Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica

Lee, Kyoung-Mi; Kalyani, Dayanand; Tiwari, Manish Kumar; Kim, Taesu; Dhiman, Saurabh Sudha; Lee, Jung-Kul; Kim, In-Won

doi:10.1016/j.biortech.2012.07.066

Cited by 96 publications

(43 citation statements)

References 33 publications

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“…The column temperature was maintained at 35°C. A sample volume of 25 lL was injected into the system, and the detection was carried out at a wavelength of 255 nm (Bagewadi et al 2016a;Lee et al 2012). The phenolic compounds in the sample were recognized by matching their retention times with those of authentic standard phenolic compounds like 4-hydroxy-3-methoxybenzoic (vanillic) acid and 4-hydroxy-3,5-dimethoxybenzoic (syringic) acid.…”

Section: Methodsmentioning

confidence: 99%

“…Laccases play a significant role in morphogenesis and pathogenesis. Laccases have been identified to have wide industrial applications such as in lignin degradation, pulp bleaching, decolourization of different azo recalcitrant textile dyes, wastewater treatment, fossil fuel desulfurization, biosolubilization of coal, degradation of herbicides, bioremediation, detoxification of pollutants, synthesis of natural products like pigments and antioxidants, biosensors and immunochemical applications (Arora and Sharma 2010;Kalyani et al 2012;Lee et al 2012;Rico et al 2014). Laccase enzymes are extensively dispersed in environment among the plants, insects, fungus and bacteria (Giardina et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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Purpose Current research focuses on the biological delignification of biomass by microbial laccase which is an environmentally friendly process. Methods Various statistical approaches were designed for optimization of laccase production like Plackett-Burman design as well as response surface methodology (RSM). A laccase mediator system was designed for the delignification of saw dust which was molecularly characterized by high-performance liquid chromatography (HPLC), Fourier transformation infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Results The present study reveals wheat bran is a potential substrate for the production of laccase (63 U/g and 9.6 mg/ g protein) under solid-state fermentation by Trichoderma harzianum strain HZN10. Statistical optimization by RSM using central composite design (CCD) revealed that wheat bran contributed maximally to the overall laccase production followed by yeast extract. Laccase production under optimized conditions yielded 510 U/g with 8.09-fold increase. HPLC peaks representing 4-hydroxy-3-methoxybenzoic (vanillic) acid and 4-hydroxy-3,5-dimethoxybenzoic (syringic) acid showed drastic reduction in laccasetreated saw dust sample indicating the elimination of toxic inhibitors, thereby signifying the detoxification of sample.The laccase-treated saw dust showed 1.6-fold increase in reducing sugars after enzymatic (cellulase) hydrolysis. The FTIR analysis revealed the structural alterations occurring during the delignification process. SEM of biologically treated saw dust revealed the morphological alterations during the delignification process targeting the fiber cell walls rich in lignin. Conclusion The delignification of saw dust was effective by laccase mediator system and was evidenced by HPLC, FTIR and SEM analysis. Hence, laccase can be a powerful tool in biomass to biofuel conversions.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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“…A laccase enzyme can extend its substrate range from phenolic to non-phenolic organic substrates if the laccase is supplemented with a mediator (Gochev and Krastanov 2007;Lee et al 2012). A mediator is generally a small-size compound, able to generate stable radicals during reaction (Cañas and Camarero 2010;Euring et al 2011).…”

Section: Reaction Mechanismmentioning

confidence: 99%

“…Laccase is a specific enzyme that acts on lignin, but laccase supplemented with a mediator can act on a wide range of substrates (phenolic compounds) (Fillat and Roncero 2010;Lee et al 2012). It can oxidize the variety of organic compounds present in plant cell walls, such as lignin, ortho-and para-diphenols, aminophenols, polyphenols, aryl diamines, polyamines, and some inorganic ions .…”

Section: Chemical Modificationmentioning

confidence: 99%

Laccase, an Emerging Tool to Fabricate Green Composites: A Review

Mahmood¹,

Hashim²,

Sulaiman³

et al. 2015

BioResources

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In the last two decades, laccases have received much attention from researchers because of their specific ability to oxidize lignin. This function of laccase is very useful for applications in several biotechnological processes, including delignification in the pulp and paper industry and the detoxification of industrial effluents from the textile and petrochemical industries. This review focuses on laccase-mediated fiberboard synthesis. Growing concerns regarding the emission of formaldehyde from wood composites has prompted industrialists to consider the fabrication of green composites. Laccase-mediated fiber treatments oxidize the lignin component without affecting the cellulose structure. As a result, free radicals are generated on the fiber surface, and these can act as potential reactive sites for further cross-linking reactions in board manufacturing. Binderless fiberboards prepared using such methods can be considered as green composites because the manufacturing process involves no additional resin.

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“…Y. lipolytica has been used to develop a biological detoxification process, due to its natural capability to produce extracellular laccase (Lee et al, 2012). This capability was harnessed to oxidize the lignin-derived phenolic compounds for pretreated rice straw to mitigate their inhibitory impacts on cellulase.…”

Section: Strategies Associated With Extension Of Substrate Rangementioning

confidence: 99%

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

2014

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Today's biotechnologists seek new biocatalysts to meet the growing demand for the bioproducts. This review critically evaluates the potential use of Y. lipolytica as an oleaginous cell factory platform. This yeast has undergone extensive modifications for converting a wide range of hydrophobic and hydrophilic biomass, including alkane, oil, glycerol, and sugars to fatty acid-based products. This article highlights challenges in the development of this platform and provides an overview of strategies to enhance its potential in the sustainable production of biodiesel, functional dietary lipid compounds, and other value-added oleochemical compounds. Future applications of the recombinant Y. lipolytica platform are also discussed.

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Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica

Cited by 96 publications

References 33 publications

Optimization of laccase production and its application in delignification of biomass

Optimization of laccase production and its application in delignification of biomass

Laccase, an Emerging Tool to Fabricate Green Composites: A Review

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

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