Enhanced Bone Regeneration by Bone Morphogenetic Protein-2 after Pretreatment with Low-Intensity Pulsed Ultrasound in Distraction Osteogenesis

Han, Jeong Joon; Yang, Hoon-Joo; Hwang, Soon Jung

doi:10.1007/s13770-022-00457-1

Cited by 9 publications

(6 citation statements)

References 36 publications

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“…Lai et al ( 26 ) reported that no significant differences in expression of bone related genes among human MSCs treated with LIPUS or BMP2 alone and as co-treatments. Recently, a study by Han et al ( 27 ) repor-ted co-treatment of rat mesenchymal stem cells with BMP2 and LIPUS led to enhanced ALP activity compared to LIPUS alone or BMP2 alone. In vivo , it has also been reported that the co-stimulation with BMP2 and LIPUS enhanced bone formation ( 28 , 29 ).…”

Section: Discussionmentioning

confidence: 99%

Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

2023

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Dedifferentiated fat cells (DFATs) isolated from mature adipocytes have a multilineage differentiation capacity similar to mesenchymal stem cells and are considered as promising source of cells for tissue engineering. Bone morphogenetic protein 9 (BMP9) and low-intensity pulsed ultrasound (LIPUS) have been reported to stimulate bone formation both in vitro and in vivo . However, the combined effect of BMP9 and LIPUS on osteoblastic differentiation of DFATs has not been studied. After preparing DFATs from mature adipose tissue from rats, DFATs were treated with different doses of BMP9 and/or LIPUS. The effects on osteoblastic differentiation were assessed by changes in alkaline phosphatase (ALP) activity, mineralization/calcium deposition, and expression of bone related genes; Runx2, osterix, osteo-pontin. No significant differences for ALP activity, mineralization deposition, as well as expression for bone related genes were observed by LIPUS treatment alone while treatment with BMP9 induced osteoblastic differentiation of DFATs in a dose dependent manner. Further, co-treatment with BMP9 and LIPUS significantly increased osteoblastic differentiation of DFATs compared to those treated with BMP9 alone. In addition, upregulation for BMP9-receptor genes was observed by LIPUS treatment. Indomethacin, an inhibitor of prostaglandin synthesis, significantly inhibited the synergistic effect of BMP9 and LIPUS co-stimulation on osteoblastic differentiation of DFATs. LIPUS promotes BMP9 induced osteoblastic differentiation of DFATs in vitro and prostaglandins may be involved in this mechanism.

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Section: Discussionmentioning

confidence: 99%

Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

2023

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“…Han et al. ( 67 ) treated rat MSCs with LIPUS at a power of 60 mW/cm 2 and a frequency of 1.5 MHz in combination with BMP-2 and assessed relevant proliferation and osteogenic indicators. They found that the combination more strongly promoted cell proliferation, and they validated the effect of the combination treatment in a rabbit distraction osteogenesis model.…”

Section: Effects Of Lipus On the Biological Functions Of Stem/progeni...mentioning

confidence: 99%

Latest progress in low-intensity pulsed ultrasound for studying exosomes derived from stem/progenitor cells

He,

Wang,

Deng

et al. 2023

Front. Endocrinol.

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Stem cells have self-renewal, replication, and multidirectional differentiation potential, while progenitor cells are undifferentiated, pluripotent or specialized stem cells. Stem/progenitor cells secrete various factors, such as cytokines, exosomes, non-coding RNAs, and proteins, and have a wide range of applications in regenerative medicine. However, therapies based on stem cells and their secreted exosomes present limitations, such as insufficient source materials, mature differentiation, and low transplantation success rates, and methods addressing these problems are urgently required. Ultrasound is gaining increasing attention as an emerging technology. Low-intensity pulsed ultrasound (LIPUS) has mechanical, thermal, and cavitation effects and produces vibrational stimuli that can lead to a series of biochemical changes in organs, tissues, and cells, such as the release of extracellular bodies, cytokines, and other signals. These changes can alter the cellular microenvironment and affect biological behaviors, such as cell differentiation and proliferation. Here, we discuss the effects of LIPUS on the biological functions of stem/progenitor cells, exosomes, and non-coding RNAs, alterations involved in related pathways, various emerging applications, and future perspectives. We review the roles and mechanisms of LIPUS in stem/progenitor cells and exosomes with the aim of providing a deeper understanding of LIPUS and promoting research and development in this field.

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“…It is a photosensitive biohydrogel material that can form a 3D structure suitable for cell growth and differentiation with a certain strength via a curing reaction. 5 However, GelMA hydrogels lack osteoinductivity and often require the addition of relevant induction components such as hydroxyapatite, 6 bioceramics, 7 BMP, 8,9 and nanoclay. 10 Herford et al 11 suggested that the addition of rhBMP-2 may enhance healing at the osteotomy site and enhance results obtained by the sandwich osteotomy.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional bioprinted GelMA/GO composite hydrogel for stem cell osteogenic differentiation both in vitro and in vivo

Jiang,

Zhou,

Jiang

2024

J Biomater Appl

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In this study, we evaluated the use of graphene oxide (GO) mixed with methyl methacrylate gelatin (GelMA) for the construction of a microenvironmental implant to repair bone defects in orthopedic surgery. A scaffold containing a GelMA/GO composite with mesenchymal stem cells (MSCs) was constructed using three-dimensional bioprinting. The survival and osteogenic capacity of MSCs in the composite bioink were evaluated using cell viability and proliferation assays, osteogenesis-related gene expression analysis, and implantation under the skin of nude mice. The printing process had little effect on cell viability. We found that GO enhanced cell proliferation but had no significant effect on cell viability. In vitro experiments suggested that GO promoted material–cell interactions and the expression of osteogenesis-related genes. In vivo experiments showed that GO decreased the degradation time of the material and increased calcium nodule deposition. In contrast to pure GelMA, the addition of GO created a suitable microenvironment to promote the differentiation of loaded exogenous MSCs in vitro and in vivo, providing a basis for the repair of bone defects.

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Enhanced Bone Regeneration by Bone Morphogenetic Protein-2 after Pretreatment with Low-Intensity Pulsed Ultrasound in Distraction Osteogenesis

Cited by 9 publications

References 36 publications

Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

Latest progress in low-intensity pulsed ultrasound for studying exosomes derived from stem/progenitor cells

Three-dimensional bioprinted GelMA/GO composite hydrogel for stem cell osteogenic differentiation both in vitro and in vivo

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