Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection

Park, Seonhwa; Jeong, Ji Eun; Le, Van Sang; Seo, Jong-Soo; Yu, Byeongjun; Kim, Da Young; Kwon, Sun-Hong; Jon, Sangyong; Woo, Han Young; Yang, Haesik

doi:10.1021/jacs.7b12382

Cited by 23 publications

(15 citation statements)

References 18 publications

(32 reference statements)

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“…Long‐range electron transfer (≈ 18 nm for 10 kDa polyelectrolytes) has recently been demonstrated with anionic π‐conjugated polyelectrolyte (CPE) with multiple redox labels along the CPE. [ 24 ] However, due to the cross‐linked nature of plasma polymer films, this effect cannot be attributed to π‐conjugation. In fact, the differences in charge transfer behavior observed through MePOx and PiPOx films seem to indicate that the mechanism is closely linked to ion permeability.…”

Section: Resultsmentioning

confidence: 99%

Plasma Deposited Polyoxazoline Thin Films for the Biofunctionalization of Electrochemical Sensors

Eulate

Gheorghiu

Amoura

et al. 2021

Adv Materials Technologies

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Electrochemical immunosensors are an emerging technology for the fast, sensitive, and reliable diagnosis of diseases from bodily fluids. These sensors work by detecting a change in current upon analyte binding to an immuno‐functionalized electrode. Current methods of electrode functionalization are lengthy processes involving self‐assembled monolayer formation and wet chemistry biofunctionalization. Herein, thin films deposited from the plasma phase of oxazoline precursors are investigated and optimized as an alternative approach for electrode functionalization. The plasma‐enabled method has the advantage of being substrate independent and allows the spontaneous binding of biomolecules in physiological buffer. Surface sensitive analysis techniques are employed to characterize the thickness, reactivity, and stability of the thin films before investigating their electrochemical properties on indium tin oxide and gold electrodes including the feasibility to reduce charge transfer resistance with gold nanoparticles. Last, these films are employed to develop an immunosensor for the detection of free epithelial cell adhesion molecule with a limit of detection of 8.7 ng mL−1.

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Section: Resultsmentioning

confidence: 99%

Plasma Deposited Polyoxazoline Thin Films for the Biofunctionalization of Electrochemical Sensors

Eulate

Gheorghiu

Amoura

et al. 2021

Adv Materials Technologies

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“…Distance‐dependent signals have played crucial roles in washing‐free homogeneous and heterogeneous bioassays . When a target (bio)molecule is bound to a biosensing probe or surface, the distance change between a signaling species (or a label that generates a signaling species) and a signal‐enhancing or ‐interfering species (or a signal‐measuring surface) induces significant signal variation.…”

Section: Introductionmentioning

confidence: 99%

“…The optical near field (i.e., evanescent field) generated under the condition of total internal reflection or surface plasmon resonance can only excite fluorescent labels near a solid surface and cannot excite the labels far from the surface. In washing‐free electrochemical bioassays, distance‐dependent electron transfer, such as i) distance‐dependent direct electron transfer between a signaling label and an electrode and ii) distance‐dependent mediated electron transfer of an electron mediator between a catalytic label and an electrode, has been used . However, the combined use of distance‐dependent electromagnetic radiation and electron transfer has not been reported in washing‐free bioassays.…”

Section: Introductionmentioning

confidence: 99%

Surface‐Plasmonic‐Field‐Induced Photoredox Catalysis and Mediated Electron Transfer for Washing‐Free DNA Detection

Kim

Park

et al. 2020

Angew Chem Int Ed

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Distance-dependent electromagnetic radiation and electron transfer have been commonly employed in washingfree fluorescence and electrochemical bioassays,r espectively. In this study,w ec ombined the two distance-dependent phenomena for sensitive washing-free DNAd etection. A distance-dependent surface plasmonic field induces rapid photoredox catalysis of surface-bound catalytic labels,a nd distance-dependent mediated electron transfer allows for rapid electron transfer from the surface-bound labels to the electrode. An optimal system consists of achemically reversible acceptor (Ru(NH 3) 6 3+), ac hemically reversible photoredoxc atalyst (eosin Y), and ac hemically irreversible donor (triethanolamine). Side reactions with O 2 do not significantly decrease the efficiency of photoredox catalysis.E nergy transfer quenching between the electrode and the label can be lowered by increasing the distance between them. Washing-free DNA detection had ad etection limit of approximately 0.3 nm in buffer and 0.4 nm in serum without aw ashing step.

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“…On the other hand, anionic CPEs have been widely applied in the biological and chemical analysis area with their excellent water solubility. Direct electron transfer over a longer working distance can be accomplished through the sulfonated conjugate polyelectrolyte, which has a strong signal in DNA detection (12). And a probe system as fluorescence turn-on assay of simple saccharides is based on three anionic conjugated polyelectrolytes and three quenched molecules substituted by boric acid (13).…”

Section: Introductionmentioning

confidence: 99%

Novel benzimidazole-based conjugated polyelectrolytes: synthesis, solution photophysics and fluorescent sensing of metal ions

Wei

et al. 2020

E-Polymers

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AbstractTwo benzimidazole-based conjugated polyelectrolytes (+)-PPBIPV and (-)-PPBIPV which have opposite charges on their side chains were synthesized via Heck coupling reaction and characterized by 1H-NMR, UV-vis and PL spectroscopy. These two polyelectrolytes are both consisted of benzimidazole derivatives and phenylenevinylene units. The absorption and emission spectra reveal that the polymers both have solvent-dependency and concentration-dependency, and they exhibit aggregation effect in aqueous solution. In the respect of ion detection, the aqueous solution of (+)-PPBIPV has excellent selectivity and sensitivity for Fe3+. Moreover, Pd2+ can almost completely quench the fluorescence of (+)-PPBIPV in methanol solution, and its quenching constant KSV is 5.93×104 M-1. For (-)-PPBIPV, Sn2+ can double the fluorescence intensity of its aqueous solution, while (-)-PPBIPV has good identification for Fe3+ in methanol with a KSV = 3.44×105 M-1. Hence, two polyelectrolytes have considerable potential to become effective fluorescent sensing materials for some specific metal ions. All of the stoichiometric relationships between metal ions and conjugated polyelectrolytes were calculated using Benesi-Hildebrand equation.

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Enhanced Electron Transfer Mediated by Conjugated Polyelectrolyte and Its Application to Washing-Free DNA Detection

Cited by 23 publications

References 18 publications

Plasma Deposited Polyoxazoline Thin Films for the Biofunctionalization of Electrochemical Sensors

Plasma Deposited Polyoxazoline Thin Films for the Biofunctionalization of Electrochemical Sensors

Surface‐Plasmonic‐Field‐Induced Photoredox Catalysis and Mediated Electron Transfer for Washing‐Free DNA Detection

Novel benzimidazole-based conjugated polyelectrolytes: synthesis, solution photophysics and fluorescent sensing of metal ions

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