Enhanced cell functions on graphene oxide incorporated 3D printed polycaprolactone scaffolds

Unagolla, Janitha M.; Jayasuriya, Ambalangodage C.

doi:10.1016/j.msec.2019.04.026

Cited by 61 publications

(41 citation statements)

References 49 publications

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“…GDs could be introduced into different kinds of substrates, such as metals, [130][131][132] inorganic nonmetals, [133][134][135][136] natural polymers [137][138][139] and synthetic polymers [140][141][142][143] to prepare bone repair materials with improved performance, as shown in Table 3. In this subsection, the updated progress on research and development of those materials was reviewed.…”

Section: Composites With Different Substrates For Bone Repair Materialsmentioning

confidence: 99%

“…GDs could be uniformly dispersed into the polymer matrix by membrane lamination, fiber bonding, 3D printing and other methods to make up for these shortcomings. For example, Unagolla et al 140 prepared PCL-GO composite scaffolds by 3D printing, which could independently control the size and pore diameter of the implants. The morphology observation showed that the surface of the pure PCL scaffold was rough and irregular, while the surface of the scaffold containing GO was smooth and there were GO particles on the surface, which indicated that the introduction of GO improved the fluidity of the composites.…”

Section: Composites With Different Substrates For Bone Repair Materialsmentioning

confidence: 99%

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Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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Section: Composites With Different Substrates For Bone Repair Materialsmentioning

confidence: 99%

Section: Composites With Different Substrates For Bone Repair Materialsmentioning

confidence: 99%

Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects

Du¹,

Wang

Zhang³

et al. 2020

IJN

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“…The properties of alginate, i.e., flexibility, gelation time, and pore size, can be tuned to meet the bioprinting requirements by controlling the relative ratio between the two block components (mannuronic acid and glucuronic acid) and chiefly crosslinking glucuronic with divalent ions (Ca 2+ Sr 2+ , Ba 2+ ). Research efforts have been focused on reducing the alginate concentration in bioink, thus increasing the pore size and gas and nutrient exchange, thereby enhancing cell viability and proliferation [ 37 , 78 , 79 ].…”

Section: Cell-laden Bioinksmentioning

confidence: 99%

Overview of Current Advances in Extrusion Bioprinting for Skin Applications

Perez‐Valle

Amo

Andı́a

2020

IJMS

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Bioprinting technologies, which have the ability to combine various human cell phenotypes, signaling proteins, extracellular matrix components, and other scaffold-like biomaterials, are currently being exploited for the fabrication of human skin in regenerative medicine. We performed a systematic review to appraise the latest advances in 3D bioprinting for skin applications, describing the main cell phenotypes, signaling proteins, and bioinks used in extrusion platforms. To understand the current limitations of this technology for skin bioprinting, we briefly address the relevant aspects of skin biology. This field is in the early stage of development, and reported research on extrusion bioprinting for skin applications has shown moderate progress. We have identified two major trends. First, the biomimetic approach uses cell-laden natural polymers, including fibrinogen, decellularized extracellular matrix, and collagen. Second, the material engineering line of research, which is focused on the optimization of printable biomaterials that expedite the manufacturing process, mainly involves chemically functionalized polymers and reinforcement strategies through molecular blending and postprinting interventions, i.e., ionic, covalent, or light entanglement, to enhance the mechanical properties of the construct and facilitate layer-by-layer deposition. Skin constructs manufactured using the biomimetic approach have reached a higher level of complexity in biological terms, including up to five different cell phenotypes and mirroring the epidermis, dermis and hypodermis. The confluence of the two perspectives, representing interdisciplinary inputs, is required for further advancement toward the future translation of extrusion bioprinting and to meet the urgent clinical demand for skin equivalents.

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“…3,4 The absorbable polymer compound polycaprolactone (PCL) has excellent biocompatibility, degradability and strong mechanical strength and is widely used in clinical practice. 5,6 However, the material is single and has no biological activity so it is not conducive to cell adhesion and proliferation. Thus, the material should be modified.…”

Section: Introductionmentioning

confidence: 99%

Regulation of ERK1/2 and SMAD2/3 Pathways by Using Multi-Layered Electrospun PCL–Amnion Nanofibrous Membranes for the Prevention of Post-Surgical Tendon Adhesion

Liu¹,

Tian²,

Bai³

et al. 2020

IJN

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Background: Adhesion after tendon injury is a common complication in clinical practice. The lack of effective prevention mechanisms seriously affects the functional rehabilitation of patients. This research aimed to optimise the amniotic membrane and explain the mechanism of tendon-amniotic membrane by imitating the tendon sheath to construct a multilayer electrospun polycaprolactone (PCL) nanofibre membrane. Materials and Methods: Fresh amnions were subjected to freezing and vacuum drying. The two surfaces of freeze-dried amnions were coated with PCL nanofibres by electrospinning, thereby forming a multilayer composite membrane and constructing a growth factor-sustained release system conforming to the tendon-healing cycle. The new materials were characterised, and the biological effects on tenocytes and fibroblasts were evaluated. The tendon injury model of New Zealand rabbits was constructed to observe the effects on tendon adhesion and healing. Results: After freezing and vacuum drying, fresh amnions were found to effectively remove most of the cell components but retained the active components TGF-β1, bFGF, VEGF, and PDGF, as well as the fibrous reticular structure of the basement membrane. After coating with PCL nanofibres, a composite membrane mimicking the structure of the tendon sheath was constructed, thereby strengthening the tensile strength of the amnion. By up-regulating the phosphorylation of ERK1/2 and SMAD2/3, the adhesion and proliferation of tenocytes and fibroblasts were promoted, and collagen synthesis was enhanced. In the rabbit tendon repair model, the composite membrane effectively isolated the exogenous adhesion tissue and promoted endogenous tendon healing. Conclusion: The composite membrane mimicking the structure of tendon sheath effectively isolated the exogenous adhesion tissue and achieved good tendon slip. By slowly releasing the growth factors TGF-β1, bFGF, VEGF and PDGF, the ERK1/2 and SMAD2/3 pathways were regulated. Consequently, endogenous tendon healing was promoted. This strategy can alternatively address the clinical problem of tendon adhesion.

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Enhanced cell functions on graphene oxide incorporated 3D printed polycaprolactone scaffolds

Cited by 61 publications

References 49 publications

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Overview of Current Advances in Extrusion Bioprinting for Skin Applications

<p>Regulation of ERK1/2 and SMAD2/3 Pathways by Using Multi-Layered Electrospun PCL–Amnion Nanofibrous Membranes for the Prevention of Post-Surgical Tendon Adhesion</p>

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