Enhanced epicurzerenone production via in vitro elicitation of microrhizomes of Curcuma caesia Roxb.

Anjum, Afreen; Quraishi, Afaque

doi:10.1007/s11627-023-10390-0

Cited by 1 publication

(3 citation statements)

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“…In vitro induction of small storage organs has been reported in several plant species [16,28,44]. Microrhizome development is a complex process influenced by numerous internal and external factors [44].…”

Section: Discussionmentioning

confidence: 99%

“…Microrhizomes are miniature rhizomes induced in vitro. Over the past decades, microrhizome induction has been achieved in several rhizomatous crop species, such as Curcuma aromatica [14,15], Curcuma caesia [16], and Kaempferia parviflora [17]. Microrhizome technology is becoming increasingly popular in rhizomatous crops due to its several potential advantages over traditional in vitro culture systems.…”

Section: Introductionmentioning

confidence: 99%

“…Microrhizomes could successfully sprout both in vitro and in vivo [18] and were easily stored and transported [19]. Recently, it has been reported that in vitro-induced microrhizomes could be used for germplasm conservation [20], artificial seed production [21], and secondary metabolite production [16,22]. In ginger, attempts have been made to induce microrhizomes in vitro using high concentrations of sucrose and different combinations of plant growth regulators (PGRs) [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Microrhizome Production, Genetic Homogeneity Assessment, and Field Performance Evaluation in Ginger

Yu,

Hu,

Liu

et al. 2024

Agronomy

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In vitro-induced microrhizomes are promising for producing disease-free planting materials in ginger (Zingiber officinale Rosc.), spice and medicinal crops threatened by several soil-borne diseases. The study examined microrhizome induction, genetic homogeneity, and field performance in ginger. The condition combination of 3.0 mg·L-1 6-benzylaminopurine (BAP), 100 g·L–1 sucrose, and a 12-h photoperiod (the optimal conditions) produced the largest number of microrhizomes among all treatments but resulted in a lower average fresh weight during the 60-day culture period. Larger microrhizomes exhibited greater tolerance to water loss and a higher percentage of sprouting. Therefore, additional efforts were made to increase the size of the microrhizomes. Under the optimal conditions, the fresh weight increased significantly to 280.3 mg and 403.4 mg after 30 days of additional culture and in vitro culture of small-sized microrhizomes, respectively. Flow cytometry analysis and SSR characterization confirmed the genetic homogeneity of the regenerated plants with microrhizomes (MR) and those obtained by directly sowing sprouted microrhizomes into the substrate without acclimatization (FMR). At harvest, the MR had the most robust growth, a significantly higher fresh rhizome weight (206.1 g per plant) than the FMR (121.8 g per plant) and conventional tissue-cultured plants (TC), and similar rhizome finger size (11.5 g and 10.2 cm2) to the FMR. These findings suggest that both the MR and the FMR have advantages over the TC in producing seedling rhizomes of ginger in the first growing season. The established approach may be useful for large-scale production of disease-free ginger rhizomes.

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