Enhanced expression of TACE contributes to elevated levels of sVCAM-1 in endometriosis

Proestling, Katharina; Yotova, Iveta; Gamperl, Susanne; Hauser, Christoph; Wenzl, René; Schneeberger, Christian; Szabo, Ladislaus; Mairhofer, Mario; Husslein, H; Kuessel, Lorenz

doi:10.1093/molehr/gay042

Cited by 1 publication

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“…Slides were counterstained in hematoxylin before they were dehydrated and mounted. Scoring and immunohistochemical analysis was performed, as described previously [ 56 ] and as outlined in the figure legends.…”

Section: Methodsmentioning

confidence: 99%

MLLT11 Regulates Endometrial Stroma Cell Adhesion, Proliferation and Survival in Ectopic Lesions of Women with Advanced Endometriosis

Proestling,

Husslein,

Hudson

et al. 2023

IJMS

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MLLT11 is a gene implicated in cell differentiation and the development and progression of human cancers, but whose role in the pathogenesis of endometriosis is still unknown. Using quantitative RT-PCR and immunohistochemistry, we analyzed 37 women with and 33 women without endometriosis for differences in MLLT11 expression. We found that MLLT11 is reduced in the ectopic stroma cells of women with advanced stage endometriosis compared to women without endometriosis. MLLT11 knockdown in control stroma cells resulted in the downregulation of their proliferation accompanied by G1 cell arrest and an increase in the expression of p21 and p27. Furthermore, the knockdown of MLLT11 was associated with increased apoptosis resistance to camptothecin associated with changes in BCL2/BAX signaling. Finally, MLLT11 siRNA knockdown in the control primary stroma cells led to an increase in cell adhesion associated with the transcriptional activation of ACTA2 and TGFB2. We found that the cellular phenotype of MLLT11 knockdown cells resembled the phenotype of the primary endometriosis stroma cells of the lesion, where the levels of MLLT11 are significantly reduced compared to the eutopic stroma cells of women without the disease. Overall, our results indicate that MLLT11 may be a new clinically relevant player in the pathogenesis of endometriosis.

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Section: Methodsmentioning

confidence: 99%