Enhanced enrichment and detection of thermotolerant <i>Campylobacter</i> species from water using the Portable Microbe Enrichment Unit and real-time PCR

Pitkänen, Tarja; Bräcker, Juliane BräckerJ.; Miettinen, Ilkka T.; Heitto, Anneli; Pesola, Jouni; Hakalehto, Elias

doi:10.1139/w09-040

Cited by 14 publications

(10 citation statements)

References 26 publications

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“…This specific PMEU approach had been provisionally tested in a water distribution network system in Kuopio City, Finland (with 100.000 inhabitants and 350 km of pipelines) (Hakalehto et al, 2011c). Both hygienic indicators and various pathogens, such as Salmonellas, Campylobacteria, Yersinias, Staphylococci, and Bacillus cereus have been effectively monitored (Hakalehto, Pesola, Heitto, Närvänen, & Heitto, 2007;Hakalehto et al, 2009;Pitkänen et al, 2009;Mentu, Keitel, Heitto, & Hakalehto, 2009). For example, a single Salmonella cell produced a detectable signal (infrared light or ion mobility sensors or traditional medium plate confirmation) in about 10 hours (Hakalehto et al, 2011c).…”

Section: Pmeu In Water Microbiologymentioning

confidence: 99%

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

Hakalehto¹,

Heitto²

2012

ENRR

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Water purity requires continuous monitoring. PMEU technology (Portable Microbe Enrichment Unit) provides the means for starting the enrichment and analysis immediately after the sampling. The entire procedure can be automated and microbe levels monitored in real-time from the enrichment broth by using IR, optical, UV or volatiles screening. For example, single cells of Escherichia coli have been verified from water samples in less than 10 hours in an official validation project. Also such pathogens as Salmonella, Campylobacter and Yersinia have been detected rapidly from natural, purified and irrigation waters. The PMEU technology has potential in the continuous monitoring within water departments and distribution systems, as well as in environmental studies.Keywords: rapid microbe detection, water hygiene, indicator bacteria, ColilertTM, PMEU, environmental monitoring, pathogens, water distribution BackgroundIn the present manuscript experimental approach and work carried out with the Portable Microbe Enrichment Unit (PMEU) is presented. This device is an ideal tool to investigate micro-organisms life at microscopic and molecular scale. Micro-organisms such as bacteria have a large influence on our lives with impact on economy, various industries, healthcare, agriculture and forestry, as well as in water management. In Finland our team published booklet entitled "Vesien Mikrobiaapinen" (in English: "A Microbiological ABC book of the waters") with the purpose to provide microbiological information to decision makers. As scientists we continuously deal with the public and their representatives: the political decision makers. In order to get funding, we ought to make them understand the importance of environmental protection and conservation of water sources, from the microbiological point of view.One area where micro-organisms have a large influence is the threat and deleterious impact on human and animal health caused by pathogenic micro-organisms. They are potentially distributed via waterways and often disseminated at small concentrations which still pose health hazards. In order to detect these minute concentrations, new tools are needed, also to simulate their biochemical activities. Their detection is not either a single or a simple event but a whole diagnostic process (Figure 1). Focusing on the often neglected step of specimen collection is of crucial importance. Sampling should be carried out considering all the physicochemical parameters, selection of sampling site etc. Usually it is required to transport and store the sample with caution and care. This is the step when most of the microbes present in the sample are often destroyed. They simply cannot survive or do not remain microbiologically viable. In order to avoid these problems, the Portable Microbe Enrichment Unit was developed. Time is saved in the PMEU processing of the samples, since the verification steps could follow sooner compared to traditional microbiology. The basic simple idea is to start the enrichment cultivation right subseq...

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Section: Pmeu In Water Microbiologymentioning

confidence: 99%

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

Hakalehto¹,

Heitto²

2012

ENRR

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“…The PMEU method has earlier been validated for the detection of coliformic bacteria [13] and for the enrichment and detection of Campylobacter sp. in waters [11].…”

Section: Introductionmentioning

confidence: 98%

“…1) has been designed to promote recovery and accelerate growth of microbes under aseptic gas flow in adjusted temperatures [6]. It reveals the enteric and other bacteria in clinical, industrial and environmental samples and has also been used in the investigations on the properties of enteric bacteria and interactions between members of the intestinal flora [5,[7][8][9][10][11][12]. The PMEU method has earlier been validated for the detection of coliformic bacteria [13] and for the enrichment and detection of Campylobacter sp.…”

Section: Introductionmentioning

confidence: 99%

Enterobacterial Microflora in Infancy—A Case Study with Enhanced Enrichment

Pesola

Hakalehto

2011

Indian J Pediatr

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The enrichment step turned out to essentially improve the characterization and monitoring of the intestinal enterobacterial microbiota of infants. Compared to plate culture the amount of isolates was 2.6-fold by the PMEU enrichment. This study gives an idea on the development and succession of microbial communities in the gastrointestinal tract and on the variation of the strains due to the intestinal environmental factors.

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“…Further testing on the continuous automated safeguarding of the household waters has been accomplished in Finland [7]. The detection of pathogenic bacteria in waters by the PMEU platform has been carried out for facultatively microaerobic campylobacteria [8], strictly anaerobic Clostridium sp. [9] and anaerobic salmonellas [6].…”

Section: Introductionmentioning

confidence: 99%

“…In tap water artificially contaminated with salmonellas all levels of pathogen load were verified within approximately ten hours using the PMEU Spectrion ® equipped with gas sensors. The PMEU approach turned out to be more efficient in campylobacterial monitoring than the official ISO-method (ISO 17995.2005) in a validation study at the Finnish National Institute of Health and Welfare [8].…”

Section: Introductionmentioning

confidence: 99%

Development of microbiological field methodology for water and food-chain hygiene analysis of Campylobacter spp. and Yersinia spp. in Burkina Faso, West Africa

et al. 2014

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Enhanced enrichment and detection of thermotolerant Campylobacter species from water using the Portable Microbe Enrichment Unit and real-time PCR

Cited by 14 publications

References 26 publications

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

Minute Microbial Levels Detection in Water Samples by Portable Microbe Enrichment Unit Technology

Enterobacterial Microflora in Infancy—A Case Study with Enhanced Enrichment

Development of microbiological field methodology for water and food-chain hygiene analysis of Campylobacter spp. and Yersinia spp. in Burkina Faso, West Africa

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