Enhanced cellulolytic enzyme production by the synergism between Trichoderma reesei RUT-C30 and Aspergillus niger NL02 and by the addition of surfactants

Fang, Hao; Zhao, Chen; Song, Xiangyang; Chen, Mu; Zheng, Chao; Chu, Jianzhou

doi:10.1007/s12257-012-0562-8

Cited by 46 publications

(29 citation statements)

References 40 publications

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“…This observation indicates that T. reesei cellulase lacks of cellobiase and xylanase. It is well known that T. reesei is deficient in cellobiase, which can be solved by fermentation or genetic engineering techniques [5,6,8]. In all cases in Fig.…”

Section: Enzymatic Hydrolysis Of Shpcs and Shprsmentioning

confidence: 99%

Heterologous expression and production of Trichoderma reesei cellobiohydrolase II in Pichia pastoris and the application in the enzymatic hydrolysis of corn stover and rice straw

Fang

Xia

2015

Biomass and Bioenergy

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Section: Enzymatic Hydrolysis Of Shpcs and Shprsmentioning

confidence: 99%

Heterologous expression and production of Trichoderma reesei cellobiohydrolase II in Pichia pastoris and the application in the enzymatic hydrolysis of corn stover and rice straw

Fang

Xia

2015

Biomass and Bioenergy

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“…Though some of them had been compared in the enzymatic hydrolysis of corn stover, 11 it's the rst we compared them in the SCO production by M. isabellina from cron stover. On-site cellulase production by the mixed culture of Trichoderma reesei and Aspergillus niger 6,12,13 was carried out to realize cellulase autarky due to its advantages as described by Culbertson et al…”

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confidence: 99%

Microbial oil production byMortierella isabellinafrom corn stover under different pretreatments

et al. 2017

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Mixed culture of Trichoderma reesei and Aspergillus niger was employed to accomplish on-site cellulase production where cellulases were applied directly to the enzymatic hydrolysis of pretreated corn stover. We comprehensively compared the five pretreatments including sodium hydroxide, steam explosion, aqueous ammonia, lime and diluted sulfuric acid in the enzymatic hydrolysis and then in the whole processes from corn stover to single cell oil (SCO). The results were not completely but roughly the same. However, it is conclusive that sodium hydroxide pretreatment was the best one. The process with sodium hydroxide pretreatment could produce 23.5 g dry cell biomass harboring 13.7 g single cell oil (SCO) from 146.2 g corn stover. The total yields of cell biomass and SCO were 0.161 and 0.094 g g À1 corn stover, respectively. This process was proven the most efficient. Through this work, we established efficient SCO production process from corn stover.

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“…To overcome this problem, synergistic fermentation could be used for cellulose fermentation to complement the enzyme systems and increase the capacity of cellulose hydrolysis. Synergistic fermentation of A. niger with T. reesei could compensate for the lack of β-glucosidase of T. reesei to some degree (Muthuvelayudham and Viruthagiri 2007;Fang et al 2013). The predominant cellulolytic fungi in subtropical and tropical forests of China are Trichoderma and Penicillium (Zhang et al 2014).…”

Section: Reducing Sugar Concentration and Cellulase Activity Of Synermentioning

confidence: 99%

Synergistic Fermentation of Camphor Leaves by Cellulolytic Fungus and Optimization of Reducing Sugar Production

Zeng

Chen

et al. 2017

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The degradation of lignocellulosic materials requires the synergy of multiple enzymes. Synergistic fermentation would be an alternative to a complete enzyme system and consequentially enhance enzymatic production. The present study used cellulolytic fungus of Penicillium decumbens, Aspergillus niger C112, and Trichoderma reesei RutC-30 as the starting strains, to screen for the best combination and proportion of synergistic fermentation. The experiments of different combinations and inoculum ratios for synergistic fermentation were performed, and BoxBehnken designs were adopted to optimize the fermentation conditions. The best combination of strains was confirmed as T. reesei RutC-30 and P. decumbens with an 8:2 inoculum ratio. The maximum sugar concentration was 1.48 g/L with CMCase (17.53 U/mL) and FPase (4.78 U/mL) in this situation. Response surface methodology revealed the optimized parameters as a substrate concentration of 5.93% at 32.12 °C, and a fermentation time of 5.85 d with a predicted sugar concentration of 2.91 g/L. Under these conditions, a maximum sugar production of 2.96 g/L was achieved in verification experiments.

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Enhanced cellulolytic enzyme production by the synergism between Trichoderma reesei RUT-C30 and Aspergillus niger NL02 and by the addition of surfactants

Cited by 46 publications

References 40 publications

Heterologous expression and production of Trichoderma reesei cellobiohydrolase II in Pichia pastoris and the application in the enzymatic hydrolysis of corn stover and rice straw

Heterologous expression and production of Trichoderma reesei cellobiohydrolase II in Pichia pastoris and the application in the enzymatic hydrolysis of corn stover and rice straw

Microbial oil production byMortierella isabellinafrom corn stover under different pretreatments

Synergistic Fermentation of Camphor Leaves by Cellulolytic Fungus and Optimization of Reducing Sugar Production

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