Enhanced cyanide biodegradation by immobilized crude extract of<i>Rhodococcus</i>UKMP-5M

Maniyam, Maegala Nallapan; Ibrahim, Ab Latif; Cass, Anthony E. G.

doi:10.1080/09593330.2017.1393015

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…Gellan gum is an ionic heteropolysaccharide. It is also believed able to be used repetitively up to 20 batches without substantial loss of catalytic activity [44]. This proves that the durability of the beads is above adequate.…”

Section: Reusability Of the Immobilized Beadsmentioning

confidence: 85%

“…In the study, immobilized Pseudomonas luteola with calcium alginate, k-carrageenan and polyacrylamide can be reused up to four times with 75, 85 and 80% of dye decolorization [43]. Another study has shown that the gellan gum immobilized beads were able to be reused up to 20 times without substantial loss of catalytic activity [44]. No data was available on the reusability of gellan gum immobilized cells in Metanil Yellow dye decolorization.…”

Section: Reusability Of the Immobilized Beadsmentioning

confidence: 99%

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Immobilization of Metanil Yellow Decolorizing Mixed Culture FN3 Using Gelling Gum as Matrix for Bioremediation Application

et al. 2020

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In this study, the Metanil Yellow (MY) decolorizing mixed culture, namely FN3, has been isolated from agriculture soil. The mixed culture was immobilized using gellan gum. In order to optimize the immobilization process for maximal dye decolorization, Response Surface Methodology (RSM) was performed. The optimal conditions for immobilization predicted by desirability function are 130 mg/L of MY dye concentration, 1.478% of gellan gum concentration, 50 beads and 0.6 cm of beads size with the percentage of decolorization of 90.378%. The correlation coefficients of the model (R2 and R2 adj) are 0.9767 and 0.9533, respectively. This indicates that the established model is suitable to predict the effectiveness of dye decolorization under the investigated condition. The immobilized beads of mixed culture FN3 were able to be reused up to 15 batches of decolorization. The immobilized cells also have high tolerance towards heavy metals. This was proven by higher dye decolorization rate by the immobilized cells even with the addition of heavy metals in the media. The decolorization potential of the mixed culture indicates that it could be useful for future bioremediation of soil contaminated sites and treatment solutions of water bodies polluted with MY dye.

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Section: Reusability Of the Immobilized Beadsmentioning

confidence: 85%

Section: Reusability Of the Immobilized Beadsmentioning

confidence: 99%

Immobilization of Metanil Yellow Decolorizing Mixed Culture FN3 Using Gelling Gum as Matrix for Bioremediation Application

et al. 2020

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“…Cell leakage as well as the free cell content immobilized on both support matrices after encapsulation were quantified by calculating the dry cell weight. Immobilization yield was determined following protocols described in [9] by substituting protein content with dry cell weight.…”

Section: G Decolourization Of Methylene Blue By Resting Cells and Immentioning

confidence: 99%

Decolourization of Methylene Blue by Rhodococcus Strain UCC 0003

Maniyam¹,

Seksyen²,

Sjahrir³

et al. 2018

IJESD

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Methylene blue is widely utilized as biological stains, in printing and as commercial textile dye. The increasing presence of textile dye in wastewater for instance represents environmental hazards. Therefore, there is a significant interest in developing cost effective and environmentally friendly methods for methylene blue removal from industrial wastewater. Taking this into consideration, in the present study, twenty three locally isolated Rhodococcus strains were examined as biological tools for decolourization of methylene blue. Among the tested microorganisms, five locally isolated Rhodococcus strains demonstrated promising ability to decolourize methylene blue as evidenced by the change in the colour of the dye from dark blue to pale blue on nutrient agar plates. One particular strain namely Rhodococcus strain UCC 0003 formed clear zone around the colonies of the bacteria with the biggest diameter of 2.0 ± 0.1 cm after 2 days of cultivation time. This strain demonstrated good growth and completely decolourized 0.1 g/L methylene blue after 5 days of incubation period. Secondary characterization was carried out by comparing the ability of resting cells and immobilized cells of Rhodococcus strain UCC 0003 for efficient methylene blue removal. The results showed that the highest percentage of methylene blue decolourization was achieved by using polyurethane foam cubes as the immobilization matrix resulting in 83 a ± 1 % compared to that of 63 b ± 3 % when resting cells was employed as the biocatalyst. These findings clearly indicated that the immobilized cells of Rhodococcus strain UCC 0003 has a huge potential as biological tool to remediate actual wastewater containing methylene blue.

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“…This immobilization matrix has better rheological characteristics in comparison to that of agar and carrageenan gels when assessed at similar concentrations [8]. Besides, the hydrogels are able to sustain stability over a broad pH range which is from 2 to 10 [9]. Gellan gum can be used as a simple matrix for enzyme immobilization due to its polyelectrolyte property, hydrophilicity, emulsification attributes, its non-hazardous features, biocompatibility and biodegradability [7].…”

Section: Introductionmentioning

confidence: 99%

Conversion of waste cooking oil by rhodococcal lipase immobilized in gellan gum

et al. 2021

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Recently, the application of lipase enzyme as biocatalyst in the conversion of waste cooking oil (WCO) to free fatty acids and glycerol has been trending well. Therefore, the present study attempts to use WCO which is found in abundance in Malaysia as the substrate for halal microbial lipase conversion to glycerol which can be exploited in the food industry. The workability of free lipase for WCO conversion, however suffers severely due to potential denaturation of the enzyme and extended reaction time. Thus, this study embraced the immobilization method to encapsulate crude lipase extracted from Rhodococcus pyridinivorans strain UCC 0009 in gellan gum and calcium alginate, respectively and compared their ability for WCO conversion to free crude lipase. The gellan gum and calcium alginate-immobilized crude lipase evidently exhibited greater WCO conversion, demonstrating 2.18-fold and 1.61-fold enhanced lipase activity, respectively in comparison to free crude lipase. The repeated reuse of the gellan gum-immobilized crude lipase maintained reasonable lipase activity for 9 cycles, retaining an average 85 % WCO conversion for the first seven cycles and 67 % conversion in the subsequent batches. Thus, the immobilized halal lipase can be foreseen as a green substitute to chemical catalyst for WCO conversion which meets the worldwide demand for clean technologies.

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Enhanced cyanide biodegradation by immobilized crude extract ofRhodococcusUKMP-5M

Cited by 11 publications

References 30 publications

Immobilization of Metanil Yellow Decolorizing Mixed Culture FN3 Using Gelling Gum as Matrix for Bioremediation Application

Immobilization of Metanil Yellow Decolorizing Mixed Culture FN3 Using Gelling Gum as Matrix for Bioremediation Application

Decolourization of Methylene Blue by Rhodococcus Strain UCC 0003

Conversion of waste cooking oil by rhodococcal lipase immobilized in gellan gum

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