Enhanced Efficiency of Rna-guided Cas12a Versus Cas9 Transgene Knock-in and Activity at a Schistosoma Mansoni Genome Safe Harbor
Wannaporn Ittiprasert,
Max Moescheid,
Prapakorn Wisitphongpun
et al.
Abstract:Recently, we reported programmed Cas9 mediated insertion of a reporter gene into a gene safe harbor site, GSH1, of Schistosoma mansoni via homology-directed repair (HDR) using overlapping guide RNAs. Here, we report efficient and precise CRISPR/Cas12a-mediated homology directed insertion (knockin, KI) of a 5’ C6-PEG10-modified double-stranded transgene bearing microhomology arms, 50 nt in length, at GSH1. At the outset, we undertook bioinformatic and computational analysis following by experimental verificatio… Show more
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