Enhanced enzymatic hydrolysis of sugarcane bagasse with ferric chloride pretreatment and surfactant

Wei

et al. 2018

Biotechnol Biofuels

Self Cite

BackgroundThe recalcitrant structure of lignocellulosic biomass made it challenging for their bioconversion into biofuels and biochemicals. Pretreatment was required to deconstruct the intact structure by the removal of hemicellulose/lignin, improving the cellulose accessibility of enzyme. Combinatorial pretreatments with liquid hot water/H2SO4 and ethanol/NaOH of sugarcane bagasse were developed to improve enzymatic hydrolysis under mild conditions.ResultsAfter one-step 60% ethanol containing 0.5% NaOH pretreatment with solid to liquid ratio of 1/10, the glucose yield after hydrolysis for 72 h with enzyme dosage of 20 FPU/g substrate was enhanced by 41% and 205% compared to that of NaOH or 60% ethanol pretreated solids, respectively. This improvement was correlated with the removal of hemicellulose and lignin. However, using combinatorial pretreatments with 1% H2SO4 followed by 60% ethanol containing 0.5% NaOH, the highest glucose yield with Tween 80 reached 76%, representing 84.5% of theoretical glucose in pretreated substrate. While retaining similar glucose yield, the addition of Tween 80 capacitated either a reduction of enzyme loading by 50% or shortening hydrolysis time to 24 h. However, the enhancement with the addition of Tween 80 decreased as hydrolysis time was extended.ConclusionsThis study demonstrated that a combinatorial pretreatment with 1% H2SO4 followed by 60% ethanol containing 0.5% NaOH had significant effects on improving the enzymatic hydrolysis of sugarcane bagasse. The addition of Tween 80 enabled reducing the enzyme loading or shortening the hydrolysis time. This study provided an economically feasible and mild process for the generation of glucose, which will be subsequently converted to bioethanol and biochemicals.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1313-7) contains supplementary material, which is available to authorized users.

Section: Resultsmentioning

confidence: 99%

Enhancing enzymatic saccharification of sugarcane bagasse by combinatorial pretreatment and Tween 80

Wei

et al. 2018

Biotechnol Biofuels

Self Cite

“…Several studies have reported the enhancement of enzymatic saccharification yields by using various surfactants [38,57,58]. In the present study also, the effect of surfactants on enzymatic hydrolysis was investigated by the addition of Tween 80 (v/v) and PEG 6000 (w/v) in the range of 0.05 to 0.15%.…”

Section: Effects Of Surfactant Addition On Enzymatic Hydrolysismentioning

confidence: 92%

Saccharification and fermentation of pretreated banana leaf waste for ethanol production

Suhag

Kumar²,

Singh

2020

SN Appl. Sci.

The present work reveals the potential applicability of banana leaf waste as feedstock for bioethanol production as very less work has been reported on this feedstock. For achieving the same, the performance of crude cellulases of Aspergillus niger JD-11 is explored for saccharification of untreated and pretreated (alkaline, dilute acid and steam) banana leaf waste. Therefore, to determine the best saccharifying conditions, the effects of various parameters such as enzyme loading (5-15 FPU/g), temperature (40-50 °C), surfactant addition (0.05-0.15% Tween 80 and PEG 6000) and substrate concentrations (2-6%) were studied at 150 rpm and pH 5.0. The crude cellulases yielded maximum reducing sugars (524.83 mg/g) from the dilute acid pretreated banana leaf waste at 45 °C, 15 FPU/g enzyme loading and 2% substrate loading in the presence of 0.15% PEG 6000 (w/v) as surfactant. The fermentation of enzymatic hydrolysate was carried out using initial reducing sugars concentration of 40 g/L in the medium at pH 5.5, 150 rpm and 30 °C for 30 h with the help of Saccharomyces cerevisiae. Ethanol production was estimated at every 6 h and maximum ethanol yield of 0.38 g/g sugar and productivity of 1.28 g/L/h was obtained after 12 h of fermentation of banana leaf waste hydrolysate. Thus, the easily obtained banana leaf waste could be a promising feedstock for bioethanol production.

“…The 30/70 weight percent of PVOH/NCC showed higher degradation rate, which indicated 95% weight loss after 80 hours of insertion in the enzyme media. The NCC modified PVOH films can absorb more of the enzyme solution than pure PVOH because of the -OH groups of NCC that enhanced the absorption rate of enzyme in to the composite films [11]. With the increasing of NCC content in the composite, the enzyme absorption rate also increased.…”

Section: Surface Morphological Studymentioning

confidence: 99%

Preparation and Biodegradation of Nanocellulose Reinforced Polyvinyl Alcohol Blend Films in Bioenvironmental Media

Islam

Proma

Rahman

et al. 2017

CSIJ

Solution casting method was used to prepare nanocellulose reinforced polyvinyl alcohol (PVOH) from Oil palm empty fruit bunches. Different environmental test were used to investigate the biodegradability of the composite in soil and compost as well as in water and acidic solution. The morphology of the composite was investigated by scanning electron microscopy. The composite film with nanocellulose and without nanocellulose were compared, nanocellulose modified PVOH film showed more highly degradable by soil and compost as well as in enzymes. From the biodegradation study it was observed that the rate of degradation of the composite depend on the nanocellulose content. Surface morphology study of the composite exposed the distribution of nanocellulose in the PVOH.