Enhanced catharanthine production in catharanthus roseus cell cultures by combined elicitor treatment in shake flasks and bioreactors

Effect of Methyl Jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves

“…The catalase activity reduction was also registered in Catharanthus roseus cultures, induced by fungi (Zhao et al 2001) and by water stress in strawberry (Wang, 1999).…”

Enhanced production of silymarin by Ag+elicitor in cell suspension cultures ofSilybum marianum

“…Hairy root cultures of S. marianum produce silymarin. It has been observed that hairy roots of S. marianum are susceptible to elicitation by an elicitor, and yeast extract increased silybin production (Rahnama et al, 2008 , Ag + , and V, all stimulate the biosynthesis of a wide range of plant secondary metabolites (Neil et al, 1994;Tumova et al, 2001;Zhao et al, 2001). Therefore, we examined the effects of Ag on the growth and production of SLM in cell suspension cultures of S. marianum.…”

Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

“…This fact may be due to the induction of some sort of stress that does not involve an increase in the production of ajmalicine, while UV-C light exposure may enhance the ajmalicine production but the elicitation in the presence of CDs is needed to provoke any enhancement. Zhao et al, (2001) also observed a synergistic effect on indole alkaloid accumulation in C. roseus cell cultures elicited with fungal preparations and chemicals. Among them, the combination of tetramethyl ammonium bromide and Aspergillum niger mycelial homogenate gave a maximal ajmalicine production of 0.84 ± 0.05 mg/g DW and an improved catharanthine accumulation of 0.57 ± 0.04 mg/g DW, values that are below those obtained in the best production conditions described above.…”

“…Catharanthine can be chemically coupled with vindoline to form the clinically important anticancer drug, vinblastine and so this could provide a novel and efficient way to produce vinblastine commercially. Vindoline is abundant in C. roseus plants, and catharanthine can be produced by C. roseus cell or hairy root cultures (Zhao et al, 2001), so a combination of both pathways could lead to a very high vinblastine production. Therefore, the production of catharanthine by various C. roseus cell types in culture and its mechanism of biosynthesis has been one of most extensively explored areas of plant cell or hairy root cultures in recent years.…”