Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis

Huang, Chiung-Fang; Lin, Ting-Hsiang; Guo, Gia‐Luen; Hwang, Wen-Song

doi:10.1016/j.biortech.2009.02.064

Cited by 135 publications

(65 citation statements)

References 29 publications

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“…Apparently, the tested concentration levels of HMF and furfural were not affecting the fermentation and growth of S. stipitis CBS6054. Yeasts including S. stipitis can metabolize furfural to furfuryl alcohol and the enzyme NADH-dependent yeast alcohol dehydrogenase (ADH) is responsible for the reduction (Huang et al 2009). In the present investigation, HMF and furfural were completely metabolized by the strain before significant utilization of sugars started.…”

Section: Discussionmentioning

confidence: 99%

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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Sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. This stresses the importance of utilizing both C 6 and C 5 sugars for conversion into ethanol in order to improve the process economics. During processing of the hydrolysate degradation products such as acetate, 5-hydroxymethylfurfural (HMF) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. In the current study, conversion of both glucose and xylose sugars into ethanol in wet exploded bagasse hydrolysates was investigated without detoxification using Scheffersomyces (Pichia) stipitis CBS6054, a native xylose utilizing yeast strain. The sugar utilization ratio and ethanol yield (Y p/s ) ranged from 88-100% and 0.33-0.41 ± 0.02 g/g, respectively, in all the hydrolysates tested. Hydrolysate after wet explosion at 185°C and 6 bar O 2 , composed of mixed sugars (glucose and xylose) and inhibitors such as acetate, HMF and furfural at concentrations of 3.2 ± 0.1, 0.4 and 0.5 g/l, respectively, exhibited highest cell growth rate of 0.079 g/l/h and an ethanol yield of 0.39 ± 0.02 g/g sugar converted. Scheffersomyces stipitis exhibited prolonged fermentation time on bagasse hydrolysate after wet explosion at 200°C and 6 bar O 2 where the inhibitors concentration was further increased. Nonetheless, ethanol was produced up to 18.7 ± 1.1 g/l resulting in a yield of 0.38 ± 0.02 g/g after 82 h of fermentation.

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Section: Discussionmentioning

confidence: 99%

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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“…These are the potential toxic compounds which inhibit microbial growth and lead to a low yield of ethanol during the prehydrolyzate fermentation. Over the years, different methods have been developed to overcome the inhibition effect of microbial growth which includes, ion exchange chromatography (Chandel et al 2007), a prior adaptation of microorganisms to prehydrolyzate (Huang et al 2009) and genetic modifications in microorganisms through UV mutations (Rakesh et al 2012). These methods are tedious and proper skills are required for development.…”

Section: Introductionmentioning

confidence: 99%

Dilute acid pretreatment of sorghum biomass to maximize the hemicellulose hydrolysis with minimized levels of fermentative inhibitors for bioethanol production

et al. 2017

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Conversion of lignocellulosic biomass into monomeric carbohydrates is economically beneficial and suitable for sustainable production of biofuels. Hydrolysis of lignocellulosic biomass using high acid concentration results in decomposition of sugars into fermentative inhibitors. Thus, the main aim of this work was to investigate the optimum hydrolysis conditions for sorghum brown midrib IS11861 biomass to maximize the pentose sugars yield with minimized levels of fermentative inhibitors at low acid concentrations. Process parameters investigated include sulfuric acid concentration (0.2-1 M), reaction time (30-120 min) and temperature (80-121°C). At the optimum condition (0.2 M sulfuric acid, 121°C and 120 min), 97.6% of hemicellulose was converted into xylobiose (18.02 mg/g), xylose (225.2 mg/g), arabinose (20.2 mg/g) with low concentration of furfural (4.6 mg/g). Furthermore, the process parameters were statistically optimized using response surface methodology based on central composite design. Due to the presence of low concentration of fermentative inhibitors, 78.6 and 82.8% of theoretical ethanol yield were attained during the fermentation of non-detoxified and detoxified hydrolyzates, respectively, using Pichia stipitis 3498 wild strain, in a techno-economical way.

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“…The results obtained in experiments 1 and 2 are comparable with data reported for the conversion of non-detoxifi ed hemicellulose hydrolyzates containing xylose. For comparative purposes, Table 5 summarizes results reported for the fermentation of raw hydrolyzates in related studies (van Zyl et al 1991 ;Amartey and Jeffries 1996;Nigam 2001bNigam , 2002S á nchez et al 2004 ;Huang et al 2009 ;Kurian et al 2010 ).…”

Section: Fermentation Of Raw Hydrolyzatesmentioning

confidence: 99%

“…Following this idea, preliminary fermentation trials were carried out with adapted cells based on media made with neutralized hydrolyzates containing 50 g xylose l -1 and 11.9 g acetic acid l -1 ; however, no signifi cant xylose consumption was observed (data not shown). This fact was ascribed to possible osmotic problems and, in particular, to the high level of acetic acid, whose detrimental effects on the P. stipitis metabolism are well known (Palmqvist and H ä hn-Hagerdal 2000 ;Klinke et al 2004 ;Almeida et al 2007 ;Huang et al 2009 ). Acetic acid inhibition at concentrations about 2 -5 g l -1 have been reported to be inhibitory to osmotolerant yeasts (van Zyl et al 1991 ).…”

Section: Fermentation Of Raw Hydrolyzatesmentioning

confidence: 99%

“…To limit the inhibition caused by the non-saccharide components of the hydrolyzate, fermentations were carried out with adapted strains, as this approach may be advantageous for ethanol production (Agbogbo et al 2008 ;Huang et al 2009 ). Pichia stipitis was able to grow in agar plates made with hydrolyzates containing 2.3 g acetic acid l -1 .…”

Section: Fermentation Of Raw Hydrolyzatesmentioning

confidence: 99%

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Extracting value-added products before pulping: Hemicellulosic ethanol from Eucalyptus globulus wood

Rodriguez‐Lopez

Romaní²,

González‐Muñoz³

et al. 2012

Holzforschung

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Xylose solutions have been produced from Eucalyptus globulus wood by autohydrolysis (with hot, compressed water) and post-hydrolysis (in presence of sulfuric acid). This two-stage process led to solids enriched in cellulose and lignin (suitable as a substrate for pulping) and liquors containing xylose as the major component. The liquid phase from post-hydrolysis also contained other sugars (glucose, arabinose) and acetic acid. Neutralized liquors (as obtained, or after membrane concentration), were employed (directly or after detoxifi cation by ion exchange) as fermentation media for the production of hemicelluosic bioethanol with the yeast Pichia stipitis CECT 1922T. Under the best conditions assayed (fermentation of neutralized, concentrated and detoxifi ed two-stage hydrolysis liquors), bioconversion took place at nearly stoichiometric yield, with a volumetric productivity of 0.37 g l -1 h -1 .

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Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis

Cited by 135 publications

References 29 publications

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Dilute acid pretreatment of sorghum biomass to maximize the hemicellulose hydrolysis with minimized levels of fermentative inhibitors for bioethanol production

Extracting value-added products before pulping: Hemicellulosic ethanol from Eucalyptus globulus wood

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