Enhanced Fenton Reaction for Xenobiotic Compounds and Lignin Degradation Fueled by Quinone Redox Cycling by Lytic Polysaccharide Monooxygenases

Abstract

The Fenton reaction is considered to be of great significance in the initial attack of lignocellulose in wood-decaying fungi. Quinone redox cycling is the main way to induce the Fenton reaction in fungi. We show that lytic polysaccharide monooxygenases (LPMOs), through LPMO-catalyzed oxidation of hydroquinone, can efficiently cooperate with glucose dehydrogenase (GDH) to achieve quinone redox cycling. The LPMO/GDH system can enhance Fe 3+ -reducing activity, H 2 O 2 production, and hydroxyl radical generation, resulting in a fueled Fenton reaction. The system-generated hydroxyl radicals exhibited a strong capacity to decolorize different synthetic dyes and degrade lignin. Our results reveal a potentially critical connection between LPMOs and the Fenton reaction, suggesting that LPMOs could be involved in xenobiotic compound and lignin degradation in fungi. This new role of LPMOs may be exploited for application in biorefineries.

Elaboration of a Phytoremediation Strategy for Successful and Sustainable Rehabilitation of Disturbed and Degraded Land

“…Additionally, Actinobacteria are considered among the main drivers of lignin breakdown and humification during compost production, a process seemingly dependent upon enhanced LAC expression and activity and formation of quinone-like substances [137,138]. In addition, a lytic polysaccharide monooxygenase-glucose dehydrogenase (LPMO/GDH) system has recently been implicated in lignin degradation and quinone redox cycling and appears to do so by increasing Fe 3 + -reducing activity, H 2 O 2 production, and hydroxyl radical generation by an enhanced Fenton process [139]. These observations indicate a role for both LPMO and LAC in lignin breakdown and turnover which, together with HS processing by saprophytic fungi and some members of the Ascomycota [140], suggests that specific microbial consortia may indeed drive humification.…”

A mechanistic study on electro-Fenton system cooperating with phangerochate chrysosporium to degrade lignin

“…31,34 (3) As P. chrysosporium grew at 4 V electrical potential, its secreted organic acids lowered the pH of the environment, and together with Fe 2+ in the system, the generated H 2 O 2 constituted an electro-Fenton reaction, generating ROSs to act indiscriminately on the lignin functional group. 44 (4) Low molecular weight compounds with reducing ability secreted by P. chrysosporium, such as organic acids, fatty acids, Fe 3+ chelators and catechol derivatives, can reduce Fe 3+ to Fe 2+ and carry out a cyclic synergistic oxidation of lignin. 45,46 The mechanism is shown in Fig.…”

Application of Causality Modelling for Prediction of Molecular Properties for Textile Dyes Degradation by LPMO

“…The widespread occurrence in fungal genomes points to its crucial role in biomass degradation [ 10 ]. However, up to now, only one research by Fei et al from 2021 pointed to dye decolorization by the LPMO/GDH-induced Fenton system [ 11 ]. The results indicate that the addition of LPMO could significantly enhance the degradation of dyes, due to the enhanced level of hydroxyl radicals achieved by LPMO.…”

“…It has to be emphasized the hydroquinone was found as metabolites of tested dyes. Its important property is that it is an electronic transmitter, by which it can further participate in the LPMO-induced Fenton reaction [ 11 ]. Ikram et al evaluated the degradation potential of eleven bacterial strains for azo dye methyl red.…”

“…Notably, hydroquinone was found among the metabolites of tested dyes. As an electronic transmitter, hydroquinone can further participate in the LPMO-induced Fenton reaction, which could be the reason for the higher decolorization rate of disazo and triphenylmethane [ 11 ].…”