Enhanced cytotoxicity and nuclear accumulation of doxorubicin-loaded nanospheres in human breast cancer MCF7 cells expressing MRP1

Aouali, Nasséra; Morjani, Hamid; Trussardi, Aurélie; Soma, Émilienne; Giroux, B.; Manfait, Michel

doi:10.3892/ijo.23.4.1195

Cited by 11 publications

(10 citation statements)

References 16 publications

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“…[16] Based on IC 50 value, it was observed that tiliroside has a low cytotoxic activity on MCF7 cell lines. MCF-7 cancer cell lines are characterized by resistance chemotherapeutic agents, [17,18] responsive estrogen receptors (ER+), overexpression of Bcl-2, [19,20] and with no expression of Caspase 3. [21,22] Stimulation of ER could increase P-gp concentration.…”

Section: Cytotoxic Activity Assessment Of Tiliroside On T47d and Mcf-mentioning

confidence: 99%

Antiproliferative properties of tiliroside from Guazuma ulmifolia lamk on T47D and MCF7 cancer cell lines

Da'i¹,

Wikantyasning²,

Wahyuni³

et al. 2016

Natl J Physiol Pharm Pharmacol

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Background: Previous research showed that the extract of guazuma leaves had cytotoxic activity on various cancer cell lines. One of the chemical markers of Guazuma ulmifolia Lamk. is tiliroside. Aims and Objectives: The aim and objective of this study was to determine the cytotoxic effects of tiliroside on T47D and MCF7 and their mechanisms. Materials and Methods: Tiliroside (was isolated by laboratory Biological Pharmacy of Universitas Muhammadiyah Surakarta, was confirmed by using liquid chromatography-tandem mass spectrometry/MS). The antiproliferative activity was analyzed by flow cytometry and immunocytochemistry methods. Results: Tiliroside has cytotoxic activity with inhibitory concentration 50% on T47D and MCF7 which was 67.79 and 100.00 µg/ml, respectively. Based on the immunocytochemistry, tiliroside on T47D cell lines induced apoptosis which is mediated by the activation of Caspases 8 and 9 and decreased expression of Bcl-2 protein without interference with gen protein 53 (p53). In line with the previous one, tiliroside on MCF7 cell lines induced apoptosis through extrinsic ways due to the underexpression of p53 and Caspase 9 and increase in the expression of Bcl-2 and Caspase 8. Conclusions: The antiproliferative effects of tiliroside are higher on T47D than MCF7 cancers cell lines. Based on molecular analysis, antiproliferative mechanism was by apoptosis through extrinsic pathways.

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Section: Cytotoxic Activity Assessment Of Tiliroside On T47d and Mcf-mentioning

confidence: 99%

Antiproliferative properties of tiliroside from Guazuma ulmifolia lamk on T47D and MCF7 cancer cell lines

Da'i¹,

Wikantyasning²,

Wahyuni³

et al. 2016

Natl J Physiol Pharm Pharmacol

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“…Preclinical studies have shown that poly(isohexylcyanoacrylate) (PIHCA) nanoparticles, used as Dox carriers, were able to reduce cardiotoxicity by modifying tissue distribution (Kattan et al 1992). Dox, incorporated into PACA nanoparticles, has been investigated in different resistant cell lines (Soma et al 1999(Soma et al , 2000aAquali et al 2003). The potential of PACA nanoparticles to overcome multidrug resistance phenomena occurred at both cellular and non-cellular level has been summarized by Vauthier et al (2003).…”

Section: Introductionmentioning

confidence: 97%

“…Colloidal drug carriers such as poly(alkylcyanoacrylate) (PACA) nanoparticles have been proposed to overcome the two main problems, cumulative cardiotoxicity and acquired resistance, which limit the clinical application of anthracyclines (Kattan et al 1992;Soma et al 1999Soma et al , 2000aAquali et al 2003;Vauthier et al 2003). Preclinical studies have shown that poly(isohexylcyanoacrylate) (PIHCA) nanoparticles, used as Dox carriers, were able to reduce cardiotoxicity by modifying tissue distribution (Kattan et al 1992).…”

Section: Introductionmentioning

confidence: 97%

Effect of farmorubicin both free and associated with poly(butylcyanoacrylate) nanoparticles on phagocytic and NK activity of peritoneal exudate cells from tumor-bearing mice

Simeonova

Antcheva²

2007

Journal of Drug Targeting

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The effect of Epirubicin (farmorubicin, FR), either free or associated with poly(butylcyanoacrylate) nanoparticles (PBCN) upon the phagocytic and natural killer (NK) activity of peritoneal exudate cells (PECs) harvested from Lewis lung carcinoma (LLC)-bearing-mice was investigated. Phagocytic and NK activity were tested 72 and 96 h, respectively after the last four intraperitoneal (i.p.) injections of the tested compounds have been administered to the mice. Phagocytic activity was evaluated in vitro by phagocytic index and ingestion capacity using a phagocytic assay. NK activity was evaluated in a direct cytotoxic test, in which PECs were used as effector cells while human erythroleukemic K-562 cells were used as target cells. The phagocytic activity of PECs, harvested from tumor-bearing mice, was stimulated after treatment with FR free, FR associated with polymer nanoparticles and with unloaded PBCN. The NK activity of PECs was strongly stimulated by unloaded PBCN. FR both free and encapsulated into the polymer matrix during the polymerization of n-butylcyanoacrylate (n-BCA) stimulated the NK activity of PECs, while FR adsorbed onto nanoparticles restrained it. These results suggest that the association of FR with nanoparticles modifies selectively its immunomodulating ability without producing any significant immunological disturbances. The toxicity of some of FR polymer forms towards PECs, displaying NK activity, probably comes from the enhanced local drug concentration on the membrane surface of the immune cells. However, it is insufficient to preclude the use of nanoparticles as drug delivery system.

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“…For MRP1 expression, the monoclonal antibody MRPm6 (2.5 mg/ml) was used as described earlier. 29 Antibody binding was detected with the F(ab 0 )2 fragment of goat anti-mouse IgG fluorescein-FITC. Protein expression was determined as ratio of mean fluorescence of monoclonal antibodies UIC2/IgG2a.…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

Overcoming MDR-associated chemoresistance in HL-60 acute myeloid leukemia cells by targeting shingosine kinase-1

et al. 2005

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We examined the involvement of sphingosine kinase-1, a critical regulator of the sphingolipid balance, in susceptibility to antineoplastic agents of either sensitive or multidrugresistant acute myeloid leukemia cells. Contrary to parental HL-60 cells, doxorubicin and etoposide failed to trigger apoptosis in chemoresistant HL-60/Doxo and HL-60/VP16 cells overexpressing MRP1 and MDR1, respectively. Chemosensitive HL-60 cells displayed sphingosine kinase-1 inhibition coupled with ceramide generation. In contrast, chemoresistant HL-60/ Doxo and HL-60/VP16 had sustained sphingosine kinase-1 activity and did not produce ceramide during treatment. Enforced expression of sphingosine kinase-1 in chemosensitive HL-60 cells resulted in marked inhibition of apoptosis that was mediated by blockade of mitochondrial cytochrome c efflux hence suggesting a control of apoptosis at the premitochondrial level. Incubation with cell-permeable ceramide of chemoresistant cells led to a sphingosine kinase-1 inhibition and apoptosis both prevented by sphingosine kinase-1 overexpression. Furthermore, F-12509a, a new sphingosine kinase inhibitor, led to ceramide accumulation, decrease in sphingosine 1-phosphate content and caused apoptosis equally in chemosensitive and chemoresistant cell lines that is inhibited by adding sphingosine 1-phosphate or overexpressing sphingosine kinase-1. F-12509a induced classical apoptosis hallmarks namely nuclear fragmentation, caspase-3 cleavage as well as downregulation of antiapoptotic XIAP, and release of cytochrome c and SMAC/Diablo. Leukemia (2006) 20, 95-102.

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Enhanced cytotoxicity and nuclear accumulation of doxorubicin-loaded nanospheres in human breast cancer MCF7 cells expressing MRP1

Cited by 11 publications

References 16 publications

Antiproliferative properties of tiliroside from Guazuma ulmifolia lamk on T47D and MCF7 cancer cell lines

Antiproliferative properties of tiliroside from Guazuma ulmifolia lamk on T47D and MCF7 cancer cell lines

Effect of farmorubicin both free and associated with poly(butylcyanoacrylate) nanoparticles on phagocytic and NK activity of peritoneal exudate cells from tumor-bearing mice

Overcoming MDR-associated chemoresistance in HL-60 acute myeloid leukemia cells by targeting shingosine kinase-1

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