Enhanced electroscalpel incisional wound healing potential of honey in wistar rats

Abstract

Electrosurgery reduces surgical bleeding, and delayed wound healing. This study evaluated comparative incisional wound healing potential of honey in wound created with electroscalpel and cold scalpel. The study used twelve (12) adult male albino rats (130 ± 20 g), randomly grouped into Electro-cautery (n = 6) and Cold scalpel (n = 6). Each rat had three full thickness (6 mm diameter) skin wounds (a, b and c) created on its dorsum with either Electroscalpel (ES) or Cold blade scalpel (CS), and treated topically with Silver sulphadiazine (SSD, wound a), untreated (control, wound b) and Bee honey (H, wound c). The wounds were evaluated for gross (exudation, edema, hyperemia, contraction), histologic (granulation, angiogenesis, fibroplasia, epithelialization) and immunologic healing indices using standard techniques. Data were analyzed with Chi-square, Two-way Analysis of Variance (ANOVA) and Duncan Multiple Range tests (DMRT) at α = 0.05. Wound hyperemia and edge edema were prominent in the ES group from day 4 to 6 (P = .000). Percentage wound contraction was higher in the CS than ES group from days 5 to 7 (P = .006) and in the CS treated with honey than ES treated with honey from days 7 to 14 (P = .000). Granulation tissue reduced in ES group treated with SSD than in honey and control wounds. Fibroelastic tissue increased in SSD and honey treated wounds of ES group, and higher in honey treated wounds of CS group (P < .05). Fibroplasia was sustained in honey and SSD treated wounds than control. Honey can be applied to electroscalpel surgical wound to facilitate rapid healing during surgical management of tumours having vascular network.

Mechanisms of ivermectin-induced wound healing

“…This was done with the aid of Imito Measure® Application (Imito AG, Flüelastrasse, Zürich, Switzerland) and the Java-based Image Processing and Analysis software, ImageJ Software (National Institutes of Health, USA). The time-course of cutaneous wound healing was determined as the wound contraction or closure rate using the formula [ 36 ]: where WC = Wound Contraction, WA = Wound Area, d 0 = Day 0 and d n = Day n or any other day.…”

“…The wounds were evaluated in vivo on day 0, 2, 5, 7, 9, day 13, 15, and on day 21 for cutaneous wound healing indices such as Exudation, Edge edema; Hyperemia, and Granulation Tissue deposition based on methods described by Gupta et al (2011) [35] and subsequently modified by Eyarefe et al(2017 ) [36]. Wound Exudation, Wound Edge Edema; Wound Hyperemia was scored by two blinded pathologists working independently as 0 = Absent, 1-2 = Mild, 3-4 = Moderate, 5-6 = Severe.…”

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

“…Moreover, a recent study had shown enhanced healing of electroscalpel-induced wound of Wistar rats by EBH treatment, as compared to silver sulfadiazine which is being used as positive control [68]. Another in vivo study also proved that oral administration of EBH to measure the healing of colonic anastomosis in rats, had shown increment of the tensile strength measured by bursting pressure, increased fibroblast count and lowered mean of inflammatory cells count in rats supplemented with honey after the surgical procedure in comparison with the control group [70, 102].…”

Untitled

“…Essa propriedade é amplamente fundamentada na literatura científica e considerada uma das propriedades medicinais mais antigas descobertas na utilização dessa matéria-prima no âmbito medicinal. Tal propriedade está relacionada com as atividades imunoestimuladoras do mel no crescimento celular epitelial e do tecido conjuntivo, além de também atuar no desenvolvimento de novos capilares 35 . Outra linha terapêutica que vem sendo indicada como potencial para utilização do mel é a do tratamento adjuvante para tumores cerebrais uma vez que a utilização desse produto apresentou resultados promissores em um efeito antiproliferativo e antimetastático das células tumorais 31 .…”