Enhanced cutinase production with Thermobifida fusca by two‐stage pH control strategy

Abstract

A mutant of Thermobifida fusca ATCC 27730 was used for cutinase production. Acetate was the most suitable carbon source for cell growth and cutinase production compared with others. The pH was one of the most important factors affecting cutinase yield and productivity. Batch cutinase fermentations by mutant Thermobifida fusca WSH04 at various pH values ranging from 7.0 to 7.9 were studied. Based on the effects of different pH values on the specific cell growth rate and specific cutinase formation rate, a two-stage pH control strategy was developed, in which the pH was set at 7.3 for the first 20 h, and switched to 7.6 afterwards. By applying this two-stage pH control strategy for cutinase fermentation, the maximal cutinase activity reached 19.8 U/mL.

Modeling and optimization of cutinase production by recombinant Escherichia coli based on statistical experimental designs

“…In our previous work, the microbial cutinase by Thermobifida fusca was produced and the highest cutinase activity was 51 U/mL. 5 The results obtained here revealed that the recombinant E. coli was an ideal host for the recombinant cutinase production.…”

“…The cutinase concentrations shown are the mean values of triplicate experiments. The values of the regression coefficient were calculated and the first-order model could be written from the coefficients: y cutinase =89.01+5.62x 1 +15.79x 2 +6.98x 3 +1.08x 4 +0.50x 5 (3)…”

“…In our previous work, significant enhancement of cutinase production by Thermobifida fusca was achieved with two-stage pH control [5] and two-stage batch and fed-batch cultivation [21]. After optimization of culture conditions, the maximal cutinase activity reached 51.0 U/mL.…”

“…Recently, applications of cutinase in the textile industry have also gained considerable attention [1,6]. Compared with traditional techniques in cotton desizing, cutinase improves cotton wettability and fabric hydrophilicity in addition to its ability to simplify processes, reduce working time, save production cost, and alleviate environmental pollution [4,5].…”

“…Cutinases display hydrolytic activity on soluble synthetic esters and insoluble long-chain triacylglycerols, and perform transesterification of fats and oils and (stereo) selective esterification of alcohols [3]. Therefore, cutinases have great potential applications in the dairy industry for the hydrolysis of milk fat, in household detergents, in the oleochemical industry, in the synthesis of structured triglycerides, polymers and surfactants, ingredients for personal-care products, and pharmaceuticals [4,5]. Recently, applications of cutinase in the textile industry have also gained considerable attention [1,6].…”