Enhanced detoxification of exogenous toluene gas in transgenic Ardisia pusilla expressing AtNDPK2 gene

Ahn, Chang Ho; Kim, Nan-Sun; Shin, Ju Young; Lee, Young Ah; Kim, Jeong-Ho; Park, Pil Man; An, Hye Ryun; Kim, Yae Jin; Kim, Wonhee; Lee, Su Young

doi:10.1007/s13580-020-00275-1

Cited by 6 publications

(7 citation statements)

References 48 publications

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“…The expression of NDPK1 in the toluene-treated group was 23.2-fold higher than that in the untreated group (Additional file 1: Table S4 ). A previous study also reported that NDPK2 transgenic A. pusilla plants show significantly increased toluene uptake compared to non-transgenic plants [ 14 ].…”

Section: Resultsmentioning

confidence: 93%

“…Our previous study showed that the detoxification ability of AtNDPK2 -transgenic A. pusilla plants is enhanced by the exogenous application of toluene [ 14 ]. In the current study, the results of DEG analysis showed that AtNDPK1 expression was upregulated in toluene-treated A. pusilla .…”

Section: Resultsmentioning

confidence: 99%

“…We speculate that NDPK1 plays an important role in toluene detoxification and metabolism. Previous studies have shown that the overexpression of NDPK2 enhances tolerance to various abiotic stresses [ 17 , 18 ], especially in favor of the detoxification ability of A. pusilla to toluene [ 14 ]. To verify whether the overexpression of AtNDPK2 affects the expression of other genes related to toluene reaction or metabolism, we used qRT-PCR to detect the expression of related DEGs after toluene treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Ardisia pusilla ( A. pusilla ) is a shrub with light green leaves, white flowers, and red berries. It is a popular indoor ornamental plant in Korea [ 14 ]. According to Song, the removal efficiency of toluene by A. pusilla is significantly higher than that of other plants [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…According to Song, the removal efficiency of toluene by A. pusilla is significantly higher than that of other plants [ 15 ]. Ahn et al overexpressed the AtNDPK2 gene in A. pusilla to further improve its ability to absorb toluene [ 14 ]. Previous studies on the AtNDPK2 gene have mainly reported that it can improve abiotic stress tolerance in plants [ 11 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Analysis for the Identification of Metabolic Pathway Genes Related to Toluene Response in Ardisia pusilla

Ahn

Shin

et al. 2021

Plants

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Toluene is an industrial raw material and solvent that can be found abundantly in our daily life products. The amount of toluene vapor is one of the most important measurements for evaluating air quality. The evaluation of toluene scavenging ability of different plants has been reported, but the mechanism of plant response to toluene is only partially understood. In this study, we performed RNA sequencing (RNA-seq) analysis to detect differential gene expression in toluene-treated and untreated leaves of Ardisiapusilla. A total of 88,444 unigenes were identified by RNA-seq analysis, of which 49,623 were successfully annotated and 4101 were differentially expressed. Gene ontology analysis revealed several subcategories of genes related to toluene response, including cell part, cellular process, organelle, and metabolic processes. We mapped the main metabolic pathways of genes related to toluene response and found that the differentially expressed genes were mainly involved in glycolysis/gluconeogenesis, starch and sucrose metabolism, glycerophospholipid metabolism, carotenoid biosynthesis, phenylpropanoid biosynthesis, and flavonoid biosynthesis. In addition, 53 transcription factors belonging to 13 transcription factor families were identified. We verified 10 differentially expressed genes related to metabolic pathways using quantitative real-time PCR and found that the results of RNA-seq were positively correlated with them, indicating that the transcriptome data were reliable. This study provides insights into the metabolic pathways involved in toluene response in plants.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%