligands [ 25 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

A Simple Distance Paper-Based Analytical Device for the Screening of Lead in Food Matrices

et al. 2021

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A simple and rapid distance paper-based analytical device (dPAD) for the detection of lead (Pb) in foods is proposed herein. The assay principle is based on competitive binding between carminic acid (CA) and polyethyleneimine (PEI) to Pb in a food sample. The paper channels were pre-immobilized with PEI, before reacting with a mixture of the sample and CA. Pb can strongly bind to the CA; hence, the length of the red color deposition on the flow channel decreased as a lower amount of free CA bound to PEI. The dPAD exhibited good linear correlation, with ranges of 5–100 µg·mL−1 (R2 = 0.974) of Pb. Although, the limit of detection (LOD) of this platform was rather high, at 12.3 µg·mL−1, a series of standard additions (8.0, 9.0, and 10.0 µg·mL−1) can be used to interpret the cutoff of Pb concentrations at higher or lower than 2 µg·mL−1. The presence of common metal ions such as calcium, magnesium, nickel, and zinc did not interfere with the color distance readout. The validity of the developed dPAD was demonstrated by its applicability to screen the contamination of Pb in century egg samples. The results obtained from the dPAD are in accordance with the concentration measured by atomic absorption spectroscopy (AAS) (n = 9). In conclusion, this proposed dPAD, combined with the standard addition method, could be applied for screening Pb contamination in food matrices. This platform is, therefore, potentially applicable for field measurements of Pb in developing countries, because it is cheap and rapid, and it requires no significant laborious instruments.

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“…For the detection of metal ions, the dPADs have also been previously reported using the principle based on the deposition of chemical chelating compounds, such as dithizone (Cai et al, 2017) and a synthesized porphyrin (Pratiwi et al, 2017). Interestingly, the growing interest in dPAD application is reflected by the increasing number of publications during recent years, by using several strategies such as G-quadruplex-DNAzyme (Wu et al, 2020), starch hydrolysis (Chen et al, 2020), ionophore-doped ion-selective nanospheres (Du et al, 2020;Shibata et al, 2019), and synthesized fluorescent ligands (Nguyen et al, 2020;Ninwong, Sangkaew, et al, 2020).…”

Section: Figure 2 Graphical Abstract Introductionmentioning

confidence: 99%

Development of distance paper-based devices and electrochemical sensors for food and cosmetic safety assessment

Katelakha

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This study aimed to integrate analytical techniques for supporting the safety assessment in food and cosmetics. The two analytical methods were proposed, including i) the development of the paper-based device for the screening detection of lead (Pb) in food matrices, ii) development of the disposable screen-printed electrode for the detection of non-permitted color in halal products. For the first approach, the assay principle was�based on competitive binding between carminic acid (CA) and polyethyleneimine (PEI) to Pb in food samples. The reduction of the PEI-CA color distance is proportional to the concentration of Pb. To categorize food which higher or lower the cut-off of the Pb concentration (2 ?g?mL?1), the standard addition technique combined with the concept of a drawing PAD was integrated using a series of standard addition (8.0, 9.0, and 10.0 ?g?mL?1). The results obtained from the dPAD are in accordance with the concentration measured by the atomic absorption spectroscopy (AAS). The results from the second approach demonstrated that carminic acid and carmoisine were simultaneously assayed under the acidic condition (100 mM sodium citrate buffer, pH 3.0). The electrode was scanned with differential pulse voltammetry (DPV) from ?1.5 V to +1.5 V, and by using a bare unmodified electrode, the detection limit was obtained at 33.68 ?M. The method was obtained with acceptable precision with 2.78? 9.52 %CVs. We demonstrate the proof-of-concept for the detection of carminic acid in lipsticks. The recovery of > 80% was comparable to those obtained by ultra-high performance liquid chromatography (ultra-HPLC). The recovery study of carminic acid in the beverage and raw material (cosmetic grade) ranged from 101.9% ? 123.6% and 93.2% ? 109.0%, respectively. Furthermore, to improve the sensitivity of this technique sufficient to detect the CA fortified in actual food samples, the electrode surface was modified with AuNP/PEI composite. Interestingly, the sensitivity was dramatically 24-times improved comparing to the bare electrode. A linear range of 10 100 ?M (R2�= 0.986) was achieved by using DPV and setting the potential at 0.297 V. In conclusion, an electrochemical method based on a screen-printed electrode modified with AuNP/PEI composite was a promising tool for sensitive detection of carminic acid for assisting the safety monitoring in halal products.

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Enhanced functional DNA biosensor for distance-based read-by-eye quantification of various analytes based on starch-hydrolysis-adjusted wettability change in paper devices

Abstract: A functional DNA sensor was initially developed for the distance-measuring quantification of various analytes based on the starch-hydrolysis-adjusted wettability change of paper.

Cited by 10 publications

References 52 publications

Tuning Hydrophobicity of Paper Substrates for Effective Colorimetric detection of Glucose and Nucleic acids

Tuning Hydrophobicity of Paper Substrates for Effective Colorimetric detection of Glucose and Nucleic acids

A Simple Distance Paper-Based Analytical Device for the Screening of Lead in Food Matrices

Development of distance paper-based devices and electrochemical sensors for food and cosmetic safety assessment

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