Enhanced direct fermentation from food waste to butanol and hydrogen by an amylolytic Clostridium

Zhang, Chen; Li, Tinggang; Su, Guandong; He, Jianzhong

doi:10.1016/j.renene.2020.01.151

Cited by 56 publications

(15 citation statements)

References 34 publications

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“…Zhang et al [297] recently used an amylolytic Clostridium species to produce butanol and hydrogen from food waste simultaneously. It was also shown that the supplementation of calcium ions increased the butanol yield by more than 17.7% because these additives enhanced the amylase activity [298]. Similarly, Cao et al [311] observed that the co-valorization of corn steep liquor (CSL) and paper mill sludge (PMS) resulted in higher butanol production.…”

Section: Butanolmentioning

confidence: 96%

“…Butanol is an ethyl alcohol that is mainly used as a chemical intermediate, solvent, and extractant in various commercial applications such as cosmetics and pharmaceutical industries and also used in the production of other chemicals such as butyl acrylate and methacrylate [297,298]. It consists of four isomeric structures, namely the n-butanol (n-C 4 H 9 OH), sec-butanol (sec-C 4 H 9 OH), iso-butanol (iso-C 4 H 9 OH), and tert-butanol (tert-C 4 H 9 OH) [299,300].…”

Section: Butanolmentioning

confidence: 99%

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Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Sekoai

Ghimire

Ezeokoli

et al. 2021

Renewable and Sustainable Energy Reviews

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In recent years, much attention has been directed towards the integration of dark fermentation process into a biorefinery concept to enhance the energetic gains, thereby improving the competitiveness of this process. The volatile fatty acids (VFAs) from dark fermentative H 2 -producing processes serve as precursors for the microbial synthesis of a broad spectrum of biotechnologically-important products such as biofuels and biocommodities. These products are desirable substrates for secondary bioprocesses due to their biodegradable nature and affordability. This short review discusses the use of acidogenic-derived VFAs in the production of value-added compounds such as polyhydroxyalkanoates (PHAs) alongside the microbial-based fuels (hydrogen, biogas, and electricity), and other valuable compounds (succinic acid, citric acid, and butanol). The review also highlights the strategies that have been used to enhance the extraction of VFAs from acidogenic effluents and other related waste streams. The application of novel enhancement techniques such as nanoparticles during VFAs recovery is also discussed in this work. Furthermore, the work highlights some of the recent advances in dark fermentationbased biorefinery, particularly the development of pilot-scale processes. Finally, the review provides some suggestions on the advancement of dark fermentation-based biorefineries using VFAs that are derived from acidogenic processes.

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Section: Butanolmentioning

confidence: 96%

Section: Butanolmentioning

confidence: 99%

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Sekoai

Ghimire

Ezeokoli

et al. 2021

Renewable and Sustainable Energy Reviews

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“…In addition, with the development of n-butanol production technology, the production cost of n-butanol has gradually reduced. N-butanol is prepared from a wide range of raw materials with low prices, mainly wheat, corn, pulp waste liquid, molasses and wild plants [ 9 , 10 , 11 , 12 ]. These characteristics make n-butanol more advantageous than methanol and ethanol as an alternative fuel for internal combustion engines and it has become one of the most promising alternative fuels for internal combustion engines.…”

Section: Introductionmentioning

confidence: 99%

An Experimental Study on Combustion and Cycle-by-Cycle Variations of an N-Butanol Engine with Hydrogen Direct Injection under Lean Burn Conditions

Shang

Shi

et al. 2022

Sensors

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This study experimentally investigated the effects of hydrogen direct injection on combustion and the cycle-by-cycle variations in a spark ignition n-butanol engine under lean burn conditions. For this purpose, a spark ignition engine installed with a hydrogen and n-butanol dual fuel injection system was specially developed. Experiments were conducted at four excess air ratios, four hydrogen fractions(φ(𝐻2)) and pure n-butanol. Engine speed and intake manifold absolute pressure (MAP) were kept at 1500 r/min and 43 kPa, respectively. The results indicate that the θ0–10 and θ10–90 decreased gradually with the increase in hydrogen fraction. Additionally, the indicated mean effective pressure (IMEP), the peak cylinder pressure (Pmax) and the maximum rate of pressure rise ((dP/dφ)max) increased gradually, while their cycle-by-cycle variations decreased with the increase in hydrogen fraction. In addition, the correlation between the (dP/dφ)max and its corresponding crank angle became weak with the increase in the excess air coefficient (λ), which tends to be strongly correlated with the increase in hydrogen fraction. The coefficient of variation of the Pmax and the IMEP increased with the increase in λ, while they decreased obviously after blending in the hydrogen under lean burn conditions. Furthermore, when λ was 1.0, a 5% hydrogen fraction improved the cycle-by-cycle variations most significantly. While a larger hydrogen fraction is needed to achieve the excellent combustion characteristics under lean burn conditions, hydrogen direct injection can promote combustion process and is beneficial for enhancing stable combustion and reducing the cycle-by-cycle variations.

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“…However, the consortia fermentation would be more difficult to operate and scale up since the cellulolytic and solventogenic clostridia usually require different nutrients, pH, and/or temperature for optimal growth. On the other hand, numerous studies have also been directed toward isolating or engineering cellulolytic clostridia that can directly convert lignocellulosic or other renewable biomass into butanol (Gaida et al, 2016;Higashide et al, 2012;Li et al, 2018;Lin et al, 2015;Tian et al, 2019;Xin et al, 2018;Yang et al, 2015;Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Engineering Clostridium cellulovorans for highly selective n‐butanol production from cellulose in consolidated bioprocessing

Teng

Hou

et al. 2021

Biotech & Bioengineering

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Cellulosic n-butanol from renewable lignocellulosic biomass has gained increased interest. Previously, we have engineered Clostridium cellulovorans, a cellulolytic acidogen, to overexpress the bifunctional butyraldehyde/butanol dehydrogenase gene adhE2 from C. acetobutylicum for n-butanol production from crystalline cellulose. However, butanol production by this engineered strain had a relatively low yield of approximately 0.22 g/g cellulose due to the coproduction of ethanol and acids. We hypothesized that strengthening the carbon flux through the central butyryl-CoA biosynthesis pathway and increasing intracellular NADH availability in C. cellulovorans adhE2 would enhance n-butanol production. In this study, thiolase (thlA CA ) from C. acetobutylicum and 3-hydroxybutyryl-CoA dehydrogenase (hbd CT ) from C. tyrobutyricum were overexpressed in C. cellulovorans adhE2 to increase the flux from acetyl-CoA to butyryl-CoA. In addition, ferredoxin-NAD(P) + oxidoreductase (fnr), which can regenerate the intracellular NAD(P)H and thus increase butanol biosynthesis, was also overexpressed. Metabolic flux analyses showed that mutants overexpressing these genes had a significantly increased carbon flux toward butyryl-CoA, which resulted in increased production of butyrate and butanol.The addition of methyl viologen as an electron carrier in batch fermentation further directed more carbon flux towards n-butanol biosynthesis due to increased reducing equivalent or NADH. The engineered strain C. cellulovorans adhE2-fnr CA -thlA CAhbd CT produced n-butanol from cellulose at a 50% higher yield (0.34 g/g), the highest ever obtained in batch fermentation by any known bacterial strain. The engineered C. cellulovorans is thus a promising host for n-butanol production from cellulosic biomass in consolidated bioprocessing.

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Enhanced direct fermentation from food waste to butanol and hydrogen by an amylolytic Clostridium

Cited by 56 publications

References 34 publications

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

An Experimental Study on Combustion and Cycle-by-Cycle Variations of an N-Butanol Engine with Hydrogen Direct Injection under Lean Burn Conditions

Engineering Clostridium cellulovorans for highly selective n‐butanol production from cellulose in consolidated bioprocessing

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