Enhanced enzymatic saccharification of sugarcane bagasse pretreated by sodium methoxide with glycerol

Lv, Xiaojing; Lin, Jianghai; Luo, Liang; Zhang, Dou; Lei, Senlin; Xiao, Wenjuan; Xu, Yuan; Yang, Gong; Liu, Zehuan

doi:10.1016/j.biortech.2017.09.137

Cited by 36 publications

(39 citation statements)

References 31 publications

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“…The intensities (I002) of the amorphous cellulose peak and crystalline cellulose peak were considered to calculate the Crystallinity Index (CrI) of all ve samples of bagasse. The CrI of untreated bagasse was 49.67%, which was close to a previously available report [46,43]. The CrI of acid and alkali pretreated sugarcane bagasse was comparatively lower than untreated sugarcane bagasse showing the sequential increment in cellulose content in these samples (Fig.…”

Section: Discussionsupporting

confidence: 88%

“…3b-e). Several workers have been reported similar observation for un-treated and treated bagasse [46,43]. Sugarcane bagasse residue from alkali-pretreatment (10% NaOH at 121 °C for 60 min) was the most severely disrupted followed by 5% NaOH alkali, 10% H 2 SO 4 and 5% H 2 SO 4 acidpretreatments.…”

Section: Discussionsupporting

confidence: 59%

“…Further, cellulignin when pretreated with alkali pretreatment (5% and 10% NaOH at 121 °C for 60 min) showed lower CrI (41.1 and 11.2%) because of the removal of lignin, and thus increased the cellulose concentration in bagasse than that of untreated bagasse and cellulignin. In other words, this sharp decrease in crystallinity due to the alkali pretreatment con rms that the regenerated products were highly amorphous and thus, cellulose surface accessibility and consequently the e ciency of enzymatic hydrolysis were considerably increased [12,45,41,43]. Figures (3a) clearly indicates that the untreated bagasse had highly compact, ordered and rigid bril morphology [32] when compare with acid and alkali pretreated bagasse samples ( Fig.…”

Section: Discussionmentioning

confidence: 81%

“…The peaks at 1,324, 1,514 and 1604 cm − 1 were indicators of hemicelluloses and lignin characteristic [41,43]. More speci cally, 1,324 cm − 1 peak reveals the aromatic hydroxyl groups generated by the cleavage of ether bonds within lignin, 1,514 cm − 1 is associated with the aromatic skeletal modes of lignin whereas 1,604 cm − 1 is stated to be stretching of the C = C and C = O lignin aromatic ring [44,14,43,38]. As observed in Fig.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Tiwari¹,

Yadav²,

Gaur³

et al. 2020

Preprint

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Background: Enhancement of cellulase and xylanase production and improvement of more proficient lignocellulose-degrading enzymes are essential in order to decrease the price of enzymes required in the biomass-to-bioethanol production. Results: The effectiveness of different concentration of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of cellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% cellulose, 25.63% hemicelluloses, 30.36% lignin, 1.44% ash and 2.90% other extractives. Afterthat, The sugarcane bagasse was pretreated with two different concentrations (5% and 10%) of H2SO4 and NaOH at 121°C for 60 min. Among them, the best result was obtained when sugarcane bagasse was pretreated with 10% NaOH solution followed by 10% H2SO4, 5% NaOH and 5% H2SO4 solution. The highest cellulose saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75mg/g, 301.25 mg/g and 276.6 mg/g from 10% H2SO4, 5% NaOH and 5% H2SO4, respectively, which were 55.1, 32.0, 27.1 and 20.6 times higher than the control. Moreover, the FTIR, XRD and SEM analysis showed significant molecule and surface structure changes of the sugarcane bagasse after different pretreatments. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] was used to hydrolyze the pretreated sugarcane bagasse and the optimal condition was determined to be 30 h of enzymatic reaction with 3:1 ration of enzymes under the temperature of 55°C, pH 5.5, substrate concentration of 3% and Tween-20 0.5%. Conclusion: Enzyme supernatants produced by the mixed culture of Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] on various pretreated sugarcane baggase have good cellulase and xylanase activities, leading to celluloses and Hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Tiwari¹,

Yadav²,

Gaur³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…[4] Although pure glycerol could be widely used in foods, cosmetics, and pharmaceuticals, it is difficult and costly to purify glycerol from crude glycerol, and the surplus amount of crude glycerol has changed from a by-product to a burden of the biodiesel industry with no suitable applications. [5] From the perspective of long-term development and large-scale production, bioconversion of crude glycerol to value-added chemicals is an effective way of producing many products from crude glycerol, including butanol, biohydrogen, citric acid, dihydroxyacetone, xanthan gum, and polyhydroxyalkanoates. [3,[6][7][8] Gynostemma pentaphyllum, as an edible and medicinal herb, has been widely used in beverages, food, and medicines.…”

Section: Introductionmentioning

confidence: 99%

Utilization of glycerol and crude glycerol for polysaccharide production by an endophytic fungus Chaetomium globosum CGMCC 6882

Wang

Tao

Gao

et al. 2019

Preparative Biochemistry and Biotechnology

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Crude glycerol is becoming a financial and environmental liability due to its surplus production from biodiesel industry, and its utilization as a fermentation feedstock for value-added chemicals production has been widely studied. In present work, the capacity of an endophytic fungus, Chaetomium globosum CGMCC 6882, using glycerol and crude glycerol for polysaccharide production was investigated. Results showed that the polysaccharide titers from glucose and glycerol were 1.85 and 3.8 g/L, respectively. Moreover, spore morphology of C. globosum CGMCC 6882 was favorable for polysaccharide production. Meanwhile, impurities in crude glycerol have no effect on polysaccharide production by C. globosum CGMCC 6882. Finally, characteristic results of polysaccharides produced from glucose, glycerol, and crude glycerol have suggested that metabolic flux might be a determinant factor on polysaccharide structure. Taken together, this research provided an innovative approach of utilizing crude glycerol produced from the biodiesel production process.

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Fundamental review of organosolv pretreatment and its challenges in emerging consolidated bioprocessing

Chin

Lim

Pang

et al. 2020

Biofuels Bioprod Bioref

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Lignocellulosic biomass is the most abundant renewable source of bioenergy. However, its intertwined structures prevent the embedded cellulose and hemicellulose from undergoing further chemical transformations. Organosolv pretreatment has been known to be effective in reducing the recalcitrance of lignocellulosic biomass. It possesses advantages in the recovery of components and the recovered components such as cellulose can be converted into bioproducts through consolidated bioprocessing (CBP). The main objective of this paper is to review recent developments in organosolv pretreatment, including a comparison of different organic solvents and their optimum operation conditions in terms of pretreatment performance and efficiency. Conventional solvents were also compared with several emerging solvents to discover more potential utilization of organosolv pretreatment. This paper also reviewed fundamental knowledge regarding organosolv pretreatment such as its mechanism, and the chemical and physical properties of the solvents, which are still rarely discussed in the literature due to a lack of conclusive information. The challenges and way forward for organosolv pretreatment were also analyzed in depth to consider the synergistic advantages after the integration of organosolv pretreatment into CBP. It is believed that, through more fundamental exploration and optimization work, organosolv pretreatment can play a vital role in determining the sustainability of CBP, to pave the way for more efficient bioprocessing in the future. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

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Enhanced enzymatic saccharification of sugarcane bagasse pretreated by sodium methoxide with glycerol

Cited by 36 publications

References 31 publications

Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Utilization of glycerol and crude glycerol for polysaccharide production by an endophytic fungus Chaetomium globosum CGMCC 6882

Fundamental review of organosolv pretreatment and its challenges in emerging consolidated bioprocessing

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