Enhanced Degradation of Diesel in the Rhizosphere of <i>Lupinus luteus</i>
 after Inoculation with Diesel-Degrading and Plant Growth-Promoting Bacterial Strains

Balseiro-Romero, María; Gkorezis, Panagiotis; Kidd, Petra; Vangronsveld, Jaco; Monterroso, C.

doi:10.2134/jeq2015.09.0465

Cited by 37 publications

(19 citation statements)

References 39 publications

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“…Besides, the presence of plant growth-promoting genes spread throughout its genome (Panagiotis Gkorezis, personal communication, February 2017) corroborates once more the potential of this strain to be used in association with plants in phytoremediation. Indeed, the performance of this strain has been recently tested in a rhizodegradation pot experiment using diesel-contaminated soil, and it significantly increased the degradation of diesel, specially of the longest alkanes (from C 19 ) [38], which is consistent with the results of this work. This indicated the need for further study in gene expression coding for metabolic pathways in order to more adequately select the most efficient strains to be applied in bioremediation scenarios.…”

Section: Discussionsupporting

confidence: 89%

Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

Balseiro-Romero

Gkorezis

Kidd

et al. 2017

International Journal of Phytoremediation

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Bioremediation of polluted soils is a promising technique with low environmental impact, which uses soil organisms to degrade soil contaminants. In this study, 19 bacterial strains isolated from a diesel-contaminated soil were screened for their diesel-degrading potential, biosurfactant (BS) production, and biofilm formation abilities, all desirable characteristics when selecting strains for re-inoculation into hydrocarbon-contaminated soils. Diesel-degradation rates were determined in vitro in minimal medium with diesel as the sole carbon source. The capacity to degrade diesel range organics (DROs) of strains SPG23 (Arthobacter sp.) and PF1 (Acinetobacter oleivorans) reached 17-26% of total DROs after 10 days, and 90% for strain GK2 (Acinetobacter calcoaceticus). The amount and rate of alkane degradation decreased significantly with increasing carbon number for strains SPG23 and PF1. Strain GK2, which produced BSs and biofilms, exhibited a greater extent, and faster rate of alkane degradation compared to SPG23 and PF1. Based on the outcomes of degradation experiments, in addition to BS production, biofilm formation capacities, and previous genome characterizations, strain GK2 is a promising candidate for microbial-assisted phytoremediation of diesel-contaminated soils. These results are of particular interest to select suitable strains for bioremediation, not only presenting high diesel-degradation rates, but also other characteristics which could improve rhizosphere colonization.

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Section: Discussionsupporting

confidence: 89%

Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

Balseiro-Romero

Gkorezis

Kidd

et al. 2017

International Journal of Phytoremediation

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“…With respect to the application of plant growth-promoting and PHC - degrading endophytes, a number of recent studies has identified bacterial isolates that may be useful inoculants to stimulate phytoremediation of PHC contaminated sites (Kukla et al, 2014; Tara et al, 2014; Zhang et al, 2014; Pawlik and Piotrowska-Seget, 2015; Balseiro-Romero et al, 2016). …”

Section: Endophytic Bacteria and Phc Remediationmentioning

confidence: 99%

The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective

et al. 2016

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Widespread pollution of terrestrial ecosystems with petroleum hydrocarbons (PHCs) has generated a need for remediation and, given that many PHCs are biodegradable, bio- and phyto-remediation are often viable approaches for active and passive remediation. This review focuses on phytoremediation with particular interest on the interactions between and use of plant-associated bacteria to restore PHC polluted sites. Plant-associated bacteria include endophytic, phyllospheric, and rhizospheric bacteria, and cooperation between these bacteria and their host plants allows for greater plant survivability and treatment outcomes in contaminated sites. Bacterially driven PHC bioremediation is attributed to the presence of diverse suites of metabolic genes for aliphatic and aromatic hydrocarbons, along with a broader suite of physiological properties including biosurfactant production, biofilm formation, chemotaxis to hydrocarbons, and flexibility in cell-surface hydrophobicity. In soils impacted by PHC contamination, microbial bioremediation generally relies on the addition of high-energy electron acceptors (e.g., oxygen) and fertilization to supply limiting nutrients (e.g., nitrogen, phosphorous, potassium) in the face of excess PHC carbon. As an alternative, the addition of plants can greatly improve bioremediation rates and outcomes as plants provide microbial habitats, improve soil porosity (thereby increasing mass transfer of substrates and electron acceptors), and exchange limiting nutrients with their microbial counterparts. In return, plant-associated microorganisms improve plant growth by reducing soil toxicity through contaminant removal, producing plant growth promoting metabolites, liberating sequestered plant nutrients from soil, fixing nitrogen, and more generally establishing the foundations of soil nutrient cycling. In a practical and applied sense, the collective action of plants and their associated microorganisms is advantageous for remediation of PHC contaminated soil in terms of overall cost and success rates for in situ implementation in a diversity of environments. Mechanistically, there remain biological unknowns that present challenges for applying bio- and phyto-remediation technologies without having a deep prior understanding of individual target sites. In this review, evidence from traditional and modern omics technologies is discussed to provide a framework for plant–microbe interactions during PHC remediation. The potential for integrating multiple molecular and computational techniques to evaluate linkages between microbial communities, plant communities and ecosystem processes is explored with an eye on improving phytoremediation of PHC contaminated sites.

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“…The combination of RP92+105 strains was used for inoculation in greenhouse experiments with diesel-contaminated soils due to the excellent results obtained in perlite experiments: shoot and root weight of L. luteus plants inoculated with RP92+105 increased by 50 % compared to the NI control ( Figure 2). This plant was selected for contaminated-soil experiments due to its fast growth and contaminant tolerance (Balseiro-Romero et al, 2016;Weyens et al, 2010).…”

Section: Plant Growth Nutritional Status and Stress-related Enzyme Amentioning

confidence: 99%

Use of plant growth promoting bacterial strains to improve Cytisus striatus and Lupinus luteus development for potential application in phytoremediation

Balseiro-Romero

Gkorezis

Kidd

et al. 2017

Science of The Total Environment

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Plant growth promoting (PGP) bacterial strains possess different mechanisms to improve plant development under common environmental stresses, and are therefore often used as inoculants in soil phytoremediation processes. The aims of the present work were to study the effects of a collection of plant growth promoting bacterial strains on plant development, antioxidant enzyme activities and nutritional status of Cytisus striatus and/or Lupinus luteus plants a) growing in perlite under non-stress conditions and b) growing in diesel-contaminated soil. For this, two greenhouse experiments were designed. Firstly, C. striatus and L. luteus plants were grown from seeds in perlite, and periodically inoculated with 6 PGP strains, either individually or in pairs. Secondly, L. luteus seedlings were grown in soil samples of the A and B horizons of a Cambisol contaminated with 1.25% (w/w) of diesel and inoculated with best PGP inoculant selected from the first experiment. The results indicated that the PGP strains tested in perlite significantly improved plant growth. Combination treatments provoked better growth of L. luteus than the respective individual strains, while individual inoculation treatments were more effective for C. striatus. L. luteus growth in diesel-contaminated soil was significantly improved in the presence of PGP strains, presenting a 2-fold or higher increase in plant biomass. Inoculants did not provoke significant changes in plant nutritional status, with the exception of a subset of siderophore-producing and P-solubilising bacterial strains that resulted in significantly modification of Fe or P concentrations in leaf tissues. Inoculants did not cause significant changes in enzyme activities in perlite experiments, however they significantly reduced oxidative stress in contaminated soils suggesting an improvement in plant tolerance to diesel. Some strains were applied to non-host plants, indicating a non-specific performance of their plant growth promotion. The use of PGP strains in phytoremediation may help plants to overcome contaminant and other soil stresses, increasing phytoremediation efficiency.

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Enhanced Degradation of Diesel in the Rhizosphere of Lupinus luteus after Inoculation with Diesel-Degrading and Plant Growth-Promoting Bacterial Strains

Cited by 37 publications

References 39 publications

Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation

The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective

Use of plant growth promoting bacterial strains to improve Cytisus striatus and Lupinus luteus development for potential application in phytoremediation

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