Enhanced delineation of white matter structures of the fixed mouse brain using Gd‐DTPA in microscopic MRI

Kim, Sungheon; Pickup, Stephen; Hsu, Oliver; Poptani, Harish

doi:10.1002/nbm.1324

Cited by 29 publications

(22 citation statements)

References 32 publications

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“…Accordingly, the signal-to-noise ratio and hence image quality is diminished. To increase the CNR, numerous studies have successfully employed gadolinium-based contrast agents (Kim et al, 2008;Petiet et al, 2007;Spencer et al, 2006). Gadolinium alters the relaxation times of the water protons, thereby allowing for the identification of a greater number of anatomical structures.…”

Section: Resultsmentioning

confidence: 99%

A three-dimensional digital atlas of the zebrafish brain

et al. 2010

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Section: Resultsmentioning

confidence: 99%

A three-dimensional digital atlas of the zebrafish brain

et al. 2010

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“…These deposits have typical dimensions of less than 100 mm. Furthermore, MR microscopy in fixed mouse specimen has been applied for assessing minute alterations in anatomy (5,6) or for structural phenotyping of animal models of human diseases (7,8).…”

Section: Introductionmentioning

confidence: 99%

Micro MRI of the mouse brain using a novel 400 MHz cryogenic quadrature RF probe

et al. 2009

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The increasing number of mouse models of human disease used in biomedical research applications has led to an enhanced interest in non-invasive imaging of mice, e.g. using MRI for phenotyping. However, MRI of small rodents puts high demands on the sensitivity of data acquisition. This requirement can be addressed by using cryogenic radio-frequency (RF) detection devices. The aim of this work was to investigate the in vivo performance of a 400 MHz cryogenic transmit/receive RF probe (CryoProbe) designed for MRI of the mouse brain. To characterize this novel probe, MR data sets were acquired with both the CryoProbe and a matched conventional receive-only surface coil operating at room temperature (RT) using conventional acquisition protocols (gradient and spin echo) with identical parameter settings. Quantitative comparisons in phantom and in vivo experiments revealed gains in the signal-to-noise ratio (SNR) of 2.4 and 2.5, respectively. The increased sensitivity of the CryoProbe was invested to enhance the image quality of high resolution structural images acquired in scan times compatible with routine operation (< 45 min). In high resolution (30 x 30 x 300 microm(3)) structural images of the mouse cerebellum, anatomical details such as Purkinje cell and molecular layers could be identified. Similarly, isotropic (60 x 60 x 60 microm(3)) imaging of mouse cortical and subcortical areas revealed anatomical structures smaller than 100 microm. Finally, 3D MR angiography (52 x 80 x 80 microm(3)) of the brain vasculature enabled the detailed reconstruction of intracranial vessels (anterior and middle cerebral artery). In conclusion, this low temperature detection device represents an attractive option to increase the performance of small animal MR systems operating at 9.4 Tesla.

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“…7(b)], white and black stripes correspond to cerebellar white matter and the granular layer, respectively. 55,56 The cerebellar white matter, made up largely of myelinated nerve fibers, has high concentration of lipid, and the granular layer has a high concentration of cytochrome. We calculated the absorption ratio (AR) between the granular cell layer and the cerebellar white matter as follows:…”

Section: Discussionmentioning

confidence: 99%

Label-free photoacoustic tomography of whole mouse brain structures ex vivo

Xia

et al. 2016

Neurophoton.

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Abstract. Capitalizing on endogenous hemoglobin contrast, photoacoustic-computed tomography (PACT), a deep-tissue high-resolution imaging modality, has drawn increasing interest in neuroimaging. However, most existing studies are limited to functional imaging on the cortical surface and the deep brain structural imaging capability of PACT has never been demonstrated. Here, we explicitly studied the limiting factors of deep brain PACT imaging. We found that the skull distorted the acoustic signal and blood suppressed the structural contrast from other chromophores. When the two effects are mitigated, PACT can potentially provide high-resolution label-free imaging of structures in the entire mouse brain. With 100-μm in-plane resolution, we can clearly identify major structures of the brain, which complements magnetic resonance microscopy for imaging small-animal brain structures. Spectral PACT studies indicate that structural contrasts mainly originate from cytochrome distribution and that the presence of lipid sharpens the image contrast; brain histology results provide further validation. The feasibility of imaging the structure of the brain in vivo is also discussed. Our results demonstrate that PACT is a promising modality for both structural and functional brain imaging.

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Enhanced delineation of white matter structures of the fixed mouse brain using Gd‐DTPA in microscopic MRI

Cited by 29 publications

References 32 publications

A three-dimensional digital atlas of the zebrafish brain

A three-dimensional digital atlas of the zebrafish brain

Micro MRI of the mouse brain using a novel 400 MHz cryogenic quadrature RF probe

Label-free photoacoustic tomography of whole mouse brain structures ex vivo

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