Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials

Lee, Jue Yeon; Nam, So Hee; Im, Su Yeon; Park, Yoon Jeong; Lee, Yong-Moo; Seol, Yang‐Jo; Chung, Chong Pyoung; Lee, Seung Jin

doi:10.1016/s0168-3659(01)00498-9

Cited by 278 publications

(149 citation statements)

References 42 publications

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“…Owing to the rapid advances in recombinant technology and the availability of large scale manufacturing of cytokines and growth factors, many recent tissue engineering strategies have turned to the use of specific growth factors to stimulate cellular activity in vitro and for improved functional neotissue formation in vivo [2][3][4][5]. Importantly, the delivery mode appears to be critical for the application of these factors in tissue engineering [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Nano-fibrous scaffold for controlled delivery of recombinant human PDGF-BB

Wei

Jin

Giannobile

et al. 2006

Journal of Controlled Release

202

157

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The localized and temporally controlled delivery of growth factors is key to achieving optimal clinical efficacy. In sophisticated tissue engineering strategies, the biodegradable scaffold is preferred to serve as both a three-dimensional (3-D) substrate and a growth factor delivery vehicle to promote cellular activity and enhance tissue neogenesis. This study presents a novel approach to fabricate tissue engineering scaffolds capable of controlled growth factor delivery whereby growth factor containing microspheres were incorporated into 3-D scaffolds with good mechanical properties, wellinterconnected macroporous and nano-fibrous structures. The microspheres were uniformly distributed throughout the nano-fibrous scaffold and their incorporation did not interfere the macro-, micro-, and nanostructures of the scaffold. The release kinetics of platelet-derived growth factor-BB (PDGF-BB) from microspheres and scaffolds was investigated using poly(lactic-co-glycolic acid) (PLGA50) microspheres with different molecular weights (6.5 and 64kDa, respectively) and microsphere-incorporated poly(L-lactic acid) (PLLA) nano-fibrous scaffolds. Incorporation of microspheres into scaffolds significantly reduced the initial burst release. Sustained release from several days to months was achieved through different microspheres in scaffolds. Released PDGF-BB was demonstrated to possess biological activity as evidenced by stimulation of human gingival fibroblast DNA synthesis in vitro. The successful generation of 3-D nano-fibrous scaffold incorporating controlled-release factors indicates significant potential for more complex tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Nano-fibrous scaffold for controlled delivery of recombinant human PDGF-BB

Wei

Jin

Giannobile

et al. 2006

Journal of Controlled Release

202

157

View full text Add to dashboard Cite

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“…Modification of poly-(lactic acid) devices that release bone morpho genetic protein 2 (BMP2) 83 and chitosan enhanced bone regeneration due to an increase in cell adhesion to the polymer matrix. Furthermore, coupling oligopeptides that contain the RGD sequence to protein delivery vehicles may also improve the cellular response to growth factors by facilitating cell migration into the material, activating the cells to the desired responsive state and subsequently exposing the cells to the drug in a controlled manner 84 .…”

Section: Antisense Therapymentioning

confidence: 99%

Microenvironmental regulation of biomacromolecular therapies

Kong

Mooney

2007

Nat Rev Drug Discov

137

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“…The physical properties of a polymer can be altered by introducing a second polymer that improves the properties of the original polymer in certain aspects, such as hydrophobility, cell compatibility. CS and some of its complexes have been studied for a number of biomedical applications, including wound dressings, drug delivery systems and space-filling implants [12][13][14][15][16][17][18]. In a study comparing purified collagen, naturally occurring extracellular matrix (ECM) scaffolds, and synthetic scaffold materials for in vitro endothelial cell attachment [19], it was found that ECM possessed the ability to recruit circulating marrow-derived progenitor cells and attract mature endothelial cells from selected organs such as the heart and liver to promote successful vascularization of engineered tissue structures.…”

Section: Introductionmentioning

confidence: 99%

Preparation, properties, and cell attachment/growth behavior of chitosan/acellular derm matrix composite materials

Lü¹,

Li²,

Zhang³

et al. 2011

JBNB

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Composite membranes and sponge scaffolds consisting chitosan (CS) and acellular derm matrix (ADM) in six ratios were prepared by solvent evaporation technique and freeze-drying method, respectively. The composite materials were characterized by water contact angle measurement, scanning electron microscopy (SEM), water absorption and HaCat cells compatibility. The SEM result showed that CS/ADM three-dimensional (3D) micro-porous structures were successfully produced. The water absorption value of all scaffolds was over 18 times of its initial weight, which is high enough for skin regeneration scaffold, but there were no significant differences of water absorption ratio between deionized water and PBS solution for same scaffold (P > 0.05). HaCat cells were distributed uniformly on the surfaces of membrane 4 -6, and an almost confluent monolayer was formed on membrane 6 on the fifth day, whereas cells maintained round and spherical in shape on the surface of membrane 1. The results showed that the cell compatibility of pure CS membrane needed to be improved, and addition of ADM realized this purpose. The results of compatibility of HaCat cells on scaffolds showed that the cell proliferated well on the scaffolds 3 and 4. In our study, the cell's attachment and growth on the composite membranes was mainly determined by the content of the membrane, whereas the cell's attachment and growth in the scaffolds was determined by both the content and structure of the scaffolds.

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Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials

Cited by 278 publications

References 42 publications

Nano-fibrous scaffold for controlled delivery of recombinant human PDGF-BB

Nano-fibrous scaffold for controlled delivery of recombinant human PDGF-BB

Microenvironmental regulation of biomacromolecular therapies

Preparation, properties, and cell attachment/growth behavior of chitosan/acellular derm matrix composite materials

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