Enhanced bone regeneration <i>via</i> PHA scaffolds coated with polydopamine-captured BMP2

Zhang, Xu; Li, Jian; Chen, Jin; Peng, Zixin; Chen, Jiangnan; Liu, Xin-Yi; Wu, Fuqing; Zhang, Peng; Chen, George Guo-Qiang

doi:10.1039/d2tb01122k

Cited by 14 publications

(14 citation statements)

References 66 publications

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“…Currently, PHB/P34HB-BMP2/PDA materials that adhere to PDA under alkaline and aerobic conditions are being designed, resulting in improved osteogenic bioactivity and improved adhesion and proliferation of stem cells with prolonged release for 30 days via a bioactive pathway. 113 Researchers designed a material with mixtures of PHB/PHBHHx/ PLA/PEG, which were implanted subcutaneously in rabbits to evaluate their degradation and tissue reactions. The results of the in vivo tests demonstrated that all the materials tested provoked mild responses in the tissues without inducing any rejection of the material.…”

Section: Studiesmentioning

confidence: 99%

The role of poly(3‐hydroxybutyrate)‐based derivatives in skin, nerve, cartilage, and bone tissue engineering applications—An updated review

Reyna‐Urrutia,

Leyva‐Gómez,

Ríos

et al. 2024

Polymers for Advanced Techs

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Poly(3‐hydroxybutyrate) (PHB) has gained significant attention in the realm of medical applications in recent years. This acceptability is rooted in its bioactive properties and remarkable biocompatibility, as the degradation products exhibit negligible cytotoxicity. PHB boasts commendable mechanical resilience, distinguishing it from other polymers commonly used in tissue engineering. Using PHB in fabricating 3D scaffolds serves various objectives, often involving a combination with other polymers, ceramics, fibers, or regenerative factors. This distinctive approach sets it apart, leading to the creation of composite biomaterials. These incorporated materials enhance characteristics such as brittleness, hydrophobicity, degradation rate, and biocompatibility, within the composite. This review delves into the diverse roles that PHB, its alloys, and its compounds play in the engineering of skin, nerve, cartilage, and bone tissues. We explain these roles by highlighting key methodologies for crafting 3D tissue scaffolds with widely adopted techniques, including polymeric sponges, electrospinning, and salt leaching.

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Section: Studiesmentioning

confidence: 99%

The role of poly(3‐hydroxybutyrate)‐based derivatives in skin, nerve, cartilage, and bone tissue engineering applications—An updated review

Reyna‐Urrutia,

Leyva‐Gómez,

Ríos

et al. 2024

Polymers for Advanced Techs

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“…Polymers and inorganic substances that are used to manufacture PHB composites impact the osteogenic properties of scaffolds made of them. For example, polydopamine coating of P4HB copolymer scaffolds manufactured by rapid 3D prototyping allowed to functionalize scaffolds with growth factors such as BMP-2 [ 79 ]. Addition of nanosized hydroxyapatite to scaffolds made of a PHB–keratin composite results in an improvement in the attachment of osteoblast-like cells of the MG-63 line and an increase in the activity of alkaline phosphatase therein [ 76 ].…”

Section: Scaffolds From Poly(3-hydroxybutyrate) and Its Copolymers Fo...mentioning

confidence: 99%

“…Addition of nanosized hydroxyapatite to scaffolds made of a PHB–keratin composite results in an improvement in the attachment of osteoblast-like cells of the MG-63 line and an increase in the activity of alkaline phosphatase therein [ 76 ]. The coating of 3D-printed P4HB scaffolds with polydopamine developed their ability to enhance osteogenic differentiation of MSCs in an osteogenic environment, which was demonstrated by an increase in alkaline phosphatase activity, an increase in calcium salt deposition, and an increase in osteocalcin expression in cells [ 79 ].…”

Section: Scaffolds From Poly(3-hydroxybutyrate) and Its Copolymers Fo...mentioning

confidence: 99%

“…A study on a single critical bone defect of the rat parietal bone with a diameter of 5 mm [ 79 ] demonstrated that 3D-printed scaffolds made of P4HB and coated with polydopamine with the specifically adhered human recombinant growth factor BMP-2 had an express ability to accelerate bone tissue regeneration; this was less true for scaffolds made of P4HB and uncoated or coated with polydopamine, and true to a greater extent for BMP-2 scaffolds. In their study, Higuchi et al [ 88 ] made a similar defect in the lower jaw of a rat, and then introduced a scaffold of a PLGA composite and collagen loaded with BMP-2.…”

Section: Scaffolds Based On Poly(3-hydroxybutyrate) and Its Copolymer...mentioning

confidence: 99%

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Scaffolds Based on Poly(3-Hydroxybutyrate) and Its Copolymers for Bone Tissue Engineering (Review)

Bonartsev¹,

Voinova²,

Volkov³

et al. 2022

Sovrem Tehnol Med

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Biodegradable and biocompatible polymers are actively used in tissue engineering to manufacture scaffolds. Biomedical properties of polymer scaffolds depend on the physical and chemical characteristics and biodegradation kinetics of the polymer material, 3D microstructure and topography of the scaffold surface, as well as availability of minerals, medicinal agents, and growth factors loaded into the scaffold. However, in addition to the above, the intrinsic biological activity of the polymer and its biodegradation products can also become evident. This review provides studies demonstrating that scaffolds made of poly(3-hydroxybutyrate) (PHB) and its copolymers have their own biological activity, and namely, osteoinductive properties. PHB can induce differentiation of mesenchymal stem cells in the osteogenic direction in vitro and stimulates bone tissue regeneration during the simulation of critical and non-critical bone defects in vivo.

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“…Pure P3HB is considered too brittle for many applications, but its copolymers with monomers such as 4HB, 3HV, and 3HHx have superior mechanical properties compared to pure P3HB homopolymers. These copolymers are also being actively studied in the field of tissue reconstruction [ 52 , 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Ryltseva

Dudaev

Menzyanova

et al. 2023

JFB

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The needs of modern regenerative medicine for biodegradable polymers are wide and varied. Restoration of the viability of the vascular tree is one of the most important components of the preservation of the usefulness of organs and tissues. The creation of vascular implants compatible with blood is an important task of vascular bioengineering. The function of the endothelial layer of the vessel, being largely responsible for the development of thrombotic complications, is of great importance for hemocompatibility. The development of surfaces with specific characteristics of biomaterials that are used in vascular technologies is one of the solutions for their correct endothelialization. Linear polyhydroxyalkanoates (PHAs) are biodegradable structural polymeric materials suitable for obtaining various types of implants and tissue engineering, having a wide range of structural and physicomechanical properties. The use of PHA of various monomeric compositions in endothelial cultivation makes it possible to evaluate the influence of material properties, especially surface characteristics, on the functional state of cells. It has been established that PHA samples with the inclusion of 3-hydroxyhexanoate have optimal characteristics for the formation of a human umbilical vein endothelial cell, HUVEC, monolayer in terms of cell morphology as well as the levels of expression of vinculin and VE-cadherin. The obtained results provide a rationale for the use of PHA copolymers as materials for direct contact with the endothelium in vascular implants.

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Enhanced bone regeneration via PHA scaffolds coated with polydopamine-captured BMP2

Cited by 14 publications

References 66 publications

The role of poly(3‐hydroxybutyrate)‐based derivatives in skin, nerve, cartilage, and bone tissue engineering applications—An updated review

The role of poly(3‐hydroxybutyrate)‐based derivatives in skin, nerve, cartilage, and bone tissue engineering applications—An updated review

Scaffolds Based on Poly(3-Hydroxybutyrate) and Its Copolymers for Bone Tissue Engineering (Review)

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

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