Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome

Wairich, Andriele; Vitali, Ariane; Adamski, Janete Mariza; Lopes, Karina Letícia; Duarte, Guilherme Coutinho Kullmann; Ponte, Lucas Roani; Costa, Henrique Keller; Menguer, Paloma Koprovski; Santos, Rinaldo Pires dos; Fett, Janette Palma; Sperotto, Raul Antônio; Ricachenevsky, Felipe Klein

doi:10.1590/1678-4685-gmb-2022-0190

Cited by 6 publications

(13 citation statements)

References 62 publications

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“…Transcripts of OsZIP2 and OsZIP3 have been postulated to exist in Zn-deficient root tissues . The increase of OsZIP1–3 expression that would have been predicted after 2 weeks of Zn deficiency in plants was not seen . OsZIP4 expression is increased when soil zinc levels are low.…”

Section: Approaches For Zn Biofortification In Ricementioning

confidence: 94%

“…94 The increase of OsZIP1−3 expression that would have been predicted after 2 weeks of Zn deficiency in plants was not seen. 95 OsZIP4 expression is increased when soil zinc levels are low. Root apical meristem and vascular bundle phloem cells express OsZIP4 in Zndeficient plants.…”

Section: Molecular and Genetic Approaches For Zinc Fortifications 323...mentioning

confidence: 99%

“…The abundance of OsZIP4 transcripts has been shown to be very stable over long periods of time. 95 Data from microarray studies and laser microdissection of the rice root (RiceXPro;http://ricexpro.dna.affrc.gojp/) have shown regional differences in OsZIP gene expression. Root tissues (epidermis, exodermis, and sclerenchyma) were discovered to express the corresponding genes, namely, OsZIP5, OsZIP7, and OsZIP8 (Figure 2).…”

Section: Molecular and Genetic Approaches For Zinc Fortifications 323...mentioning

confidence: 99%

“…OsZIP8 expression was shown to be lower between days 27 and 76 post infection (DAI), but the reverse trend was seen between DAI and post-treatment days (DAT). The abundance of OsZIP4 transcripts has been shown to be very stable over long periods of time . Data from microarray studies and laser microdissection of the rice root (RiceXPro;) have shown regional differences in OsZIP gene expression.…”

Section: Approaches For Zn Biofortification In Ricementioning

confidence: 99%

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Zinc-Fortified Rice: Approach to Improve Dietary Zinc Consumption and Effect on Human Health

Zubair,

Zaib,

Ul Ghani

et al. 2023

ACS Agric. Sci. Technol.

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Micronutrient deficiencies are a key limiting factor associated with malnutrition in more than half of the human population worldwide. Micronutrient deficiencies, particularly zinc (Zn) deficiency, are the most prominent in children and pregnant women. To reduce the prevalence of these deficiencies, several different approaches were adopted including food fortification, supplementation, dietary diversification, and biofortification. Rice (Oryza sativa) is a staple food crop worldwide, with long-term sustainability and cost-effectiveness of biofortification comparative to other approaches, makes rice biofortification an interesting target to address micronutrient deficiencies issues. Therefore, about 1.5 million farming households are growing numerous Zn-biofortified rice varieties, indicating that biofortification programs are a reliable approach in various countries. In this review we discussed different strategies to improve consumption and the use of potential Zn biofortification strategies in rice, including the advantage of crop for biofortification, addresses different challenges, and proposed solutions to significantly improve Zn-biofortification program. Plant stem and root cells rely heavily on the OsZIP1 family of zinc finger proteins for zinc transport and distribution. This family includes OsZIP3, OsZIP4, OsZIP5, and OsZIP8. Despite its importance, very few studies have looked into how zinc is transported and used in plant and germline tissues. Attempts to increase grain zinc levels through agronomic treatments have been shown to not always be successful but have a higher chance of success when combined with genetic engineering. Marker-assisted selection might take advantage of the discovered grain Zn main impact and consistent quantitative trait loci (QTLs).

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Section: Approaches For Zn Biofortification In Ricementioning

confidence: 94%

Section: Molecular and Genetic Approaches For Zinc Fortifications 323...mentioning

confidence: 99%

Section: Molecular and Genetic Approaches For Zinc Fortifications 323...mentioning

confidence: 99%

Section: Approaches For Zn Biofortification In Ricementioning

confidence: 99%

See 2 more Smart Citations

Zinc-Fortified Rice: Approach to Improve Dietary Zinc Consumption and Effect on Human Health

Zubair,

Zaib,

Ul Ghani

et al. 2023

ACS Agric. Sci. Technol.

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“…S6A). Recent studies have indicated that overexpression of the OsNAC5 gene enhances the translocation of Fe and Zn from vegetative tissues to seeds (Wairich et al, 2023).…”

Section: Knockout Of Osnac5 Reduced CD Accumulation In Rice Grainmentioning

confidence: 99%

The Transcription Factor OsNAC5 Mediates Cadmium Stress Response and Accumulation in Rice

Hu,

Chen,

Wang

et al. 2024

Preprint

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Cadmium (Cd) is a toxic heavy metal that endangers human health via contaminated rice consumption. Reduction Cd uptake in rice is essential for minimizing grain Cd accumulation. However, the transcriptional regulatory of Cd uptake in rices remains largely elusive. This study reveals that the transcription factor OsNAC5 in Oryza sativa positively regulates the Cd transporter gene OsNRAMP1, influencing Cd uptake. OsNAC5 is primarily expressed in roots, localized to the nucleus and is upregulated by Cd induced H2O2. OsNAC5 knockout reduces Cd levels in both shoots and roots and increases Cd sensitivity. OsNRAMP1 expression, enhanced by Cd stress, dependents on OsNAC5, which binds to CATGTG motifs in the OsNRAMP1 promoters activating its expression. Loss of OsNAC5 function decreases Cd accumulation in rice grains. Our findings elucidate the transcriptional regulation of Cd stress response in rice and suggest biotechnological approaches to reduce Cd uptake in crops.

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Wheat NAC transcription factor NAC5‐1 is a positive regulator of senescence

Evans,

Mogg,

Soraru

et al. 2024

Plant Direct

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Wheat (Triticum aestivum L.) is an important source of both calories and protein in global diets, but there is a trade‐off between grain yield and protein content. The timing of leaf senescence could mediate this trade‐off as it is associated with both declines in photosynthesis and nitrogen remobilization from leaves to grain. NAC transcription factors play key roles in regulating senescence timing. In rice, OsNAC5 expression is correlated with increased protein content and upregulated in senescing leaves, but the role of the wheat ortholog in senescence had not been characterized. We verified that NAC5‐1 is the ortholog of OsNAC5 and that it is expressed in senescing flag leaves in wheat. To characterize NAC5‐1, we combined missense mutations in NAC5‐A1 and NAC5‐B1 from a TILLING mutant population and overexpressed NAC5‐A1 in wheat. Mutation in NAC5‐1 was associated with delayed onset of flag leaf senescence, while overexpression of NAC5‐A1 was associated with slightly earlier onset of leaf senescence. DAP‐seq was performed to locate transcription factor binding sites of NAC5‐1. Analysis of DAP‐seq and comparison with other studies identified putative downstream target genes of NAC5‐1 which could be associated with senescence. This work showed that NAC5‐1 is a positive transcriptional regulator of leaf senescence in wheat. Further research is needed to test the effect of NAC5‐1 on yield and protein content in field trials, to assess the potential to exploit this senescence regulator to develop high‐yielding wheat while maintaining grain protein content.

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Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome

Cited by 6 publications

References 62 publications

Zinc-Fortified Rice: Approach to Improve Dietary Zinc Consumption and Effect on Human Health

Zinc-Fortified Rice: Approach to Improve Dietary Zinc Consumption and Effect on Human Health

The Transcription Factor OsNAC5 Mediates Cadmium Stress Response and Accumulation in Rice

Wheat NAC transcription factor NAC5‐1 is a positive regulator of senescence

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