Enhanced Expression and Primary Purification of Soluble HBD3 Fusion Protein in Escherichia coli

Abstract

To obtain in-depth information on the overall metabolic behavior of the new good xylitol producer Debaryomyces hansenii UFV-170, batch bioconversions were carried out using semisynthetic media with compositions simulating those of typical acidic hemicellulose hydrolysates of sugarcane bagasse. For this purpose, we used media containing glucose (4.3-6.5 g/L), xylose (60.1-92.1 g/L), or arabinose (5.9-9.2 g/L), or binary or ternary mixtures of them in either the presence or absence of typical inhibitors of acidic hydrolysates, such as furfural (1.0-5.0 g/L), hydroxymethylfurfural (0.01- 0.30 g/L), acetic acid (0.5-3.0 g/L), and vanillin (0.5-3.0 g/L). D. hansenii exhibited a good tolerance to high sugar concentrations as well as to the presence of inhibiting compounds in the fermentation media. It was able to produce xylitol only from xylose, arabitol from arabinose, and no glucitol from glucose. Arabinose metabolization was incomplete, while ethanol was mainly produced from glucose and, to a lesser less extent, from xylose and arabinose. The results suggest potential application of this strain in xyloseto- xylitol bioconversion from complex xylose media from lignocellulosic materials.

High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli

“…The foreign protein could not be expressed fully when the IPTG concentration was too low, also the high IPTG concentration would be harmful to cell growth [18]. In this work, IPTG concentration was examined from 0.1 to 1.0 mM.…”

Human Neutrophil Peptide-1 (HNP-1): A New Anti-Leishmanial Drug Candidate

“…They are classified as α-, β- and θ-defensins [10] where the first two are the most common human antimicrobial peptides. The α-defensins found in neutrophils (polymorphonuclear cells, PMNs) are called human neutrophil peptides (HNP)-1, -2, -3 and -4 [11].…”

Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity

“…The bacteria were harvested and collected by centrifuging at 4000 rpm for 20 min. The purified GST-HBD3 recombinant protein was obtained as previously reported (Huang et al 2007 ).…”

“…Hence, HBD3 protein maintaining a natural physiological concentration which is not efficiently to resist the high-density bacteria invasion within a short time. Prokaryotic expression of HBD3 fusion protein was initially designed and optimized by Huang et al ( 2006 , 2007 ). However, the existence of His tag reduced the production of HBD3.…”