Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells

Jin, Hai-feng; Kumar, B. Mohana; Kim, Jung-Gon; Song, Hye Young; Jeong, Yeon-Ji; Cho, Seong-Keun; Balasubramanian, S.; Choe, Sang-Yong; Rho, Gyu‐Jin

doi:10.1387/ijdb.062165hj

Cited by 60 publications

(66 citation statements)

References 23 publications

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“…However, no statistically significant difference in fusion rate, cleavage rate, and blastocyst rate was found between in vitro-cultured MSCs-embryos and Fsembryos of three investigated swine breeds. This is not consistent with that seen in previous studies (Colleoni et al, 2005;Faast et al, 2006;Jin et al, 2007;Kumar et al, 2007;Lee et al, 2010), which all reported that the MSCs-embryos have a significantly ( p £ 0.05) higher blastocyst rate than Fs-embryos.…”

contrasting

confidence: 98%

“…It was reported that in cultured porcine embryos cloned from MSCs (MSCs-embryos), the expression pattern of developmentally important genes involved in pluripotency, epigenetic modification, imprinting, and apoptosis was more similar with that of in vivo-derived embryos, as compared with embryos cloned from Fs (Fs-embryos) . Moreover, several studies have reported that the in vitro preimplantation developmental potential of porcine MSCs-embryos was significantly higher than that of Fsembryos (Colleoni et al, 2005;Faast et al, 2006;Jin et al, 2007;Kumar et al, 2007;Lee et al, 2010).…”

mentioning

confidence: 99%

“…However, because there are no porcine ESCs with proven pluripotency available now, MSCs, a type of multipotent adult stem cells, were employed as donor cells to improve the success rate of pig cloning. Although the porcine MSCs-embryos were demonstrated to have higher preimplantation developmental competence than Fs-embryos in in vitro culture (Colleoni et al, 2005;Faast et al, 2006;Jin et al, 2007;Kumar et al, 2007;Lee et al, 2010), their full-term developmental ability has not yet been compared with that of Fsembryos. In this study, we showed that porcine MSCs-embryos have higher blastocyst total cell numbers as well as full-term developmental potential, and porcine MSCs can be reprogrammed to generate live transgenic cloned pigs following stable genetic modification.…”

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Bone Marrow Mesenchymal Stem Cells Are an Attractive Donor Cell Type for Production of Cloned Pigs As Well As Genetically Modified Cloned Pigs by Somatic Cell Nuclear Transfer

He²,

Chen³

et al. 2013

Cellular Reprogramming

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The somatic cell nuclear transfer (SCNT) technique has been widely applied to clone pigs or to produce genetically modified pigs. Currently, this technique relies mainly on using terminally differentiated fibroblasts as donor cells. To improve cloning efficiency, only partially differentiated multipotent mesenchymal stem cells (MSCs), thought to be more easily reprogrammed to a pluripotent state, have been used as nuclear donors in pig SCNT. Although in vitro-cultured embryos cloned from porcine MSCs (MSCs-embryos) were shown to have higher preimplantation developmental ability than cloned embryos reconstructed from fibroblasts (Fs-embryos), the difference in in vivo full-term developmental rate between porcine MSCs-embryos and Fs-embryos has not been investigated so far. In this study, we demonstrated that blastocyst total cell number and full-term survival abilities of MSCs-embryos were significantly higher than those of Fs-embryos cloned from the same donor pig. The enhanced developmental potential of MSCs-embryos may be associated with their nuclear donors' DNA methylation profile, because we found that the methylation level of imprinting genes and repeat sequences differed between MSCs and fibroblasts. In addition, we showed that use of transgenic porcine MSCs generated from transgene plasmid transfection as donor cells for SCNT can produce live transgenic cloned pigs. These results strongly suggest that porcine bone marrow MSCs are a desirable donor cell type for production of cloned pigs and genetically modified cloned pigs via SCNT.

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confidence: 98%

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confidence: 99%

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Bone Marrow Mesenchymal Stem Cells Are an Attractive Donor Cell Type for Production of Cloned Pigs As Well As Genetically Modified Cloned Pigs by Somatic Cell Nuclear Transfer

He²,

Chen³

et al. 2013

Cellular Reprogramming

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“…These data are substantially in accordance with our data showing that 14.5% of embryos cloned with FFs developed to blastocysts. MSCs have been successfully established, and these cells have been further employed as nuclear donors for porcine NT [6][7][8]33]. Similarly, pig embryos cloned with undifferentiated MSCs and their derivatives along the osteogenic lineage gave rise to consistently high development rates that are comparable to those of adult fibroblasts 1 One-year-old female (crossbred of Landrace × Yorkshire).…”

Section: Discussionmentioning

confidence: 99%

Developmental Ability of Miniature Pig Embryos Cloned with Mesenchymal Stem Cells

Lee

Kang

Maeng

et al. 2010

Journal of Reproduction and Development

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Abstract. The present study compared the developmental ability of miniature pig embryos cloned with fetal fibroblasts (FFs), bone marrow-derived mesenchymal stem cells (MSCs) and differentiated (osteocytes, adipocytes and chondrocytes) MSCs. MSCs were isolated from an approximately 1-month-old female miniature pig (T-type, PWG Micro-pig ® , PWG Genetics Korea). MSCs were differentiated into osteocytes, adipocytes and chondrocytes under controlled conditions and characterized by cell surface antigen profile using specific markers. These differentiated or undifferentiated MSCs, as well as FFs of miniature pig, were transferred into enucleated oocytes of domestic pigs. Data from 10 replicates involving 1567 cloned embryos were assessed in terms of developmental rates. The in vitro development rate to the blastocyst stage of embryos cloned with undifferentiated MSCs was significantly (P<0.05) higher than that of embryos cloned with differentiated MSCs and FFs. Surgical transfer of 523 two-cell stage embryos cloned with undifferentiated MSCs into five synchronized domestic pig recipients resulted in 5 cloned miniature pig offspring (1 stillborn and 4 viable) from 2 pregnant recipients. The results imply that MSCs might be multipotent and that they can be used to produce viable cloned miniature pigs that cannot be easily reproduced with differentiated somatic cells. Key words: Animal cloning, Differentiation, Mesenchymal stem cells, Miniature pig, Nuclear transfer (J. Reprod. Dev. 56: [256][257][258][259][260][261][262] 2010) he production of cloned animals by nuclear transfer (NT) has been increasingly studied as a potential approach to tissue and organ xenotransplantation with reduced immunogenicity for endstage organ failure. Several studies have suggested that miniature pigs, rather than nonhuman-primate species, are suitable as a donor animal for human xenotransplantation [1,2] because miniature pigs are superior in terms of similarities in gross anatomy and physiology to humans as well as in terms of ethical issues. The NT efficiency, however, remains relatively low due to abnormalities throughout pre-and post-implantation development regardless of the species or type of donor cell [3]. A number of factors affect NT efficiency, including the NT technical process, activation protocol, recipient oocyte age, cell cycle stage and type of donor cells, which has been implicated in the precise reprogramming of chromatin and genomic imprinting. Among these factors, incomplete selection of donor cells has also been considered a prime cause for NT inefficiency.Transfer of embryonic stem (ES) cells to produce cloned embryos has resulted in consistently higher numbers of viable offspring compared with somatic cells in mice [4,5]. Moreover, embryos cloned with porcine mesenchymal stem cells (MSCs) and their derivatives along the osteogenic lineage give rise to an increase in the rate of preimplantation development compared with adult fibroblasts [6]. Our previous study on gene expression profiles demonstrated that some ge...

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“…A proliferative ability and higher plasticity are the valuable traits of stem cells compared to differentiated somatic cells (62). Experiments on the mice neural stem cells showed an easier reprogramming of stem or progenitor cells comparing to differentiated cells, moreover, when stem cell nuclei were used as karyoplast the number of cloned embryos significantly increased (63). Nowadays, mesenchymal stem cells are considered the most attractive source of nuclei for cloning (64)(65)(66).…”

Section: First Cloned Progeny In Different Animal Speciesmentioning

confidence: 99%

CRYOBANKING OF SOMATIC CELLS IN CONSERVATION OF ANIMAL GENETIC RESOURCES: PROSPECTS AND SUCCESSES (review)

Сингина¹,

Волкова²,

Багиров³

et al. 2014

S-h. biol.

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A b s t r a c tExtinction of many species is irreversible and is a part of the natural evolution, but human activities have influenced this process, making it much faster comparing to speciation. According to FAO, approximately 20 % of the breeds of cattle, goats, pigs, horses and poultry in the world are currently at risk of disappearance, many have died in the past few years, as a result their genetic characteristics lost forever. The role of banks in the management of genetic resources and the conservation of endangered species is particularly noticeable in the last decade. Most cryobanks focus on the cryopreservation of gametes (primarily sperm) and embryos. Their main goal is to produce offspring using reproductive technologies, which include artificial insemination, in vitro fertilization and embryo transfer. The discovery of the phenomenon of reprogramming somatic cell nuclear allowed expanding the range of forms of biological material in programs for cryopreservation. Creating cryobanks of somatic cells as donors of nuclei for cloning considered an auxiliary instrument for the preservation and improvement of the gene pool of farm animals and poultry. To obtain viable cryopreserved cell lines very small amount of biopsy material, including that of dead animals, is sufficient, but such lines contain the complete genome and proteome. In contrast to germ cells, embryos and generative tissues, the cryopreserved somatic cells after repeated thawing are capable to regenerate, i.e. almost infinitely may serve as a source of biomaterial for use in assisted reproductive technologies and biological research, including retrospective reconstruction. Furthermore, due to the small size the somatic cells are more resistant to cryopreservation. This review also provides a brief description of the principles and history of cloning. The advantages of the use of different cell types as karyoplasts are discussed. In particular, almost all types of cells (e.g. embryonic cells, mammary cells, cumulus, granulosa, oviduct, liver, fibroblasts, white blood cells and embryonic stem cells) can be used for the production of cloned animals, but the cloning efficiency depends significantly on the type of cells. Aiming embryo development and birth of live offspring, the fetal fibroblasts as donors of nuclear material for cloning are most effective. Alternatively, the stem cells may be a source of the nuclei. Stem or progenitor cells (i.e., stem, determined to differentiate in specific type cells) are easier reprogrammed than terminally differentiated cells. Also when stem cells nuclei are used as karyoplasts the number of cloned embryos significantly increased. The advances in interspecific cloning as a strategy for restoration of rare and endangered species are discussed. Numerous examples show that somatic cells can be considered the most promising material for the recovery of animal genetic resources of different types. Particularly from 1997 to 2012 using the differentiated somatic cells domestic and wild animals of different specie...

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Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells

Cited by 60 publications

References 23 publications

Bone Marrow Mesenchymal Stem Cells Are an Attractive Donor Cell Type for Production of Cloned Pigs As Well As Genetically Modified Cloned Pigs by Somatic Cell Nuclear Transfer

Bone Marrow Mesenchymal Stem Cells Are an Attractive Donor Cell Type for Production of Cloned Pigs As Well As Genetically Modified Cloned Pigs by Somatic Cell Nuclear Transfer

Developmental Ability of Miniature Pig Embryos Cloned with Mesenchymal Stem Cells

CRYOBANKING OF SOMATIC CELLS IN CONSERVATION OF ANIMAL GENETIC RESOURCES: PROSPECTS AND SUCCESSES (review)

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