Enhanced expression of α1-acid glycoprotein and fucosylation in hepatitis B patients provides an insight into pathogenesis

Abstract

Altered glycosylation and concentration of alpha1-acid glycoprotein has been known to be related to the pathogenesis of the hepatic diseases. The present study investigated enhanced fucosylation of AGP in the sera of chronic hepatitis B (HBV-CH) and hepatitis B cirrhosis (HBV-LC) patients by high performance anion exchange chromatography and by ELISA using fucose binding Aleuria aurantia lectin. The concentration of AGP determined by ELISA using monoclonal anti-human AGP (mAb-AGP) showed high level of AGP in HBV-CH and HBV-LC patients. This was further judged by association constant (K (A)) measured by surface plasmon resonance analysis. There was no apparent linkage variation of sialic acid among different patient groups when tested with two sialic acid binding lectins viz., Maackia amurensis agglutinin (MAA, NeuAc alpha2-3-) and Sambucus nigra agglutinin (SNA, NeuAc alpha2-6-) respectively. There was no change of oligosaccharide branching in HBV-CH in comparison to controls whereas a slight change was observed in HBV-LC using ConA. The above results suggest that the changes in concentration of AGP and fucosylation have a prognostic value of hepatitis diseases and it could be possible to use AGP as diagnostic marker besides clinical examination and routine laboratory investigation.

Altered glycosylation and expression of plasma alpha-1-acid glycoprotein and haptoglobin in rheumatoid arthritis

“…The binding of Con-A with AGP and Hp was studied using lectin ELISA [2]. Statistical analysis was made by two tail t-test and Mann-Whitney test.…”

“…The clinical relevance of glycosylation in various physiological processes and diseases has been understood by sugar profiling. Aberrant glycosylation patterns have been recognised and well studied in several chronic diseases [1][2][3][4][5][6]. Advancements of glycan analysis tools have helped in deciphering the sugar code which is proved to be indispensible in identifying the disease mechanism as well as for the development of novel diagnostic or prognostic markers [7,8].…”

Enhanced expression and fucosylation of ficolin3 in plasma of RA patients

“…In this study, we have observed the increased fucosylation in ficolin 3 in plasma of RA patients. Elevated levels of fucose in many serum proteins have been assessed in various diseases like hepatocellular carcinoma (HCC) [11], RA [12], pancreatic cancer [13] and hepatitis B [14]. The increase in fucose level may not be limited to ficolin 3 but in other serum proteins as well.…”

Alterations of glycan branching and differential expression of sialic acid on alpha fetoprotein among hepatitis patients

“…Fucosylation of AFP is also considered to be a most important biomarker in accurate diagnosis of liver diseases. There are number of reports of changes in the fucosylation of other serum glycoproteins in liver diseases namely Tf, AGP [10] and haptoglobin. In our study high amount of fucose content was found in all patients' groups; of them HCC showed highest and HBV-CH lowest amount of fucose.…”

“…Glycosylation change in patients' glycoproteins such as fucosylation in alpha-1 acid glycoprotein (AGP) in ascetic fluid with liver cirrhosis [4,5], haptoglobin (Hp) in alcoholic liver disease [6,7], serum cholinesterase in LC [8,9]. Previously, we observed that enhanced fucosylation of AGP in chronic hepatitis B (HBV-CH) and hepatitis B cirrhosis (HBV-LC) patients [10]. Alpha-fetoprotein (AFP), which is an embryospecific glycoprotein composed of approximately 580 amino acid (MW~72 kDa) and containing 3-5% of carbohydrate [11][12][13].…”