Enhanced development of somatic embryos of Plantago ovata Forsk. By additives

Das, M. R.; Raychaudhuri, Sarmistha Sen

doi:10.1007/s11627-001-0099-6

Cited by 22 publications

(19 citation statements)

References 8 publications

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“…A possible explanation for this result is that the high concentration of 2,4-D, singularly, in the medium may inhibit callus growth from hypocotyl explants. Das and Raychaudhury [19] reported that combination of 4.5 μm 2,4-D and 2.3 μM Kin were more effective for callus initiation from shoot buds of P. ovata. The highest callus diameter (2.83 mm) in P. ovata was reported in B 5 tissue culture media [28], but according to these findings, the MS medium with combination of 2,4-D and Kin was strongly affected on increasing callus diameter (4.25 mm) in P. ovata.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is defined as the use of high doses of 2,4-D, solely dose not leads to increase in cell division and cell elongation, which is consistent with our results showing the requirement of both 2,4-D with Kin for rapid callus growth rate. Literature review showed that callus growth has been studied in a base of callus weight changes in P. ovata [19,21]. This novel equation could discriminate the reaction of callus growth rate (mm/day) at different times after callus induction in P. ovata.…”

Section: Discussionmentioning

confidence: 99%

“…With the above mentioned difficulties, callus culture has been an alternative and efficient source for the production of higher secondary metabolites using of somaclonal variation [18]. Different explants including shoot buds [19], hypocotyl [20], leaf [7] and root [21] have been used to induce callus in P. ovata. The optimization of medium ingredients, especially plant growth hormones, was the basic approaches in bioreactor system of the plant for mucilage production.…”

Section: Introductionmentioning

confidence: 99%

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Experimental Paper. In vitro synthesis of mucilage in Plantago ovata Forsk affected by genotypes and culture media

2017

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Summary Introduction: Psyllium (Plantago ovata Forsk) is medicinally used mainly for its mucilage content. Objective: In the present study, an attempt was made to improve mucilage yield under in vitro callus culture using different genotypes, explants and culture media. Methods: The effects of a range of concentrations of plant growth regulators including 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (Kin) were evaluated on mucilage synthesis under in vitro culture using cotyledon, hypocotyl and seed explants. Fourteen genotypes originating from different geographical regions of Iran were used to evaluate their response to in vitro mucilage synthesis. Results: The highest rate of callus induction (76%) and callus growth rate CGR (0.38 mm/day) were induced on MS medium supplemented with 0.5 mg/l 2,4-D and 1 mg/l Kin and the hypocotyl explant. The results of analysis of variance showed significant genotypic differences for callus induction, CGR and mucilage content of callus and seeds. The mucilage content ranged from 0.38 to 0.08 (g/g DW) and 0.13 to 0.042 (g/g DW) for callus and seed, respectively. The superior callus induction (73%), CGR (0.45 mm/day) and mucilage content of callus (0.38 g/g DW) was denoted to Po1 genotype. The callus produced nearly three times more mucilage than the seeds using superior genotype (Po1). Conclusion: The results of this study revealed that high efficiency of callus culture of P. ovata using hypocotyl explant accompanied by the exploration of genetic diversity are important to improve the yield of mucilage synthesis by in vitro callus culture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Experimental Paper. In vitro synthesis of mucilage in Plantago ovata Forsk affected by genotypes and culture media

2017

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“…To enhance the efficiency of embryo production, additives like casein hydrolysate and filtered green coconut water have been used earlier by our group in Plantago ovata [14].…”

Section: Introductionmentioning

confidence: 99%

“…In the present investigation, P. ovata was chosen as the test system because it is amenable to tissue culture, and the protocol for plant regeneration from somatic embryo has been standardised in our laboratory [14]. The plant has tremendous medicinal importance and is exported from India to various countries of the world.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Trace Elements During Different Developmental Stages of Somatic Embryogenesis in Plantago ovata Forssk Using Energy Dispersive X-ray Fluorescence

Saha

Raychaudhuri

Sudarshan

et al. 2009

Biol Trace Elem Res

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Energy dispersive X-ray fluorescence (ED-XRF) technique has been used for the determination of trace element profile during different developmental stages of somatic embryogenic callus of an economically important medicinal plant, Plantago ovata Forssk. Somatic embryogenesis is a plant tissue culture-based technique, which is used for plant regeneration and crop improvement. In the present investigation, elemental content was analysed using ED-XRF technique during different developmental stages and also determine the effect of additives--casein hydrolysate and coconut water on the trace elemental profile of embryogenic callus tissue of P. ovata. Subsequent experiments showed significant alteration in the concentration of K, Ca, Mn, Fe, Zn, Cu, Br, and Sr in both the embryogenic and non-embryogenic callus. Higher K, Ca, Fe, Cu, and Zn accumulation was in embryogenic tissue stage compared to other stages, suggesting these elements are crucial for successful embryogenesis. The results suggest that this information could be useful for formulating a media for in vitro embryo induction of P. ovata.

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Antioxidant activity and high‐performance liquid chromatographic analysis of phenolic compounds during in vitro callus culture of Plantago ovata Forsk. and effect of exogenous additives on accumulation of phenolic compounds

et al. 2015

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Enhanced development of somatic embryos of Plantago ovata Forsk. By additives

Cited by 22 publications

References 8 publications

Experimental Paper. In vitro synthesis of mucilage in Plantago ovata Forsk affected by genotypes and culture media

Experimental Paper. In vitro synthesis of mucilage in Plantago ovata Forsk affected by genotypes and culture media

Analysis of Trace Elements During Different Developmental Stages of Somatic Embryogenesis in Plantago ovata Forssk Using Energy Dispersive X-ray Fluorescence

Antioxidant activity and high‐performance liquid chromatographic analysis of phenolic compounds during in vitro callus culture of Plantago ovata Forsk. and effect of exogenous additives on accumulation of phenolic compounds

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