Enhanced fluorescence emission of Me-ADOTA+ by self-assembled silver nanoparticles on a gold film

Sørensen, Thomas Just; Laursen, Bo W.; Luchowski, Rafał; Shtoyko, Tanya; Akopova, Irina; Gryczyński, Zygmunt; Gryczyński, Ignacy

doi:10.1016/j.cplett.2009.05.064

Cited by 46 publications

(45 citation statements)

References 29 publications

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“…Recently, a fluorescence enhancement of ~200-fold on colloidal silver nanoparticles was reported; however, only for selected “hot spots” on a gold mirror. 12 It is important to note that AF647-SA is one of the brightest organic fluorophores with a quantum efficiency better than 0.30 and high extinction coefficient of 239 000 M −1 cm −1 , which is spectrally equivalent to widely used Cy 5 fluorophore for biomolecule labeling.…”

Section: Resultsmentioning

confidence: 99%

“…5,12,31 Accordingly, the lifetime measurements provide valuable information about the mechanism of the enhancement. For example, if the intensity is enhanced but the lifetime is not changed, this means that the mechanism of intensity enhancement is due to an enhanced excitation intensity or an increase in the fluorophore concentration.…”

Section: Resultsmentioning

confidence: 99%

“…9 To maximize the enhanced signal from the nanoparticles, in some instances, a combination of solid films with nanoparticles was also used. 10–12 Several excellent review articles describing the studies on the effect of metallic nanostructures on fluorescence were published previously. 13–16 …”

Section: Introductionmentioning

confidence: 99%

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Fabrication and Characterization of Planar Plasmonic Substrates with High Fluorescence Enhancement

2010

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The use of plasmonic nanostructures for fluorescence signal amplification is currently a very active research field. The detection of submonolayers of proteins labeled with organic dyes is a widely used technique in surface-based immunoassays and DNA hybridization. There is a strong interest in the development of new optical and chemical methods to increase the signal from ultralow concentrations of dyes on the surface of sensor substrates. Herein, we have explored the possibility of using vacuum-deposited silver nanostructures on dielectric layers and silver mirrors as potential plasmonic substrates that effectively amplify fluorescence over a broad spectral range. By optimizing deposition parameters for dielectric layers and silver nanostructures and applying thermal annealing processes, we observed large fluorescence amplifications from three different dye-strept(avidin) conjugates: about 7-fold for a UV/blue dye AF350-Av, 49-fold for a blue-green dye AF488-SA, and up to 208-fold for red-emitting AF647-SA dye. The observed amplification factors for the ensemble of fluorophores are very promising for development of surface-based bioassays. These substrates can be prepared using simple vacuum deposition in which we circumvent using the expensive nanofabrication methods. In addition, unlike most nanofabrication methods, the present approach is appropriate for large scale fabrication of substrates with microscope slide surface area suitable for sensing applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Fabrication and Characterization of Planar Plasmonic Substrates with High Fluorescence Enhancement

2010

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“…Sørensen et al found about similar enhancement in hot spots where fractals were deposited on gold mirror and dye was deposited directly on the fractals [25]. Thus, different configuration of PP did not affect the fluorescence enhancements.…”

Section: Discussionmentioning

confidence: 93%

Sandwich type plasmonic platform for MEF using silver fractals

et al. 2015

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In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

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“…The azaoxa-triangulenium class of dyes, 12–14 in the following represented by azadioxatriangulenium (ADOTA + , Figure 1), are bright organic dyes with a long fluorescence lifetime (≈25 ns) and emission in the red. 15, 16 The fluorescence lifetimes are comparable to those of dansyl and pyrene. However, the emission at 550–600 nm results in much reduced interference from protein auto-fluorescence and Rayleigh scattering.…”

Section: Introductionmentioning

confidence: 86%

Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay

Sørensen

Thyrhaug

Szabelski

et al. 2013

Methods Appl. Fluoresc.

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Of the many optical bioassays available, sensing by fluorescence anisotropy have great advantages as it provides a sensitive, instrumentally simple, ratiometric method of detection. However, it is hampered by a severe limitation as the emission lifetime of the label needs to be comparable to the correlation lifetime (tumbling time) of the biomolecule which is labelled. For proteins of moderate size this is in the order of 20–200 ns, which due to practical issues currently limits the choice of labels to the dansyl-type dyes and certain aromatics dyes. These have the significant drawback of UV/blue absorption and emission as well as an often significant solvent sensitivity. Here, we report the synthesis and characterization of a new fluorescent label for high molecular weight biomolecules assay based on the azadioxatriangulenium motif. The NHS ester of the long fluorescence lifetime, red emitting fluorophore: azadioxatriangulenium (ADOTA-NHS) was conjugated to anti-rabbit Immunoglobulin G (antiIgG). The long fluorescence lifetime was exploited to determine the correlation time of the high molecular weight antibody and its complex with rabbit Immuniglobulin G (IgG) with steady-state fluorescence anisotropy and time-resolved methods: solution phase immuno-assay was performed following either steady-state or time-resolved fluorescence anisotropy. By performing a variable temperature experiment it was determined that the binding of the ligand resulted in an increase in correlation time by more than 75 %, and a change in the steady-state anisotropy increase of 18%. The results show that the triangulenium class of dyes can be used in anisotropy assay for detecting binding events involving biomolecules of far larger size than what is possible with the other red emitting organic dyes.

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Enhanced fluorescence emission of Me-ADOTA+ by self-assembled silver nanoparticles on a gold film

Cited by 46 publications

References 29 publications

Fabrication and Characterization of Planar Plasmonic Substrates with High Fluorescence Enhancement

Fabrication and Characterization of Planar Plasmonic Substrates with High Fluorescence Enhancement

Sandwich type plasmonic platform for MEF using silver fractals

Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay

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