Enhanced fluorescence detection of proteins using ZnO nanowires integrated inside microfluidic chips

Guo, Liang; Shi, Yundi; Liu, Xiangfei; Han, Zhitao; Zhao, Zhenjie; Chen, Yong; Xie, Wenhui

doi:10.1016/j.bios.2017.08.003

Cited by 91 publications

(42 citation statements)

References 39 publications

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“…As a signal amplifier, the polypyrrole nanowire based sensor could detect serum C-reactive protein (CRP) levels through monitoring the conductance change [ 143 ]. As is shown in Figure 9(a) , with a larger binding surface area and intrinsic fluorescence enhancement, Guo et al developed a ZnO nanowire FET biosensor to detect both AFP and CEA with varying diameters and lengths of ZnO, in which the ZnO nanowire was successfully fabricated by controlling the content of polyethyleneimine and growth time in microfluidic channels [ 140 ]. Through the detailed research on surface functionalization and covalent immobilization of biomarkers, Janissen et al fabricated an InP nanowire-based FET biosensor.…”

Section: Microstructure-based Biomarker Sensorsmentioning

confidence: 99%

“… Nanowire-based FET biomarker sensor. (a) is a schematic of the ZnO nanowire-based biosensor which was combined with microfluidic technology, reproduced from Guo et al [ 140 ], and (b) is the FET-like biosensor which is fabricated by sputtering and E-beam evaporation with multiple shadow masks, reproduced from Liu et al [ 141 ] with permission. …”

Section: Figurementioning

confidence: 99%

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Recent Progress of Biomarker Detection Sensors

Liu

Cui

2020

Research

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Early cancer diagnosis and treatment are crucial research fields of human health. One method that has proven efficient is biomarker detection which can provide real-time and accurate biological information for early diagnosis. This review presents several biomarker sensors based on electrochemistry, surface plasmon resonance (SPR), nanowires, other nanostructures, and, most recently, metamaterials which have also shown their mechanisms and prospects in application in recent years. Compared with previous reviews, electrochemistry-based biomarker sensors have been classified into three strategies according to their optimizing methods in this review. This makes it more convenient for researchers to find a specific fabrication method to improve the performance of their sensors. Besides that, as microfabrication technologies have improved and novel materials are explored, some novel biomarker sensors—such as nanowire-based and metamaterial-based biomarker sensors—have also been investigated and summarized in this review, which can exhibit ultrahigh resolution, sensitivity, and limit of detection (LoD) in a more complex detection environment. The purpose of this review is to understand the present by reviewing the past. Researchers can break through bottlenecks of existing biomarker sensors by reviewing previous works and finally meet the various complex detection needs for the early diagnosis of human cancer.

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Section: Microstructure-based Biomarker Sensorsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Recent Progress of Biomarker Detection Sensors

Liu

Cui

2020

Research

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“…Fabrication of microfluidic structures is carried out through a wet etching, milling, sanding, and laser techniques. In order to obtain closed canals, substrates need to be bonded either to another glass, silicon (through anodic bonding) [ 108 ], or polymer layer (trough plasma bonding) [ 109 ]. Therefore, implementation of complex spatial structures in glass microsystems is difficult.…”

Section: Technology Of Microfluidic Systems With Fluorescence Detementioning

confidence: 99%

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

Nawrot

Drzozga

Baluta

et al. 2018

Sensors

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The clinical applications of sensing tools (i.e., biosensors) for the monitoring of physiologically important analytes are very common. Nowadays, the biosensors are being increasingly used to detect physiologically important analytes in real biological samples (i.e., blood, plasma, urine, and saliva). This review focuses on biosensors that can be applied to continuous, time-resolved measurements with fluorescence. The material presents the fluorescent biosensors for the detection of neurotransmitters, hormones, and other human metabolites as glucose, lactate or uric acid. The construction of microfluidic devices based on fluorescence uses a variety of materials, fluorescent dyes, types of detectors, excitation sources, optical filters, and geometrical systems. Due to their small size, these devices can perform a full analysis. Microfluidics-based technologies have shown promising applications in several of the main laboratory techniques, including blood chemistries, immunoassays, nucleic-acid amplification tests. Of the all technologies that are used to manufacture microfluidic systems, the LTCC technique seems to be an interesting alternative. It allows easy integration of electronic and microfluidic components on a single ceramic substrate. Moreover, the LTCC material is biologically and chemically inert, and is resistant to high temperature and pressure. The combination of all these features makes the LTCC technology particularly useful for implementation of fluorescence-based detection in the ceramic microfluidic systems.

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“…It's well known that ZnO has been approved by the U.S. Food and Drug Administration (FDA) as a material with good biocompatibility [ 16 ]. Therefore, ZnO also has extensive application prospects in biomedical field, including drug delivery [ [17] , [18] ], [[, 18 ] imaging [ 19 , 20 ], implant materials [ 21 , 22 ] and biosensors [ 23 , 24 ]. Photocatalytic performance, as one of the research hotspots of ZnO, has laid a solid foundation for its application in environment and energy fields, but its application potential in the field of biomedicine has not been fully explored.…”

Section: Introductionmentioning

confidence: 99%

High special surface area and “warm light” responsive ZnO: Synthesis mechanism, application and optimization

Miao

Liu

et al. 2022

Bioactive Materials

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In this study, a new series of zinc oxide (ZnO) with high specific surface area and narrow energy band gap are prepared using a facile microwave-induced method. The corresponding formation mechanism is also discussed for the first time. Due to the introduction of C, these ZnO can be excited by long wave temperature light without harmful short wave radiation, and play an efficient photocatalytic activity. This valuable property fundamentally improves the biological safety of its photocatalytic application. Herein, taking teeth whitening as an example, the photocatalytic performance of ZnO is evaluated. The “pure” yellow light-emitting diode (PYLED) with high biological safety is used as the excitation source. It is found that this method could effectively remove pigment on the tooth surface through physical adsorption. In addition, these ZnO could generate active oxygen to degrade the pigment on the tooth surface under the irradiation of yellow light. Some further optimization of these “warm light” responsive ZnO is also discussed in this systematical study, which could open up new opportunities in biomedical field.

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Enhanced fluorescence detection of proteins using ZnO nanowires integrated inside microfluidic chips

Cited by 91 publications

References 39 publications

Recent Progress of Biomarker Detection Sensors

Recent Progress of Biomarker Detection Sensors

A Fluorescent Biosensors for Detection Vital Body Fluids’ Agents

High special surface area and “warm light” responsive ZnO: Synthesis mechanism, application and optimization

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