Enhanced enzymatic reaction by aqueous two-phase systems using parallel-laminar flow in a double Y-branched microfluidic device

Meng, Shujuan; Xue, Long-Hui; Xie, Changqing; Bai, Ruixue; Yang, Xin; Qiu, Zhongping; Guo, Tao; Wang, Yaolei; Meng, Tao

doi:10.1016/j.cej.2017.10.085

Cited by 43 publications

(28 citation statements)

References 40 publications

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“…43 They provide a benign environment for the biocatalyst along with the possibility of reducing substrate and/or product inhibition by compartmentalization in the other of the two phases. The industrial use of ATPSs is still hampered by their drawbacks such as slow diffusive mass transfer, long settling time for phase separation, and batch processing, 44 so processing in microfluidic systems present a promising tool for wider use of this green technology. 45 Recently, we reported the use of the microfluidics for the generation of a temperature-dependent aqueous micellar two-phase system (AMTPS) containing a surfactant in the time frame of a few seconds (Figure 2 d).…”

Section: Microreactors With Biocatalysts In the Multi-liquid Phase Symentioning

confidence: 99%

“…The very short residence time in the channel was increased by 4 consecutive reaction cycles, resulting in a 4-fold increase in conversion. 44 A theoretical study of the enzymatic production of cephalexin, an important β-lactam antibiotic, using an ATPS based on PEG and phosphate in a microscale device comprising a thin dialysis membrane that provides flow stabilization and prevents transport of the enzyme and enzymesubstrate complex from the salt phase to the PEG phase. In the synthesis catalyzed by penicillin acylase, the effect of counter-current and co-current arrangements on cephalexin yield in microreactors with parallel flow of ATPS was discussed, as well as the possibility of transport enhancement by a direct-current (DC) electric field applied perpendicular to the interface.…”

Section: Microreactors With a Parallel Flow Of Immiscible Liquids mentioning

confidence: 99%

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Let the Biocatalyst Flow

Žnidaršič–Plazl

2021

ACSi

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Industrial biocatalysis has been identified as one of the key enabling technologies that, together with the transition to continuous processing, offers prospects for the development of cost-efficient manufacturing with high-quality products and low waste generation. This feature article highlights the role of miniaturized flow reactors with free enzymes and cells in the success of this endeavor with recent examples of their use in single or multiphase reactions. Microfluidics-based droplets enable ultrahigh-throughput screening and rapid biocatalytic process development. The use of unique microreactor configurations ensures highly efficient contacting of multiphase systems, resulting in process intensification and avoiding problems encountered in conventional batch processing. Further integration of downstream units offers the possibility of biocatalyst recycling, contributing to the cost-efficiency of the process. The use of environmentally friendly solvents supports effective reaction engineering, and thus paves the way for these highly selective catalysts to drive sustainable production.

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Section: Microreactors With Biocatalysts In the Multi-liquid Phase Symentioning

confidence: 99%

Section: Microreactors With a Parallel Flow Of Immiscible Liquids mentioning

confidence: 99%

Let the Biocatalyst Flow

Žnidaršič–Plazl

2021

ACSi

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“…The width of each phase, i.e., the volume each phase occupies in the channel, can be adjusted by changing the flow rate ratio between the two liquids. Such microsystems are often used for two-phase biocatalysis using immiscible hydrophobic substrates, as they provide advantages like short diffusion paths across the aqueous/non-aqueous interface and easy phase separation at the outlets [ 38 , 39 ].…”

Section: Droplet Generation In Microfluidic Systemsmentioning

confidence: 99%

Two-Phase Biocatalysis in Microfluidic Droplets

et al. 2021

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This Perspective discusses the literature related to two-phase biocatalysis in microfluidic droplets. Enzymes used as catalysts in biocatalysis are generally less stable in organic media than in their native aqueous environments; however, chemical and pharmaceutical compounds are often insoluble in water. The use of aqueous/organic two-phase media provides a solution to this problem and has therefore become standard practice for multiple biotransformations. In batch, two-phase biocatalysis is limited by mass transport, a limitation that can be overcome with the use of microfluidic systems. Although, two-phase biocatalysis in laminar flow systems has been extensively studied, microfluidic droplets have been primarily used for enzyme screening. In this Perspective, we summarize the limited published work on two-phase biocatalysis in microfluidic droplets and discuss the limitations, challenges, and future perspectives of this technology.

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“…Therefore, by replacing Equation (22) in Equations ( 7), ( 9) and ( 13), the dimensionless version of the governing and constitutive equations of Poisson-Boltzmann, momentum and Maxwell is obtained, respectively as follows…”

Section: Dimensionless Mathematical Modelmentioning

confidence: 99%

“…Research progress on electroosmotic flows with single-phase fluids is broad, as shown through the references cited in the previous paragraph and the references contained within them in turn. However, several microdevices applications use parallel multiphase flows for continuous chemical processing in analyses and synthesis [ 20 , 21 ], realizing specific operations as mixing and reaction [ 22 ], phase confluence and separation [ 23 ], solvent extraction [ 24 , 25 ], liquid-liquid extraction [ 26 ], purification [ 27 ] and synthesis of polymer membranes [ 28 ]. These parallel flows are a kind of flow pattern generated by using flow-focusing techniques in microsystems [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Start-Up Electroosmotic Flow of Multi-Layer Immiscible Maxwell Fluids in a Slit Microchannel

et al. 2020

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In this investigation, the transient electroosmotic flow of multi-layer immiscible viscoelastic fluids in a slit microchannel is studied. Through an appropriate combination of the momentum equation with the rheological model for Maxwell fluids, an hyperbolic partial differential equation is obtained and semi-analytically solved by using the Laplace transform method to describe the velocity field. In the solution process, different electrostatic conditions and electro-viscous stresses have to be considered in the liquid-liquid interfaces due to the transported fluids content buffer solutions based on symmetrical electrolytes. By adopting a dimensionless mathematical model for the governing and constitutive equations, certain dimensionless parameters that control the start-up of electroosmotic flow appear, as the viscosity ratios, dielectric permittivity ratios, the density ratios, the relaxation times, the electrokinetic parameters and the potential differences. In the results, it is shown that the velocity exhibits an oscillatory behavior in the transient regime as a consequence of the competition between the viscous and elastic forces; also, the flow field is affected by the electrostatic conditions at the liquid-liquid interfaces, producing steep velocity gradients, and finally, the time to reach the steady-state is strongly dependent on the relaxation times, viscosity ratios and the number of fluid layers.

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Enhanced enzymatic reaction by aqueous two-phase systems using parallel-laminar flow in a double Y-branched microfluidic device

Cited by 43 publications

References 40 publications

Let the Biocatalyst Flow

Let the Biocatalyst Flow

Two-Phase Biocatalysis in Microfluidic Droplets

Start-Up Electroosmotic Flow of Multi-Layer Immiscible Maxwell Fluids in a Slit Microchannel

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