“…The noteworthy outcomes revealed that, after fine-tuning the gene expression regulator, signal peptide promoter sequences, and the ribosome binding site (RBS) upstream of the amyZ1 gene, an astonishing enzyme activity of 2.6 times greater than that of the native enzyme was achieved, totaling an impressive 4824.2 U/mL. Moreover, through a rigorous optimization process for the carbon and nitrogen sources, a remarkable α-amylase activity of 49,082.1 U/mL was attained within a 3 L fermenter [ 37 ].…”