Enhanced elevations of hypo-osmotic shock-induced cytosolic and nucleic calcium concentrations in tobacco cells by pretreatment with dimethyl sulfoxide

Nguyen, Hieu T H; Bouteau, François; Mazars, Christian; Kuse, Masaki; Kawano, Tomonori

doi:10.1080/09168451.2018.1533801

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…This variable was also enhanced significantly with DMSO when applied for a short time as a unique treatment in Pavon. These differences could reflect the effect of DMSO on different processes due to its ability to induce cell reprogramming through changes in the epigenetic landscape [37,38] or by enhancing the permeability of Ca 2+ [39] that is known to increase ME in barley [40].…”

Section: Option B)mentioning

confidence: 99%

Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route

Castillo

Valero-Rubira

Burrell

et al. 2020

Plants

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Microspores can be developmentally reprogrammed by the application of different stress treatments to initiate an embryogenic pathway leading to the production of doubled haploid (DH) plants. Epigenetic modifications are involved in cell reprogramming and totipotency in response to stress. To increase microspore embryogenesis (ME) efficiency in bread wheat, the effect of the histone deacetylase inhibitor trichostatin A (TSA) has been examined in two cultivars of wheat with different microspore embryogenesis response. Diverse strategies were assayed using 0–0.4 µM TSA as a single induction treatment and after or simultaneously with cold or mannitol stresses. The highest efficiency was achieved when 0.4 µM TSA was applied to anthers for 5 days simultaneously with a 0.7 M mannitol treatment, producing a four times greater number of green DH plants than mannitol. Ultrastructural studies by transmission electron microscopy indicated that mannitol with TSA and mannitol treatments induced similar morphological changes in early stages of microspore reprogramming, although TSA increased the number of microspores with ’star-like’ morphology and symmetric divisions. The effect of TSA on the transcript level of four ME marker genes indicated that the early signaling pathways in ME, involving the TaTDP1 and TAA1b genes, may be mediated by changes in acetylation patterns of histones and/or other proteins.

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Section: Option B)mentioning

confidence: 99%

Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route

Castillo

Valero-Rubira

Burrell

et al. 2020

Plants

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“…In a recent study carried out on hydroponicallycultivated rice seedlings exposed to different DMSO concentrations for 72 h, growth inhibition, protein content alteration, H 2 O 2 accumulation in the root, and changes in antioxidant enzyme activity has been observed (Zhang et al 2016). More recently, it was reported that DMSO enhanced the hypoosmotically induced increases in Ca 2þ concentration in cytosolic and nucleic compartments of transgenic tobacco celllines (BY-2) expressing aequorin (Nguyen et al 2019). These results are in contrast with those presented in this study.…”

Section: Discussionmentioning

confidence: 98%

“…However, it should be considered that Zhang et al (2016) added DMSO to the liquid medium of hydroponic cultures, where it comes into direct contact with the root, whose absorbing power is greater than that of the aerial organs. Also in the study by Nguyen et al (2019), DMSO was added to the liquid medium, therefore, it is not surprising that the specific experimental system used led to a different plant response.…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of dimethyl sulfoxide againstBotrytis cinereaand phytotoxicity on tomato and lettuce plants

Petruccelli

Brasili

Varone

et al. 2020

Plant Biosystems - An International Journal Dealing with all As

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For the first time the antifungal activity of dimethyl sulfoxide (DMSO) was evaluated against Botrytis cinerea, that it is one of the phytopathogenic fungi which causes the greatest damage in agriculture. In in-vitro tests, the greatest inhibitory effect of DMSO on fungal grow was recorded at pH 6. A significant growth inhibition was caused by 0.5% DMSO at 96 h post-inoculation. With higher DMSO concentrations, significant effects were recorded starting from 48 h post-inoculation. As the medium pH decreased, the inhibitory effect of DMSO also decreased. At pH 4 and 5 significant growth inhibition was caused by 1% DMSO starting from 72 h post-inoculation. At all tested pH values, a total growth inhibition was caused by ! 2% DMSO. On tomato leaves infected with B. cinerea, 2% DMSO significantly decreased the extent of damaged leaf area. The administration of DMSO at concentrations ranging from 0.5 to 2% through nebulization on leaves of young Solanum lycopersicum and Lactuca sativa plants did not change the chlorophyll fluorescence (Fv/Fm and U PSII ) at any of the experimental times. Overall, the data obtained suggest that, at the concentrations tested, DMSO is toxic to B. cinerea, while it is well tolerated by lettuce and tomato plants.

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“…In the last 10 years, aequorin has been widely used as a Ca 2+ indicator in a great variety of research: for studying the neural basis for Drosophila behavior [ 44 , 45 , 46 , 47 , 48 , 49 ]; exploring brain activity and visualizing Ca 2+ signaling events that occur at the early stages of zebrafish development [ 44 , 50 , 51 , 52 , 53 , 54 ]; elucidating the Ca 2+ response to biotic or abiotic stress in different plants [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ] and yeast [ 63 ]; as well as for protozoa studies [ 64 ].…”

Section: Analytical Application Of Ca 2+ -Regulmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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: The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

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Enhanced elevations of hypo-osmotic shock-induced cytosolic and nucleic calcium concentrations in tobacco cells by pretreatment with dimethyl sulfoxide

Cited by 5 publications

References 17 publications

Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route

Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route

Antifungal activity of dimethyl sulfoxide againstBotrytis cinereaand phytotoxicity on tomato and lettuce plants

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

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