Enhanced Detoxification of Arsenic Under Carbon Starvation: A New Insight into Microbial Arsenic Physiology

Nandre, Vinod S.; Bachate, Sachin P.; Salunkhe, Rahul C.; Bagade, Aditi V.; Shouche, Yogesh S.; Kodam, Kisan M.

doi:10.1007/s00284-017-1203-4

Cited by 20 publications

(9 citation statements)

References 35 publications

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“…In H. arsenicoxydans, the expression of genes in the aio operon is related to the presence of AsIII and to the expression of genes involved in motility [38]. Our results are in accordance with those of Nandre et al [24], who observed a higher expression of aioA genes under acetate starvation condition in seven strains belonging to different genera, for one single time of incubation (8 h), in presence of 1.33 mM of As. Here, the phenomenon was observed with a complex organic substrate (yeast extract) and correlated with early growth phase.…”

Section: Discussionsupporting

confidence: 92%

“…Several hypotheses may explain the negative effect of organic substrates on aioA gene expression and the bacterial specific AsIII-oxidation rate. Nandre et al [24] hypothesized cross protection in the carbon starvation stress wherein nutrient deprived cells show higher resistance against other stresses. However, this phenomenon could hardly explain results obtained with bacteria such as "T. arsenivorans" usually growing in mineral or oligotrophic media.…”

Section: Discussionmentioning

confidence: 99%

“…It therefore appears that organic substrates slow down the transformation of AsIII into AsV by bacteria in aerated conditions, and may decrease the retention of As in the environment. Nandre et al [24] showed that the presence of acetate in the growth medium induced a lower aioA gene expression in diverse bacterial strains isolated from tannery effluent and soils. Organic substrates may inhibit the aio encoded oxidation processes, because bacteria would preferentially metabolize simple or complex organic substrates providing more energy than AsIII.…”

Section: Introductionmentioning

confidence: 99%

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Simple or complex organic substrates inhibit arsenite oxidation and aioA gene expression in two β-Proteobacteria strains

Lescure

Joulian

Charles

et al. 2020

Research in Microbiology

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simple or complex organic substrates inhibit arsenite oxidation and aioA gene expression in two β-Proteobacteria strains

Lescure

Joulian

Charles

et al. 2020

Research in Microbiology

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“…In our study, the addition of 3-/4-HBA delayed As(III) oxidation by strain H7. It has been reported that bacterial As(III) oxidation is related to the available carbon sources and that carbon starvation could enhance bacterial As(III) oxidation (Nandre et al, 2017; Lu et al, 2018). 1/10 ST medium is a nutrient poor medium, and 3-HBA and 4-HBA could serve as carbon sources.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous 3-/4-Hydroxybenzoates Biodegradation and Arsenite Oxidation by Hydrogenophaga sp. H7

et al. 2019

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Aromatic compounds and arsenic (As) often coexist in the environment. As(III)-oxidizing bacteria can oxidize the more toxic As(III) into the less toxic As(V), and As(V) is easily removed. Microorganisms with the ability to degrade aromatic compounds and oxidize arsenite [As(III)] may have strong potential to remediate co-contaminated water. In this study, a Gram-negative bacterium Hydrogenophaga sp. H7 was shown to simultaneously degrade 3-hydroxybenzoate (3-HBA) or 4-HBA (3-/4-HBA) and oxidize arsenite [As(III)] to arsenate [As(V)] during culture. Notably, the addition of As(III) enhanced the degradation rates of 3-/4-HBA, while the addition of 3-/4-HBA resulted in a slight delay in As(III) oxidation. Use of a 1% bacterial culture in combination with FeCl 3 could completely degrade 250 mg/L 3-HBA or 4-HBA and remove 400 μM As(III) from simulated lake water within 28 h. Genomic analysis revealed the presence of As(III) oxidation/resistance genes and two putative 3-/4-HBA degradation pathways (the protocatechuate 4,5-dioxygenase degradation pathway and the catechol 2,3-dioxygenase degradation pathway). Comparative proteomics suggested that strain H7 degraded 4-HBA via the protocatechuate 4,5-dioxygenase degradation pathway in the absence of As(III); however, 4-HBA could be degraded via the catechol 2,3-dioxygenase degradation pathway in the presence of As(III). In the presence of As(III), more NADH was produced by the catechol 2,3-dioxygenase degradation pathway and/or by As(III) oxidation, which explained the enhancement of bacterial 4-HBA degradation in the presence of As(III). In addition, the key gene dmpB , which encodes catechol 2,3-dioxygenase in the catechol 2,3-dioxygenase degradation pathway, was knocked out, which resulted in the disappearance of As(III)-enhanced bacterial 4-HBA degradation from the dmpB mutant strain, which further confirmed that As(III) enhancement of 4-HBA degradation was due to the utilization of the catechol 2,3-dioxygenase pathway. These discoveries indicate that Hydrogenophaga sp. H7 has promise for the application to the removal of aromatic compounds and As co-contamination and reveal the relationship between As oxidation and aromatic compound degradation.

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“…Once arsenic enters into the human body, the skin, the cardiovascular systems, the nervous system, the kidney and the liver will be injured significantly. When the concentration of arsenic in the human body is 0.01–0.052 g, it will lead to arsenic poisoning; when the concentration of arsenic is 0.06–0.2 g, it will lead to death (Zeng et al 2017 ; Nandre et al 2017 ; Mukherjee et al 2017 ; Kempahanumakkagari et al 2017 ). Arsenic was considered as the priority controlled pollutants by World Health Organization (WHO) (El-Moselhy et al 2017 ; Miroslav et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Study on the removal and transport and migration mechanism for As with activated sludge system

et al. 2017

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In this paper, the removal of As and the transport and migration of As at the process of activated sludge system was studied. The results showed that the activated sludge system has high removal efficiency for As, and the removal efficiency could be nearly 100%. The initial concentration of As has effect on the removal efficiency, and within the experimental scope, the increase of initial concentration of As improved the removal efficiency for As. The distribution of As in the surface and internal in activated sludge indicates that in the process of activated sludge, the As was first transferred from water to the surface of solid and was adsorbed by the surface and then transport to the interior of microbes and take part in some metabolisms of the microorganisms. The adsorption of As by activated sludge conforms to Freundlich adsorption isotherm, and the removal of As in activated sludge system follows to the pseudo second order reaction kinetics.

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Enhanced Detoxification of Arsenic Under Carbon Starvation: A New Insight into Microbial Arsenic Physiology

Cited by 20 publications

References 35 publications

Simple or complex organic substrates inhibit arsenite oxidation and aioA gene expression in two β-Proteobacteria strains

Simple or complex organic substrates inhibit arsenite oxidation and aioA gene expression in two β-Proteobacteria strains

Simultaneous 3-/4-Hydroxybenzoates Biodegradation and Arsenite Oxidation by Hydrogenophaga sp. H7

Study on the removal and transport and migration mechanism for As with activated sludge system

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